LOCUS AW324531 928 bp mRNA linear EST 25-SEP-2000 DEFINITION AB050 Basidiome and primordium cDNA libraries Agaricus bisporus cDNA 5' similar to NADP-dependent isocitrate dehydrogenase, mRNA sequence. ACCESSION AW324531 VERSION AW324531.1 GI:10281759 KEYWORDS EST. SOURCE Agaricus bisporus ORGANISM Agaricus bisporus Eukaryota; Fungi; Basidiomycota; Hymenomycetes; Homobasidiomycetes; Agaricales; Agaricaceae; Agaricus. REFERENCE 1 (bases 1 to 928) AUTHORS Ospina-Giraldo,M.D., Collopy,P.D., Romaine,C.P. and Royse,D.J. TITLE Classification of sequences expressed during the primordial and basidiome stages of the cultivated mushroom Agaricus bisporus JOURNAL Fungal Genet. Biol. 29 (2), 81-94 (2000) PUBMED 10919377 COMMENT Contact: Manuel D. Ospina-Giraldo Mushroom Research Laboratory, Department of Plant Pathology The Pennsylvania State University 305 Buckhout, University Park, PA 16802, USA Tel: 8148633073 Fax: 8148637217 Email: mxo11@psu.edu Seq primer: T7. FEATURES Location/Qualifiers source 1..928 /organism="Agaricus bisporus" /mol_type="mRNA" /strain="Sylvan-130" /db_xref="taxon:5341" /clone_lib="Basidiome and primordium cDNA libraries" /note="Vector: pBluescript II SK (+); Site_1: SalI; Site_2: NotI" ORIGIN 1 tcgacccacg cgtccggtgt agaagagttc aaactgaagg agatgtggaa gagtccaaat 61 ggcactattc gtaacatcct cggaggaact gtcttccgtg aacctatcat tctgcagcgt 121 attccccgac ccatcccagg atgggtgaag cctatttgta ttggtcgtca tgctttcggt 181 gatcagtacc gctcgacgga ctasgtcgac ccacgcgtcc gggaaagctc caactggttt 241 tcacccctaa ggatggctct caaccagttt ccatgaacgt gttcgatttc gaaggacctg 301 gtgttgctat gagcatgtac aatactgacg aatccatcac tgggtttgcg cacgcttctt 361 tcaaaatggc catttccaag aaaatgccct tgttcatgtc tacgaaaaac accatcctga 421 agagatatga tggtcgcttc aaagacatct tccaggagat ctatgaatca acctacaaac 481 ctcaatttga agccgctggc atctattacg aacatcgttt gatcgacgac atggtcgccc 541 aagccattaa atcctcgggt ggtttcgttt gggcctgcaa aaactacgat ggcgacgtcc 601 aatccgacat tctcgctcag ggtttcggtt ccctcggtat gatgacttca gaactcttga 661 cacccgacgg tcaaatcatc gaatccgaag ctgctcatgg aacagttaca aggcattatc 721 gggaatggca aaagggtaat gagacgtcga ctaatcctgt tgcatccatt tttgcttgga 781 cgagagggtt gttgcatagg gcgaagcttg ataagaatga gcctttgaaa cagttttgtt 841 tggacttgga gagcgcttgt gttgaggtta ttgataaggg atggtgtgat gaccaaggat 901 cttgcgattg cgattcatgg agtcaaga //