LOCUS AW324532 822 bp mRNA linear EST 25-SEP-2000 DEFINITION AB058 Basidiome and primordium cDNA libraries Agaricus bisporus cDNA 5' similar to Malate dehydrogenase, mRNA sequence. ACCESSION AW324532 VERSION AW324532.1 GI:10281760 KEYWORDS EST. SOURCE Agaricus bisporus ORGANISM Agaricus bisporus Eukaryota; Fungi; Basidiomycota; Hymenomycetes; Homobasidiomycetes; Agaricales; Agaricaceae; Agaricus. REFERENCE 1 (bases 1 to 822) AUTHORS Ospina-Giraldo,M.D., Collopy,P.D., Romaine,C.P. and Royse,D.J. TITLE Classification of sequences expressed during the primordial and basidiome stages of the cultivated mushroom Agaricus bisporus JOURNAL Fungal Genet. Biol. 29 (2), 81-94 (2000) PUBMED 10919377 COMMENT Contact: Manuel D. Ospina-Giraldo Mushroom Research Laboratory, Department of Plant Pathology The Pennsylvania State University 305 Buckhout, University Park, PA 16802, USA Tel: 8148633073 Fax: 8148637217 Email: mxo11@psu.edu Seq primer: T7. FEATURES Location/Qualifiers source 1..822 /organism="Agaricus bisporus" /mol_type="mRNA" /strain="Sylvan-130" /db_xref="taxon:5341" /clone_lib="Basidiome and primordium cDNA libraries" /note="Vector: pBluescript II SK (+); Site_1: SalI; Site_2: NotI" ORIGIN 1 agtaaggggt gggatgattg agaaaggagg ggcacgatgg tgacaccact gtgaccgcca 61 acaacaggaa cgacgacttc cgtcgctaaa gacagattac cgtgcttctc agcgacaaag 121 gttgatgaac ggacaacgtc gagggttgta acaccgaaca gacgtttcgg gtcgtaaaca 181 ccatgcttct tgagaacttc ggcaacgata ggaactgttg agtttacagg gtttgaaata 241 acgagaacga aagccttggg agaatacgta gcgatccccg tcgcgagatc acgtacaatt 301 ccagcgttaa ttttgaaaag gtcatctctt gtcattccag gcttacgagg aacgccagcg 361 ggaattacca caacatcagc gcccttgagc gttttggaga ggccgtcgtt atcaggcaaa 421 ttaccctcca ccttggcggg tgtggaaata tgcgcgagat cggcagcaac acctggggtg 481 ttgacgatgc caccagcagc accaagaacg acagccttga ccattgtccc acgcgtccgc 541 gatggaykcc gkgggctsgc agtcgtctca atcgcagcca aacggcgtgc atgacactct 601 ccagacaggc gcccgtcctg ccatctccat ccccgtcatc aaccaccgcc ccgcagagcc 661 cagatctccc ccgcccccca ttcctgcaga gcccgccgcc tccccgccgc ccccgccgcc 721 ctccacggaa ccctccccgc cgccaccccc gcccccgagg tcgcccactc tgcttcctga 781 gaaaaaagag cccatatcct tctctctggt accgaaacca cg //