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SNP
OF RICE
SNP Indel_Type | 5_flanking seq | 3_flanking seq | Cutter enzyme | Primer Sequences | GC % | Annealing temp | Product size | Product after SNP cutter | |
Contg3:
T-A,A-C,A-C,C-G,A-G,A-C,C-A,G-T,G-T,G-A,C-A,G-A | CGTGGTCTGGAA | AGTTGACTACTGCCA | BsrFI
MspI
HpaII
BsrDI | FOR | CAGTAGGGGTGTGTA | 55.00 | 60.02 | 915 | 912,461 |
REV | CACTTCCAGACCACGTCCTT | 55.00 | 60.15 | ||||||
contg11:
T indel, C-T, T-C, C-T, A-C, G-A, T-G, G-T, C-G, T-C, A-T, C-A, A-C, T-A, C-T, G-C, C-G, T-C, A-T, T-A, C-T, A-C, C-A, A-C, G-A, A-G, T-A, A-T, C-A, T-C, C-T, G-C, A-G, C-A, A-C, T-A,
T indel
| CCGATTGAAG | GGTACGAGGTT | PspGI
StyD4I
BssKI | FOR | CCCCTGCCCGATGGTCCGCC | 80.00 | 70.00 | 1261 | 733,672 |
REV | CAGTGCGGATTTCGCTGAAA | 50.00 | 60.00 | ||||||
Contg17:
C-T, C-T
| - | CGATCGATCG | BsaHI | FOR | GGCGCTCTAAGGAATCTGAC | 55.00 | 59.03 | 671 | 63,638 |
REV | TTGTCCAGAGATGTTGTTGTCA | 40.91 | 59.19 | ||||||
Contg19:
C-G | TACCATGCTTATT | TGAAGAAAGGGTG | - | FOR | GAAAGGGTGGACAAAGTTCG | 50.00 | 59.57 | 188 | - |
REV | CTTCCCTCCCCTGGAACTGT | 60.00 | 62.50 | ||||||
contg 20:
A-C, G-A, T-C, G-A, T-C, T-C,A-T, C-G, G-A, G-A, C-T, A-G, G-A, C-T, A-C, T-C, T-C, T-C, T-C, T-C, G-A, G-A, A-G, G-A, C-G, C-A, A-T, A-T, T-G, T-A, A-T, G-T, A-T, T-G, G-A, A-G, G-A, T-G, C-T, T-A, C-T, A-T, G-T, A-T, G-A, T-G, A-G, A-C, T-A, T-A | AAATTGGCTTTG | AAGATGGCTTAC | FOR | TCCATACCACCTGTGGAACA | 50.00 | 59.81 | 1080 | ||
REV | AGCTTGAAAGGCTCAAAGC | 47.40 | 55.20 | ||||||
