ID   MTDH_AGABI     STANDARD;      PRT;   261 AA.
AC   O93868;
DT   16-OCT-2001 (Rel. 40, Created)
DT   16-OCT-2001 (Rel. 40, Last sequence update)
DT   25-OCT-2004 (Rel. 45, Last annotation update)
DE   NADP-dependent mannitol dehydrogenase (EC 1.1.1.138) (MtDH) (Mannitol
DE   2-dehydrogenase [NADP+]).
GN   Name=mtdH;
OS   Agaricus bisporus (Common mushroom).
OC   Eukaryota; Fungi; Basidiomycota; Hymenomycetes; Homobasidiomycetes;
OC   Agaricales; Agaricaceae; Agaricus.
OX   NCBI_TaxID=5341;
RN   [1]
RP   NUCLEOTIDE SEQUENCE, PROTEIN SEQUENCE OF 1-19 AND 210-229, AND
RP   CHARACTERIZATION.
RC   STRAIN=Horst U1;
RX   MEDLINE=99054901; PubMed=9835550;
RA   Stoop J.M.H., Mooibroek H.;
RT   "Cloning and characterization of NADP-mannitol dehydrogenase cDNA from
RT   the button mushroom, Agaricus bisporus, and its expression in response
RT   to NaCl stress.";
RL   Appl. Environ. Microbiol. 64:4689-4696(1998).
RN   [2]
RP   PRELIMINARY CRYSTALLOGRAPHIC ANALYSIS.
RX   PubMed=11320313; DOI=10.1107/S0907444901002542;
RA   Sassoon J., Hoerer S., Stoop J., Mooibroek H., Baumann U.;
RT   "Crystallization and preliminary crystallographic analysis of mannitol
RT   dehydrogenase (MtDH) from the common mushroom Agaricus bisporus.";
RL   Acta Crystallogr. D 57:711-713(2001).
RN   [3]
RP   X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS) IN COMPLEX WITH NADP, AND
RP   HOMOTETRAMERIZATION.
RX   PubMed=11335726; DOI=10.1074/jbc.M102850200;
RA   Horer S., Stoop J., Mooibroek H., Baumann U., Sassoon J.;
RT   "The crystallographic structure of the mannitol 2-dehydrogenase NADP+
RT   binary complex from Agaricus bisporus.";
RL   J. Biol. Chem. 276:27555-27561(2001).
CC   -!- CATALYTIC ACTIVITY: D-mannitol + NADP(+) = D-fructose + NADPH.
CC   -!- SUBUNIT: Homotetramer.
CC   -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases
CC       (SDR) family.
CC   --------------------------------------------------------------------------
CC   This Swiss-Prot entry is copyright. It is produced through a collaboration
CC   between  the Swiss Institute of Bioinformatics  and the  EMBL outstation -
CC   the European Bioinformatics Institute.  There are no  restrictions on  its
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CC   --------------------------------------------------------------------------
DR   EMBL; AF053764; AAC79985.1; -.
DR   PDB; 1H5Q; X-ray; A/B/C/D/E/F/G/H/I/J/K/L=1-261.
DR   InterPro; IPR002198; ADH_short.
DR   InterPro; IPR002347; Adh_short_C2.
DR   Pfam; PF00106; adh_short; 1.
DR   PRINTS; PR00081; GDHRDH.
DR   PRINTS; PR00080; SDRFAMILY.
KW   3D-structure; Direct protein sequencing; NADP; Oxidoreductase.
FT   INIT_MET      0      0
FT   NP_BIND      18     43       NADP.
FT   NP_BIND     149    173       NADP.
FT   ACT_SITE    168    168       Proton acceptor.
FT   BINDING     148    148       Substrate.
FT   STRAND        4      6
FT   TURN          9     10
FT   STRAND       11     16
FT   TURN         17     19
FT   HELIX        21     32
FT   TURN         33     34
FT   STRAND       35     41
FT   TURN         45     46
FT   HELIX        47     58
FT   TURN         59     59
FT   STRAND       62     66
FT   TURN         69     70
FT   HELIX        72     85
FT   STRAND       89     94
FT   HELIX       104    106
FT   HELIX       109    119
FT   TURN        120    120
FT   HELIX       121    137
FT   TURN        138    138
FT   STRAND      141    146
FT   HELIX       149    151
FT   TURN        152    152
FT   STRAND      156    158
FT   TURN        159    160
FT   STRAND      161    162
FT   HELIX       166    186
FT   HELIX       187    189
FT   TURN        190    190
FT   STRAND      191    198
FT   STRAND      201    201
FT   HELIX       204    208
FT   HELIX       211    219
FT   TURN        220    220
FT   TURN        222    223
FT   STRAND      227    227
FT   HELIX       229    232
FT   HELIX       233    240
FT   HELIX       242    244
FT   TURN        245    246
FT   STRAND      251    254
FT   TURN        256    257
FT   HELIX       258    261
SQ   SEQUENCE   261 AA;  27888 MW;  D90C541720D647DC CRC64;
     APGFTISFVN KTIIVTGGNR GIGLAFTRAV AAAGANVAVI YRSAKDAVEV TEKVGKEFGV
     KTKAYQCDVS NTDIVTKTIQ QIDADLGAIS GLIANAGVSV VKPATELTHE DFKFVYDVNV
     FGVFNTCRAV AKLWLQKQQK GSIVVTSSMS SQIINQSSLN GSLTQVFYNS SKAACSNLVK
     GLAAEWASAG IRVNALSPGY VNTDQTAHMD KKIRDHQASN IPLNRFAQPE EMTGQAILLL
     SDHATYMTGG EYFIDGGQLI W
//