RID=1168926706-11248-20906931483.BLASTQ1, Contig21

BLASTX 2.2.15 [Oct-15-2006]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. RID: 1168926706-11248-20906931483.BLASTQ1 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples 4,458,890 sequences; 1,532,996,420 total letters

If you have any problems or questions with the results of this search
please refer to the BLAST FAQs Taxonomy reports

Query=  Contig21
Length=583

Distribution of 14 Blast Hits on the Query Sequence

                                                                   Score     E
Sequences producing significant alignments:                       (Bits)  Value

gi|108709561|gb|ABF97356.1|  Lysyl-tRNA synthetase, putative, ...   139    7e-33
gi|115453943|ref|NP_001050572.1|  Os03g0586800 [Oryza sativa (...   139    7e-33 
gi|15229833|ref|NP_187777.1|  ATP binding / aspartate-tRNA lig...   134    2e-31 
gi|92882121|gb|ABE86616.1|  Lysyl-tRNA synthetase, class-2; Am...   130    4e-30
gi|92876634|gb|ABE84004.1|  Lysyl-tRNA synthetase, class-2; Am...   130    4e-30
gi|2501025|sp|Q43776|SYK_LYCES  Lysyl-tRNA synthetase (Lysine-...   118    1e-26 
gi|108709562|gb|ABF97357.1|  Lysyl-tRNA synthetase, putative, ...   115    1e-25
gi|116054887|emb|CAL56964.1|  Aspartyl-tRNA synthetase (ISS) [Ost  85.1    2e-16
gi|115447385|ref|NP_001047472.1|  Os02g0623500 [Oryza sativa (...  39.3    0.010 
gi|15231261|ref|NP_187958.1|  ATP binding / aspartate-tRNA lig...  37.7    0.030 
gi|92882120|gb|ABE86615.1|  hypothetical protein MtrDRAFT_AC15...  37.4    0.039
gi|92876635|gb|ABE84005.1|  hypothetical protein MtrDRAFT_AC12...  37.4    0.039

Alignments

 

>gi|108709561|gb|ABF97356.1|  Lysyl-tRNA synthetase, putative, expressed [Oryza sativa (japonica 
cultivar-group)]
Length=595

 Score =  139 bits (350),  Expect = 7e-33
 Identities = 70/110 (63%), Positives = 81/110 (73%), Gaps = 0/110 (0%)
 Frame = +1

Query  199  AAAMTSSQSQKPSSAEDEDMDPTQYYESRLKALASLKAVGVNPYPHKFLVSMSVVDYVEK  378
            A A  S +  K S+A+DE+MDPTQYYE+RLKAL SLKA GVNPYPHKFL +++V DY+EK
Sbjct  58   ATAAASGEPPKESAADDEEMDPTQYYENRLKALDSLKATGVNPYPHKFLANITVADYIEK  117

Query  379  YGRLRDGEHLKDVEVSLAXXIMNKRASSSKLYFYDLYGDGVKVXVMADAR  528
            Y  +  G+ L DV   LA  IM KRA SSKL FYDLYG G KV V ADAR
Sbjct  118  YKSMNVGDKLVDVTECLAGRIMTKRAQSSKLLFYDLYGGGEKVQVFADAR  167


>gi|115453943|ref|NP_001050572.1|  Os03g0586800 [Oryza sativa (japonica cultivar-group)]
 gi|55583785|sp|Q6F2U9|SYK_ORYSA  Lysyl-tRNA synthetase (Lysine--tRNA ligase) (LysRS)
 gi|50399950|gb|AAT76338.1|  putative Lysyl-tRNA synthetase [Oryza sativa (japonica cultivar-group)]
 gi|108709560|gb|ABF97355.1|  Lysyl-tRNA synthetase, putative, expressed [Oryza sativa (japonica 
cultivar-group)]
 gi|113549043|dbj|BAF12486.1|  Os03g0586800 [Oryza sativa (japonica cultivar-group)]
Length=602

 Score =  139 bits (350),  Expect = 7e-33
 Identities = 70/110 (63%), Positives = 81/110 (73%), Gaps = 0/110 (0%)
 Frame = +1

Query  199  AAAMTSSQSQKPSSAEDEDMDPTQYYESRLKALASLKAVGVNPYPHKFLVSMSVVDYVEK  378
            A A  S +  K S+A+DE+MDPTQYYE+RLKAL SLKA GVNPYPHKFL +++V DY+EK
Sbjct  58   ATAAASGEPPKESAADDEEMDPTQYYENRLKALDSLKATGVNPYPHKFLANITVADYIEK  117

