ID   KPYK_AGABI     STANDARD;      PRT;   532 AA.
AC   O94122; Q9UVX2;
DT   16-OCT-2001 (Rel. 40, Created)
DT   16-OCT-2001 (Rel. 40, Last sequence update)
DT   25-OCT-2004 (Rel. 45, Last annotation update)
DE   Pyruvate kinase (EC 2.7.1.40) (PK).
GN   Name=pkiA;
OS   Agaricus bisporus (Common mushroom).
OC   Eukaryota; Fungi; Basidiomycota; Hymenomycetes; Homobasidiomycetes;
OC   Agaricales; Agaricaceae; Agaricus.
OX   NCBI_TaxID=5341;
RN   [1]
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=Horst H39;
RA   Schaap P.J., Mueller Y., van Griensven L.J.L.D., Visser J.;
RT   "Sequence of the Agaricus bisporus pkiA gene.";
RL   Submitted (APR-1996) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE OF 3-532.
RC   STRAIN=Horst U1;
RA   De Groot P.W.J., Visser J., van Griensven L.J.L.D., Schaap P.J.;
RT   "Biochemical and molecular aspects of growth on fruiting of the edible
RT   mushroom Agaricus bisporus.";
RL   Mycol. Res. 102:1297-1308(1998).
CC   -!- CATALYTIC ACTIVITY: ATP + pyruvate = ADP + phosphoenolpyruvate.
CC   -!- COFACTOR: Requires magnesium and potassium (By similarity).
CC   -!- PATHWAY: Glycolysis; final step.
CC   -!- SUBUNIT: Homotetramer (By similarity).
CC   -!- SIMILARITY: Belongs to the pyruvate kinase family.
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CC   This Swiss-Prot entry is copyright. It is produced through a collaboration
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CC   the European Bioinformatics Institute.  There are no  restrictions on  its
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DR   EMBL; X97579; CAA66194.1; -.
DR   EMBL; X91106; CAA62560.1; -.
DR   HSSP; P14178; 1E0T.
DR   InterPro; IPR011037; PK_B_barrel_like.
DR   InterPro; IPR001697; Pyruvate_kinase.
DR   Pfam; PF00224; PK; 1.
DR   Pfam; PF02887; PK_C; 1.
DR   PRINTS; PR01050; PYRUVTKNASE.
DR   ProDom; PD001009; Pyruvate_kinase; 2.
DR   TIGRFAMs; TIGR01064; pyruv_kin; 1.
DR   PROSITE; PS00110; PYRUVATE_KINASE; 1.
KW   Glycolysis; Kinase; Magnesium; Transferase.
FT   ACT_SITE    254    254       By similarity.
FT   METAL       256    256       Magnesium (Potential).
FT   METAL       277    277       Magnesium (Potential).
FT   METAL       278    278       Magnesium (Potential).
FT   BINDING     351    351       ADP (Potential).
SQ   SEQUENCE   532 AA;  58249 MW;  3D569B9A2639CDE6 CRC64;
     MYPVDGIRSQ IEWNTTLNVS DAPVPTETTK YHRKTAIIAT IGPKINTVEK LTDVRLAGVN
     IVRMNFSHGT HEYHQSVIDN TRQMIKNDPH GRPVAIALDT KGPEIRTGQT RDGNDYPIKA
     GDEFIVTTDP KYSDICDNKV LFVDYANLAS VTAPGKLIYI DDGIISLLIL SIDGMNLHVR
     ALNNGTLSSR KGVNLPKTDV DLPPLSEKDK DDLRFGIRNG VDMIFASFIR RGEDVRQIRE
     VLGPDGASIK IIVKIENEQG VANFDEILKE ADGVMVARGD LGIEIPASQV FLAQKMMIAK
     CNIVGKPVIV ATQMLESMTY NPRPTRAEVS DVANAVLDGS DCVMLSGETA KGSYPVQSVL
     MMAETCLLAE TAICYPPLYD DLRAVQARPT ETAETVAIAA VAAAAEQDAK ALLVLSTSGE
     TARLVSKYRP KIPIITVTRN EQTARQIHLH RGCYPFWYPE PRGVQNHQWQ TDVDNRIRFG
     LRNALALNVI QPGASIIAVQ GWKGGLGHTN TLRILTVPTD PADLELQPLG SL
//