RID=1168944812-1428-205450100627.BLASTQ1, Contig309

BLASTX 2.2.15 [Oct-15-2006]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. RID: 1168944812-1428-205450100627.BLASTQ1 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples 4,458,890 sequences; 1,532,996,420 total letters

If you have any problems or questions with the results of this search
please refer to the BLAST FAQs Taxonomy reports

Query=  Contig309
Length=542

Distribution of 10 Blast Hits on the Query Sequence

Related Structures

                                                                   Score     E
Sequences producing significant alignments:                       (Bits)  Value

gi|15234000|ref|NP_195022.1|  unknown protein [Arabidopsis tha...   210    2e-54 
gi|38564256|gb|AAR23707.1|  At4g32960 [Arabidopsis thaliana]        206    5e-53 
gi|3688178|emb|CAA21206.1|  putative protein [Arabidopsis thal...   186    4e-47
gi|30689557|ref|NP_195023.2|  unknown protein [Arabidopsis thalia   186    4e-47 
gi|115466444|ref|NP_001056821.1|  Os06g0150100 [Oryza sativa (...   158    1e-38 
gi|115481100|ref|NP_001064143.1|  Os10g0141400 [Oryza sativa (...  34.7    0.22  
gi|2981956|pdb|1BRY|Y  Chain Y, Bryodin Type I Rip >gi|2981957...  31.6    1.8   
gi|12644214|sp|P33185|RIP1_BRYDI  Ribosome-inactivating protei...  31.6    1.8  
gi|15236271|ref|NP_192234.1|  DNA binding / transcription fact...  30.4    4.1   
gi|15218851|ref|NP_171853.1|  unknown protein [Arabidopsis tha...  29.3    9.1

Alignments

 

>gi|15234000|ref|NP_195022.1|  unknown protein [Arabidopsis thaliana]
 gi|3688177|emb|CAA21205.1|  putative protein [Arabidopsis thaliana]
 gi|7270243|emb|CAB80013.1|  putative protein [Arabidopsis thaliana]
 gi|38603880|gb|AAR24685.1|  At4g32960 [Arabidopsis thaliana]
Length=264

 Score =  210 bits (535),  Expect = 2e-54
 Identities = 105/157 (66%), Positives = 129/157 (82%), Gaps = 6/157 (3%)
 Frame = -3

Query  456  GTGRYTLKPTRKSNEDIIFCVDVDAESLVEMKTAG-----ITRLDSIRQAILLFVNSKLS  292
            GT RY LKP R  +EDI+ C+DVDAES+VEMKT G     + R++ ++QAI+LF+++KLS
Sbjct  12   GTTRYALKPGRIKSEDILICIDVDAESMVEMKTTGTNGRPLIRMECVKQAIILFIHNKLS  71

Query  291  INPDHRFAFATLSKSASWLRKEFSSDVESTIAAVRALSATTVSSGQADLTNLFRLAAHEA  112
            INPDHRFAFATL+KSA+WL+KEF+SD ES +A++R LS    SS +ADLT LFR AA EA
Sbjct  72   INPDHRFAFATLAKSAAWLKKEFTSDAESAVASLRGLSGNK-SSSRADLTLLFRAAAQEA  130

Query  111  KKSRAQNRILRVILIYCRSSIRPQHQWPVNQKLFTLD  1
            K SRAQNRI RVILIYCRSS+RP H+WP+NQKLFTLD
Sbjct  131  KVSRAQNRIFRVILIYCRSSMRPTHEWPLNQKLFTLD  167


>gi|38564256|gb|AAR23707.1|  At4g32960 [Arabidopsis thaliana]
Length=264

 Score =  206 bits (523),  Expect = 5e-53
 Identities = 103/157 (65%), Positives = 128/157 (81%), Gaps = 6/157 (3%)
 Frame = -3

Query  456  GTGRYTLKPTRKSNEDIIFCVDVDAESLVEMKTAG-----ITRLDSIRQAILLFVNSKLS  292
            GT RY LKP R  +EDI+ C+DVDAES+VEMKT G     + R++ ++QAI+LF+++KLS
Sbjct  12   GTTRYALKPGRIKSEDILICIDVDAESMVEMKTTGTNGRPLIRMECVKQAIILFIHNKLS  71

