LOCUS AW324525 1448 bp mRNA linear EST 25-SEP-2000 DEFINITION AB010 Basidiome and primordium cDNA libraries Agaricus bisporus cDNA 5' similar to Glycine rich protein, mRNA sequence. ACCESSION AW324525 VERSION AW324525.1 GI:10281753 KEYWORDS EST. SOURCE Agaricus bisporus ORGANISM Agaricus bisporus Eukaryota; Fungi; Basidiomycota; Hymenomycetes; Homobasidiomycetes; Agaricales; Agaricaceae; Agaricus. REFERENCE 1 (bases 1 to 1448) AUTHORS Ospina-Giraldo,M.D., Collopy,P.D., Romaine,C.P. and Royse,D.J. TITLE Classification of sequences expressed during the primordial and basidiome stages of the cultivated mushroom Agaricus bisporus JOURNAL Fungal Genet. Biol. 29 (2), 81-94 (2000) PUBMED 10919377 COMMENT Contact: Manuel D. Ospina-Giraldo Mushroom Research Laboratory, Department of Plant Pathology The Pennsylvania State University 305 Buckhout, University Park, PA 16802, USA Tel: 8148633073 Fax: 8148637217 Email: mxo11@psu.edu Seq primer: T7. FEATURES Location/Qualifiers source 1..1448 /organism="Agaricus bisporus" /mol_type="mRNA" /strain="Sylvan-130" /db_xref="taxon:5341" /clone_lib="Basidiome and primordium cDNA libraries" /note="Vector: pBluescript II SK (+); Site_1: SalI; Site_2: NotI" ORIGIN 1 cttccttgct gccttcatcg aggactccag tcgctgaagt gatcaatgga acgacataag 61 gtgccagaat ggtgtaaaca tatgcattca aagcattcgt cagctcgtct atgagatcgc 121 tcaaaccagg gaccatgtcc atcttctgag taatgtcttt cacaatgctg tcgcgccatt 181 tcaagacatc tatcagatta cgttgaactt caggaggtgc gatatcgttg ggatcaaagt 241 tgtatgcttt ggactgctgt tgcatttgat cgccttggtt tactttgtca tcgtttccgc 301 ccactggaag cttgccaata atgtctttga aagcgctgat attcgattct tccccttgag 361 aggcttcgtt catcttggtg gtaagttcag tgacactggc ctggctgagg tggtctccag 421 cctccccgag tacggaatgc aaaaagtctg cgccaccaaa ggtcccagta acgagcgggg 481 ggcaaggacc attaggtgtc acgtcggaga taacgtcatc gtcgacccac gcgtccgskg 541 acgcgtggcg ycgtatctct ctccctcctc tccctcctca ctttcctcgt ctcccatccc 601 agcctcgcct gtgtctaaca tgaaaacatt cacaaaaagt atagcttact ctccgactac 661 cgcggcaccc atcaccacta ttgactacac ccatggccaa ttccatcggc ctcgtccgag 721 tatcgttacg tcgtcacgcc ccattccaac tccagctcgt tctagttcta gccctggata 781 ctctacgcct ccgcttactc cggatgatgg cagctacagc agcagtggca gcattgatag 841 tattggactc aattcgccca ttcctactgc agttcatcaa aaacaaaagc cagggggtgg 901 cgtgcggtcc aaggatgcac tggaatatct catgactctc ttccctaagg atggattaaa 961 ggcgttaccc cacgctaaaa gtgtttcttt gactgcacct gctcttggcg cggactttga 1021 aggggtcgtt cttgaattac ccgggaaacc caagacgctt tacattgacg gcaagaatgc 1081 ggcttcggtc agtctccgtg aaagcgtcgt tgcgttgctg gatctagcag ccgataactt 1141 ggattgcggt gccctcgtca ttgttttgga aaggtcggct cctaatctgg gcgagctact 1201 gcattccttg atgtatgtgg gtggctcggt tgttacgaag ccaccttttt tcgtcgatcc 1261 ggcttttatt cttgttggtc tggaaatata aaggcactag gcaaggtaag atggtttttg 1321 tttaaatgaa aggggtggag tggttaggta gagagatcgg tctcttgagc aattaaagtc 1381 gttttggttt ttttttcttt gtatatacat tcaaatagta acatatgcga ctccatgtgt 1441 aaaaaaag //