Query  379  YGRLRDGEHLKDVEVSLAXXIMNKRASSSKLYFYDLYGDGVKVXVMADAR  528
            Y  +  G+ L DV   LA  IM KRA SSKL FYDLYG G KV V ADAR
Sbjct  118  YKSMNVGDKLVDVTECLAGRIMTKRAQSSKLLFYDLYGGGEKVQVFADAR  167


>gi|15229833|ref|NP_187777.1|  ATP binding / aspartate-tRNA ligase/ lysine-tRNA ligase/ nucleic 
acid binding / tRNA ligase [Arabidopsis thaliana]
 gi|55583786|sp|Q9ZPI1|SYK_ARATH  Lysyl-tRNA synthetase (Lysine--tRNA ligase) (LysRS)
 gi|6041823|gb|AAF02138.1|AC009918_10  lysyl-tRNA synthetase [Arabidopsis thaliana]
 gi|4325324|gb|AAD17333.1|  lysyl-tRNA synthetase; LysRS [Arabidopsis thaliana]
 gi|20466171|gb|AAM20403.1|  lysyl-tRNA synthetase [Arabidopsis thaliana]
 gi|25083918|gb|AAN72134.1|  lysyl-tRNA synthetase [Arabidopsis thaliana]
Length=626

 Score =  134 bits (337),  Expect = 2e-31
 Identities = 65/103 (63%), Positives = 82/103 (79%), Gaps = 0/103 (0%)
 Frame = +1

Query  217  SQSQKPSSAEDEDMDPTQYYESRLKALASLKAVGVNPYPHKFLVSMSVVDYVEKYGRLRD  396
            + SQK  +A+DE+MD TQYYE+RLK LA+ KA G NPYPHKF VSMS+  Y+E YG L +
Sbjct  72   ASSQKAVAADDEEMDATQYYENRLKYLAAEKAKGENPYPHKFAVSMSIPKYIETYGSLNN  131

Query  397  GEHLKDVEVSLAXXIMNKRASSSKLYFYDLYGDGVKVXVMADA  525
            G+H+++ E SLA  IM+KR+SSSKL+FYDL+GD  KV VMADA
Sbjct  132  GDHVENAEESLAGRIMSKRSSSSKLFFYDLHGDDFKVQVMADA  174


>gi|92882121|gb|ABE86616.1|  Lysyl-tRNA synthetase, class-2; Aminoacyl-transfer RNA synthetase, 
class II [Medicago truncatula]
Length=1099

 Score =  130 bits (326),  Expect = 4e-30
 Identities = 62/110 (56%), Positives = 83/110 (75%), Gaps = 1/110 (0%)
 Frame = +1

Query  199  AAAMTSSQSQKPSSAEDEDMDPTQYYESRLKALASLKAVGVNPYPHKFLVSMSVVDYVEK  378
            AA +  ++  +P++A DEDMDP +Y E+RLK LA+LK  G+NPYPHKF VSM +V Y+++
Sbjct  522  AAEIQPARPNRPAAA-DEDMDPKEYRENRLKYLAALKTEGINPYPHKFSVSMPIVQYIDR  580

Query  379  YGRLRDGEHLKDVEVSLAXXIMNKRASSSKLYFYDLYGDGVKVXVMADAR  528
            YG L +GEHL+D+ +SLA  IM+KR S  KL+FYDL+G   KV VMAD R
Sbjct  581  YGGLSNGEHLEDMSISLAGRIMHKRKSGKKLFFYDLHGGAFKVQVMADVR  630


 Score = 52.0 bits (123),  Expect = 2e-06
 Identities = 31/56 (55%), Positives = 39/56 (69%), Gaps = 3/56 (5%)
 Frame = +1

Query  358  VVDYVEKYGRLRDGEHLKDVEVSLAXXIMNKRASSSKLYFYDLYGDGVKVXVMADA  525
            ++ Y + Y  L +GEHL+ V VSLA  IMNK +S +KL FYDL G GVK  V+ADA
Sbjct  89   ILQYNDIYQGLSNGEHLEGVSVSLAGQIMNKDSSDAKLVFYDLKG-GVK--VIADA  141


>gi|92876634|gb|ABE84004.1|  Lysyl-tRNA synthetase, class-2; Aminoacyl-transfer RNA synthetase, 
class II [Medicago truncatula]
Length=1093

 Score =  130 bits (326),  Expect = 4e-30
 Identities = 62/110 (56%), Positives = 83/110 (75%), Gaps = 1/110 (0%)
 Frame = +1

Query  199  AAAMTSSQSQKPSSAEDEDMDPTQYYESRLKALASLKAVGVNPYPHKFLVSMSVVDYVEK  378
            AA +  ++  +P++A DEDMDP +Y E+RLK LA+LK  G+NPYPHKF VSM +V Y+++
Sbjct  516  AAEIQPARPNRPAAA-DEDMDPKEYRENRLKYLAALKTEGINPYPHKFSVSMPIVQYIDR  574