Query  291  INPDHRFAFATLSKSASWLRKEFSSDVESTIAAVRALSATTVSSGQADLTNLFRLAAHEA  112
            INPDHRFAFATL+KSA+WL+KEF+S+ ES +A++R LS    SS +ADLT LFR AA  A
Sbjct  72   INPDHRFAFATLAKSAAWLKKEFTSNAESAVASLRGLSGNK-SSSRADLTLLFRAAAQGA  130

Query  111  KKSRAQNRILRVILIYCRSSIRPQHQWPVNQKLFTLD  1
            K SRAQNRI RVILIYCRSS+RP H+WP+NQKLFTLD
Sbjct  131  KVSRAQNRIFRVILIYCRSSMRPTHEWPLNQKLFTLD  167


>gi|3688178|emb|CAA21206.1|  putative protein [Arabidopsis thaliana]
 gi|7270244|emb|CAB80014.1|  putative protein [Arabidopsis thaliana]
Length=743

 Score =  186 bits (472),  Expect = 4e-47
 Identities = 97/156 (62%), Positives = 120/156 (76%), Gaps = 6/156 (3%)
 Frame = -3

Query  453  TGRYTLKPTRKSNEDIIFCVDVDAESLVEMKTAG-----ITRLDSIRQAILLFVNSKLSI  289
            TG    + + + +EDI+ CVDVDAES VEMKT G     + RL+ ++ AI  F++ KLS 
Sbjct  2    TGAVATRDSVEPSEDILICVDVDAESTVEMKTTGTNGKPLNRLECLKLAITRFIHDKLSR  61

Query  288  NPDHRFAFATLSKSASWLRKEFSSDVESTIAAVRALSATTVSSGQADLTNLFRLAAHEAK  109
            N DHRFAFATLS+SA+WL+KEFS+D +S  A++R +SAT  SSG ADLT LF+ AA EAK
Sbjct  62   NSDHRFAFATLSQSAAWLKKEFSNDAKSAAASLREMSATR-SSGPADLTFLFQEAAQEAK  120

Query  108  KSRAQNRILRVILIYCRSSIRPQHQWPVNQKLFTLD  1
             SRAQNRILRVIL+YCRSS+RP H WP+NQKLFTLD
Sbjct  121  TSRAQNRILRVILMYCRSSVRPTHDWPINQKLFTLD  156


>gi|30689557|ref|NP_195023.2|  unknown protein [Arabidopsis thaliana]
Length=750

 Score =  186 bits (472),  Expect = 4e-47
 Identities = 97/156 (62%), Positives = 120/156 (76%), Gaps = 6/156 (3%)
 Frame = -3

Query  453  TGRYTLKPTRKSNEDIIFCVDVDAESLVEMKTAG-----ITRLDSIRQAILLFVNSKLSI  289
            TG    + + + +EDI+ CVDVDAES VEMKT G     + RL+ ++ AI  F++ KLS 
Sbjct  9    TGAVATRDSVEPSEDILICVDVDAESTVEMKTTGTNGKPLNRLECLKLAITRFIHDKLSR  68

Query  288  NPDHRFAFATLSKSASWLRKEFSSDVESTIAAVRALSATTVSSGQADLTNLFRLAAHEAK  109
            N DHRFAFATLS+SA+WL+KEFS+D +S  A++R +SAT  SSG ADLT LF+ AA EAK
Sbjct  69   NSDHRFAFATLSQSAAWLKKEFSNDAKSAAASLREMSATR-SSGPADLTFLFQEAAQEAK  127

Query  108  KSRAQNRILRVILIYCRSSIRPQHQWPVNQKLFTLD  1
             SRAQNRILRVIL+YCRSS+RP H WP+NQKLFTLD
Sbjct  128  TSRAQNRILRVILMYCRSSVRPTHDWPINQKLFTLD  163


>gi|115466444|ref|NP_001056821.1|  Os06g0150100 [Oryza sativa (japonica cultivar-group)]
 gi|55297260|dbj|BAD69045.1|  unknown protein [Oryza sativa (japonica cultivar-group)]
 gi|113594861|dbj|BAF18735.1|  Os06g0150100 [Oryza sativa (japonica cultivar-group)]
Length=285

 Score =  158 bits (399),  Expect = 1e-38
 Identities = 80/179 (44%), Positives = 114/179 (63%), Gaps = 30/179 (16%)
 Frame = -3

