LOCUS AW324533 518 bp mRNA linear EST 25-SEP-2000 DEFINITION AB060 Basidiome and primordium cDNA libraries Agaricus bisporus cDNA 5', mRNA sequence. ACCESSION AW324533 VERSION AW324533.1 GI:10281761 KEYWORDS EST. SOURCE Agaricus bisporus ORGANISM Agaricus bisporus Eukaryota; Fungi; Basidiomycota; Hymenomycetes; Homobasidiomycetes; Agaricales; Agaricaceae; Agaricus. REFERENCE 1 (bases 1 to 518) AUTHORS Ospina-Giraldo,M.D., Collopy,P.D., Romaine,C.P. and Royse,D.J. TITLE Classification of sequences expressed during the primordial and basidiome stages of the cultivated mushroom Agaricus bisporus JOURNAL Fungal Genet. Biol. 29 (2), 81-94 (2000) PUBMED 10919377 COMMENT Contact: Manuel D. Ospina-Giraldo Mushroom Research Laboratory, Department of Plant Pathology The Pennsylvania State University 305 Buckhout, University Park, PA 16802, USA Tel: 8148633073 Fax: 8148637217 Email: mxo11@psu.edu Seq primer: T7. FEATURES Location/Qualifiers source 1..518 /organism="Agaricus bisporus" /mol_type="mRNA" /strain="Sylvan-130" /db_xref="taxon:5341" /clone_lib="Basidiome and primordium cDNA libraries" /note="Vector: pBluescript II SK (+); Site_1: SalI; Site_2: NotI" ORIGIN 1 tcsaccswcg cgtcckattc aacccactct cctttaagct ctatgactct tcaacgcaaa 61 tctcacaaac tcatcaagcg ccagctcggc cccttggatc cccccgccga casccccgac 121 gctcctcctg acccccccgc tgcagatcct cccgcaggtg gcggcaatgg tggtggaaat 181 ggtggcggcg gtactcctcc tggccgccct ggcgacgacg acggcccacc ycgaggtggt 241 ggcaatggcg gtgacggtcc cactcggtcg gcttctgagt cttccagcac ctcgtctacc 301 cgccctgccc gtccgacaga accatccact acctcctcga cgccgatcac cacttctcgt 361 acaacaccct ctaccacctc tacatctacc tctacctcta ccaccacttc ctctaccctg 421 acctcgacct ctactacccc gtccaccact tccactactc caacttctac ttccacccgc 481 acctttattc tcacggctcc tacccagacc aacaccat //