Query  379  YGRLRDGEHLKDVEVSLAXXIMNKRASSSKLYFYDLYGDGVKVXVMADAR  528
            YG L +GEHL+D+ +SLA  IM+KR S  KL+FYDL+G   KV VMAD R
Sbjct  575  YGGLSNGEHLEDMSISLAGRIMHKRKSGKKLFFYDLHGGAFKVQVMADVR  624


 Score = 52.0 bits (123),  Expect = 2e-06
 Identities = 31/56 (55%), Positives = 39/56 (69%), Gaps = 3/56 (5%)
 Frame = +1

Query  358  VVDYVEKYGRLRDGEHLKDVEVSLAXXIMNKRASSSKLYFYDLYGDGVKVXVMADA  525
            ++ Y + Y  L +GEHL+ V VSLA  IMNK +S +KL FYDL G GVK  V+ADA
Sbjct  76   ILQYNDIYQGLSNGEHLEGVSVSLAGQIMNKDSSDAKLVFYDLKG-GVK--VIADA  128


>gi|2501025|sp|Q43776|SYK_LYCES  Lysyl-tRNA synthetase (Lysine--tRNA ligase) (LysRS)
 gi|1147585|emb|CAA64223.1|  Lysyl-tRNA synthetase [Solanum lycopersicum]
Length=588

 Score =  118 bits (296),  Expect = 1e-26
 Identities = 52/110 (47%), Positives = 81/110 (73%), Gaps = 0/110 (0%)
 Frame = +1

Query  199  AAAMTSSQSQKPSSAEDEDMDPTQYYESRLKALASLKAVGVNPYPHKFLVSMSVVDYVEK  378
            AAA+   Q ++ +  E++D+DPTQY  +RL+ + SL+  G+NPYPHKF ++MS+ +++ +
Sbjct  33   AAAVAKRQMEQHNLPENDDLDPTQYLANRLRNIESLRESGINPYPHKFFITMSIPEFISR  92

Query  379  YGRLRDGEHLKDVEVSLAXXIMNKRASSSKLYFYDLYGDGVKVXVMADAR  528
            Y  L  GE  +D+++SLA  +++KRASSSKLYFY+L G G +V V+A AR
Sbjct  93   YAHLNTGEFPEDIDMSLAGRVISKRASSSKLYFYELLGGGARVQVLASAR  142


>gi|108709562|gb|ABF97357.1|  Lysyl-tRNA synthetase, putative, expressed [Oryza sativa (japonica 
cultivar-group)]
Length=528

 Score =  115 bits (288),  Expect = 1e-25
 Identities = 58/87 (66%), Positives = 65/87 (74%), Gaps = 0/87 (0%)
 Frame = +1

Query  268  QYYESRLKALASLKAVGVNPYPHKFLVSMSVVDYVEKYGRLRDGEHLKDVEVSLAXXIMN  447
            QYYE+RLKAL SLKA GVNPYPHKFL +++V DY+EKY  +  G+ L DV   LA  IM 
Sbjct  7    QYYENRLKALDSLKATGVNPYPHKFLANITVADYIEKYKSMNVGDKLVDVTECLAGRIMT  66

Query  448  KRASSSKLYFYDLYGDGVKVXVMADAR  528
            KRA SSKL FYDLYG G KV V ADAR
Sbjct  67   KRAQSSKLLFYDLYGGGEKVQVFADAR  93


>gi|116054887|emb|CAL56964.1|  Aspartyl-tRNA synthetase (ISS) [Ostreococcus tauri]
Length=593

 Score = 85.1 bits (209),  Expect = 2e-16
 Identities = 47/109 (43%), Positives = 61/109 (55%), Gaps = 3/109 (2%)
 Frame = +1

Query  202  AAMTSSQSQKPSSAED--EDMDPTQYYESRLKALASLKAVGVNPYPHKFLVSMSVVDYVE  375
            A    +Q  K  S ED   D+DP QY   R+K + + K+ G  PYPHKF V     +++ 
Sbjct  59   AEAAKAQPAKEKSEEDLEVDLDPAQYTAGRIKKVVAAKSRGEAPYPHKFHVDTRFEEFIA  118

Query  376  KYGRLRDGEHLKDVEVSLAXXIMNKRASSSKLYFYDLYGDGVKVXVMAD  522
            KY  L +G    D   S+A  IM+KRAS  KLYFYDL  DG K+ + AD
Sbjct  119  KYSSLPEG-GAADETCSIAGRIMSKRASGKKLYFYDLVADGKKIQITAD  166