Query  447  RYTLKPTRKSNEDIIFCVDVDAESLVEMKTAG----------------------------  352
            R+TL+P+R   ED++FCVDVD E+  EM+ A                             
Sbjct  14   RFTLQPSRLPPEDMLFCVDVDLETRSEMRIAPGPAAAAAASPGAAGASSGAAAASRQAAR  73

Query  351  --ITRLDSIRQAILLFVNSKLSINPDHRFAFATLSKSASWLRKEFSSDVESTIAAVRALS  178
              + R+D+++QA+LLFV+SKL++ PDHRFAFA+L  + S ++K+FSSD  S + A+++L 
Sbjct  74   PPVKRMDAVKQALLLFVHSKLTMCPDHRFAFASLGDTVSLVKKDFSSDAGSAVEAIQSLD  133

Query  177  ATTVSSGQADLTNLFRLAAHEAKKSRAQNRILRVILIYCRSSIRPQHQWPVNQKLFTLD  1
            A+      ADLT LF++A  E K++  Q R+LRV+LIYCRSS +PQHQWP+ QK FTLD
Sbjct  134  ASETRYAMADLTQLFKIAYQEGKRAELQGRLLRVVLIYCRSSTKPQHQWPIKQKNFTLD  192


>gi|115481100|ref|NP_001064143.1|  Os10g0141400 [Oryza sativa (japonica cultivar-group)]
 gi|16519461|gb|AAL25170.1|AC079852_3  Putative 26S proteasome regulatory subunit S5A [Oryza sativa]
 gi|19919978|gb|AAM08426.1|AC112513_12  Putative 26S proteasome regulatory subunit S5A [Oryza sativa]
 gi|31430116|gb|AAP52074.1|  26S proteasome regulatory subunit S5A, putative [Oryza sativa 
(japonica cultivar-group)]
 gi|113638752|dbj|BAF26057.1|  Os10g0141400 [Oryza sativa (japonica cultivar-group)]
Length=550

 Score = 34.7 bits (78),  Expect = 0.22
 Identities = 27/112 (24%), Positives = 53/112 (47%), Gaps = 5/112 (4%)
 Frame = -3

Query  348  TRLDSIRQAILLFVNSKLSINPDHRFAFATLSKSASWLRKEFSSDVESTIAAVRALSATT  169
            TRL +   A  L V +K++ NP++      ++     +    +SD    +A +  L A  
Sbjct  26   TRLQAQEDAANLVVGTKMTSNPENTVGVLAMAGDRVRVLLAPTSDPVKFLACMHGLEA--  83

Query  168  VSSGQADLTNLFRLAAHEAKKSRAQNRILRVILIYCRSSIRPQHQWPVNQKL  13
              SG+A+LT    + A    K+R   R+ + I+++  S ++ +    + +KL
Sbjct  84   --SGEANLTATLNI-AELVLKNRPDKRLSQRIVVFVGSPVKDEKLETIGKKL  132


>gi|2981956|pdb|1BRY|Y  Chain Y, Bryodin Type I Rip
 gi|2981957|pdb|1BRY|Z  Chain Z, Bryodin Type I Rip
Length=248

 Score = 31.6 bits (70),  Expect = 1.8
 Identities = 29/133 (21%), Positives = 57/133 (42%), Gaps = 5/133 (3%)
 Frame = -3

Query  468  ASVGGTGRYT-LKPTRKSNEDIIFCVDVDAESLVEMKTAGIT----RLDSIRQAILLFVN  304
            +S+ G+GRYT L  T  ++E I   VDV    ++      ++       +   A  +F +
Sbjct  40   SSISGSGRYTLLHLTNYADETISVAVDVTNVYIMGYLAGDVSYFFNEASATEAAKFVFKD  99

Query  303  SKLSINPDHRFAFATLSKSASWLRKEFSSDVESTIAAVRALSATTVSSGQADLTNLFRLA  124
            +K  +   +   +  L  +A  +R+     + +  +A+  L   T SS  + L  L +  
Sbjct  100  AKKKVTLPYSGNYERLQTAAGKIRENIPLGLPALDSAITTLYYYTASSAASALLVLIQST  159

Query  123  AHEAKKSRAQNRI  85
            A  A+    + +I
Sbjct  160  AESARYKFIEQQI  172


