LOCUS AW324536 1356 bp mRNA linear EST 25-SEP-2000 DEFINITION AB090 Basidiome and primordium cDNA libraries Agaricus bisporus cDNA 5', mRNA sequence. ACCESSION AW324536 VERSION AW324536.1 GI:10281764 KEYWORDS EST. SOURCE Agaricus bisporus ORGANISM Agaricus bisporus Eukaryota; Fungi; Basidiomycota; Hymenomycetes; Homobasidiomycetes; Agaricales; Agaricaceae; Agaricus. REFERENCE 1 (bases 1 to 1356) AUTHORS Ospina-Giraldo,M.D., Collopy,P.D., Romaine,C.P. and Royse,D.J. TITLE Classification of sequences expressed during the primordial and basidiome stages of the cultivated mushroom Agaricus bisporus JOURNAL Fungal Genet. Biol. 29 (2), 81-94 (2000) PUBMED 10919377 COMMENT Contact: Manuel D. Ospina-Giraldo Mushroom Research Laboratory, Department of Plant Pathology The Pennsylvania State University 305 Buckhout, University Park, PA 16802, USA Tel: 8148633073 Fax: 8148637217 Email: mxo11@psu.edu Seq primer: T7. FEATURES Location/Qualifiers source 1..1356 /organism="Agaricus bisporus" /mol_type="mRNA" /strain="Sylvan-130" /db_xref="taxon:5341" /clone_lib="Basidiome and primordium cDNA libraries" /note="Vector: pBluescript II SK (+); Site_1: SalI; Site_2: NotI" ORIGIN 1 tcgacccacg cgtccgatga actgtaagcc agatcatcgg agtctaattc ctaactcaat 61 tggaagaagg ctcttgtcaa cacgtgtctg ttaggatctt agtagtcagg agagtgttgt 121 aaggaaggct tgcgacggtg atgcgcccat cgactaatac gggaaaccga accgtcgtag 181 ggcttaatgc aatgttcagg gtgaaaaaga acatagcatc atctattagc gmyysacsmg 241 tyykgcgcga ctggaggaca ggagcggata agatccgtac cctacctact tcactttaaa 301 tattcttgat gaaactatcc gacatggatt aaaacacgga caggtggcgt cgttagcgtc 361 acagtgggct gatcgtcccg gggacaggag cgggattcgt tctcgtaccc cagctaggtt 421 caaaaataat cggttttatc gcccgatgga caagagcggc ttatccctcg cttaatgtaa 481 ttaggagtgg gggttagggt cgtacccaac caacaacaaa actttcgact ttttgtctgc 541 gtaagaagtc gtgcaagtgg gagtcgagtt aattctcgcg tatggagacc aatgcacgtg 601 ttgactacgt taaggacgaa aatgaatctc acaatgccta tccaggctta aaatggaaat 661 aggttgttcc cgtcgttcgg ttctgccaac acgacgtgga atgaccggtt cttcctgaac 721 acgaaaaaga acgctgaggc accatcagtt ttactagccg accggggtcg acgccgcatg 781 tgaacgcaac tccctggtaa cgtagactaa ttgatatagg cccaagaggc gcaatgaggt 841 taaaggatgt tttcaaagtc aatcccttta ggagaggggt gggtcatttc caatcatgaa 901 cggctgatgg gcgattgtag gcagtgttct aggatttgat ttttgttccc gtcggagtaa 961 gtcgactggg gttacactcc ctggtaacgt taactctctg gcaaaggggc actgcttaat 1021 tgcagacacc gtggtcgctt ggagaagctc ctccgctaag cacaggaccc atgggagtag 1081 tgatgtgtag aaaacaaaag tggtatccat cgtggaatcc ggcgtgaaac tcttccctta 1141 actgggtgat ttggagtggt cggagtaaac tcgaaagcga gtctcttttt ggggaagcat 1201 cgttgatgac ttgaggcttc tgtygtatcg ttcgtgaata ttgcacgttg ggtcggctca 1261 tcagctggtc tagctttgtg atgtccgcgg gcgtacagta ttagcagcat tggaaggggt 1321 atgtggtgga gcaatctgct gctaacctat tcaact //