>gi|115447385|ref|NP_001047472.1|  Os02g0623500 [Oryza sativa (japonica cultivar-group)]
 gi|48716234|dbj|BAD23770.1|  putative lysyl-tRNA synthetase [Oryza sativa (japonica cultivar-group)]
 gi|113537003|dbj|BAF09386.1|  Os02g0623500 [Oryza sativa (japonica cultivar-group)]
Length=586

 Score = 39.3 bits (90),  Expect = 0.010
 Identities = 27/87 (31%), Positives = 41/87 (47%), Gaps = 1/87 (1%)
 Frame = +1

Query  235  SSAEDEDMDPTQYYESRLKALASLKAVGVNPYPHKFLVSMSVVDYVEKYGRLRDGEHLKD  414
            SS+     D       RLK +  L+  G  PY +K+  + +  +  + Y  L +GE   D
Sbjct  62   SSSSSSTSDRESIRAIRLKKVEELRGKGYEPYAYKWDRTHTTKELQDGYTHLENGEVCAD  121

Query  415  VEVSLAXXIMNKRASSSKLYFYDLYGD  495
            V VS+A  I+ +RA   KL F  +  D
Sbjct  122  VAVSIAGRIVARRA-FGKLVFMTVRDD  147


>gi|15231261|ref|NP_187958.1|  ATP binding / aspartate-tRNA ligase/ lysine-tRNA ligase/ nucleic 
acid binding / tRNA ligase [Arabidopsis thaliana]
 gi|9280301|dbj|BAB01756.1|  lysyl-tRNA synthetase [Arabidopsis thaliana]
 gi|26983796|gb|AAN86150.1|  putative lysyl-tRNA synthetase [Arabidopsis thaliana]
Length=602

 Score = 37.7 bits (86),  Expect = 0.030
 Identities = 34/122 (27%), Positives = 56/122 (45%), Gaps = 12/122 (9%)
 Frame = +1

Query  199  AAAMTSSQSQKPS-------SAEDEDMDPTQYYESRLKALASLKAVGVNPYPHKFLVSMS  357
            +++ T++++ KPS       S+ +   D       RLK +  L+  G+ PY +K+  S S
Sbjct  53   SSSATTAETSKPSGRNRRSASSSNSTSDREAIRSIRLKKVEELRGQGLEPYAYKWEKSHS  112

Query  358  VVDYVEKYGRLRDGEHLKDVE---VSLAXXIMNKRASSSKLYFYDLYGDGVKVXVMADAR  528
                 E Y  L +GE   D E   VS+A  ++ +RA   KL F  L  D   + +  +  
Sbjct  113  ANQLQEIYKHLANGEE-SDNEIDCVSIAGRVVARRA-FGKLAFLTLRDDSGTIQLYCEKE  170

Query  529  XL  534
             L
Sbjct  171  RL  172


>gi|92882120|gb|ABE86615.1|  hypothetical protein MtrDRAFT_AC159873g11v1 [Medicago truncatula]
Length=132

 Score = 37.4 bits (85),  Expect = 0.039
 Identities = 17/33 (51%), Positives = 23/33 (69%), Gaps = 0/33 (0%)
 Frame = +1

Query  265  TQYYESRLKALASLKAVGVNPYPHKFLVSMSVV  363
            T Y E+RLK LAS K  G +PYPHK  V ++++
Sbjct  99   TPYTENRLKYLASQKTEGNDPYPHKLSVPITIL  131


>gi|92876635|gb|ABE84005.1|  hypothetical protein MtrDRAFT_AC125475g14v1 [Medicago truncatula]
Length=49

 Score = 37.4 bits (85),  Expect = 0.039
 Identities = 17/33 (51%), Positives = 23/33 (69%), Gaps = 0/33 (0%)
 Frame = +1

Query  265  TQYYESRLKALASLKAVGVNPYPHKFLVSMSVV  363
            T Y E+RLK LAS K  G +PYPHK  V ++++
Sbjct  16   TPYTENRLKYLASQKTEGNDPYPHKLSVPITIL  48

  Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding 
environmental samples
    Posted date:  Jan 15, 2007  4:53 AM
  Number of letters in database: 101,054,579
  Number of sequences in database:  283,349
Lambda     K      H
   0.317    0.130    0.359 
Gapped
Lambda     K      H
   0.267   0.0410    0.140 
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 283349
Number of Hits to DB: 240
Number of extensions: 2
Number of successful extensions: 0
Number of sequences better than 10: 0
Number of HSP's better than 10 without gapping: 0
Number of HSP's gapped: 0
Number of HSP's successfully gapped: 0
Length of query: 583
Length of database: 101054579
Length adjustment: 107
Effective length of query: 476
Effective length of database: 70736236
Effective search space: 6154052532
Effective search space used: 6154052532
T: 12
A: 40
X1: 16 (7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (20.4 bits)
S2: 64 (29.3 bits)