>gi|12644214|sp|P33185|RIP1_BRYDI  Ribosome-inactivating protein bryodin I precursor (rRNA N-glycosidase) 
(BD1)
 gi|17028176|gb|AAL34334.1|L42298_1  ribosome-inactivating protein [Bryonia dioica]
Length=290

 Score = 31.6 bits (70),  Expect = 1.8
 Identities = 29/133 (21%), Positives = 57/133 (42%), Gaps = 5/133 (3%)
 Frame = -3

Query  468  ASVGGTGRYT-LKPTRKSNEDIIFCVDVDAESLVEMKTAGIT----RLDSIRQAILLFVN  304
            +S+ G+GRYT L  T  ++E I   VDV    ++      ++       +   A  +F +
Sbjct  62   SSISGSGRYTLLHLTNYADETISVAVDVTNVYIMGYLAGDVSYFFNEASATEAAKFVFKD  121

Query  303  SKLSINPDHRFAFATLSKSASWLRKEFSSDVESTIAAVRALSATTVSSGQADLTNLFRLA  124
            +K  +   +   +  L  +A  +R+     + +  +A+  L   T SS  + L  L +  
Sbjct  122  AKKKVTLPYSGNYERLQTAAGKIRENIPLGLPALDSAITTLYYYTASSAASALLVLIQST  181

Query  123  AHEAKKSRAQNRI  85
            A  A+    + +I
Sbjct  182  AESARYKFIEQQI  194


>gi|15236271|ref|NP_192234.1|  DNA binding / transcription factor [Arabidopsis thaliana]
 gi|4262153|gb|AAD14453.1|  putative DNA-binding protein [Arabidopsis thaliana]
 gi|7270195|emb|CAB77810.1|  putative DNA-binding protein [Arabidopsis thaliana]
 gi|20152542|emb|CAD29664.1|  homeodomain protein 14 [Arabidopsis thaliana]
 gi|111074212|gb|ABH04479.1|  At4g03250 [Arabidopsis thaliana]
Length=476

 Score = 30.4 bits (67),  Expect = 4.1
 Identities = 15/40 (37%), Positives = 23/40 (57%), Gaps = 2/40 (5%)
 Frame = +1

Query  229  FSKPRGGFGQSGESE--AVIGVDREFGVDEEKDGLPYGIQ  342
            ++KP G     GESE  A+  V R+ G   ++DG P G++
Sbjct  153  YNKPSGYLKVKGESENFAITAVKRQLGRQYQEDGPPLGVE  192


>gi|15218851|ref|NP_171853.1|  unknown protein [Arabidopsis thaliana]
 gi|9280645|gb|AAF86514.1|AC002560_7  F21B7.16 [Arabidopsis thaliana]
 gi|91805735|gb|ABE65596.1|  pentatricopeptide repeat-containing protein [Arabidopsis thaliana]
Length=609

 Score = 29.3 bits (64),  Expect = 9.1
 Identities = 21/56 (37%), Positives = 27/56 (48%), Gaps = 3/56 (5%)
 Frame = +1

Query  169  GSSRECPNSSNGGLNITAELFSKPRGGFGQSGESEAVIGVDREFGVDEEKDGLPYG  336
            GS RE     NG     +  +S   GG+ Q+GE E  I + RE    EEKD   +G
Sbjct  314  GSVREARQVFNGMSKKNSVSWSALLGGYCQNGEHEKAIEIFREM---EEKDLYCFG  366

  Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding 
environmental samples
    Posted date:  Jan 15, 2007  4:53 AM
  Number of letters in database: 101,054,579
  Number of sequences in database:  283,349
Lambda     K      H
   0.318    0.134    0.401 
Gapped
Lambda     K      H
   0.267   0.0410    0.140 
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 283349
Number of Hits to DB: 383
Number of extensions: 6
Number of successful extensions: 0
Number of sequences better than 10: 0
Number of HSP's better than 10 without gapping: 0
Number of HSP's gapped: 0
Number of HSP's successfully gapped: 0
Length of query: 542
Length of database: 101054579
Length adjustment: 106
Effective length of query: 436
Effective length of database: 71019585
Effective search space: 5255449290
Effective search space used: 5255449290
T: 12
A: 40
X1: 16 (7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (20.4 bits)
S2: 64 (29.3 bits)