ID   PUT2_AGABI     STANDARD;      PRT;   546 AA.
AC   P78568;
DT   01-NOV-1997 (Rel. 35, Created)
DT   01-NOV-1997 (Rel. 35, Last sequence update)
DT   25-OCT-2004 (Rel. 45, Last annotation update)
DE   Delta-1-pyrroline-5-carboxylate dehydrogenase (EC 1.5.1.12) (P5C
DE   dehydrogenase).
GN   Name=pruA;
OS   Agaricus bisporus (Common mushroom).
OC   Eukaryota; Fungi; Basidiomycota; Hymenomycetes; Homobasidiomycetes;
OC   Agaricales; Agaricaceae; Agaricus.
OX   NCBI_TaxID=5341;
RN   [1]
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=Horst H39;
RX   MEDLINE=97133945; PubMed=8979339;
RA   Schaap P.J., Mueller Y., Sonnenberg A.S.M., van Griensven L.J.L.D.,
RA   Visser J.;
RT   "The Agaricus bisporus pruA gene encodes a cytosolic delta 1-
RT   pyrroline-5-carboxylate dehydrogenase which is expressed in fruit
RT   bodies but not in gill tissue.";
RL   Appl. Environ. Microbiol. 63:57-62(1997).
CC   -!- CATALYTIC ACTIVITY: 1-pyrroline-5-carboxylate + NAD(+) + H(2)O =
CC       L-glutamate + NADH.
CC   -!- PATHWAY: Conversion from proline to glutamate; second step.
CC   -!- SUBCELLULAR LOCATION: Cytoplasmic.
CC   -!- SIMILARITY: Belongs to the aldehyde dehydrogenase family.
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DR   EMBL; X95584; CAA64836.1; -.
DR   InterPro; IPR002086; Aldehyde_dehydr.
DR   InterPro; IPR005931; D1pyr5carbox1.
DR   Pfam; PF00171; Aldedh; 1.
DR   TIGRFAMs; TIGR01236; D1pyr5carbox1; 1.
DR   PROSITE; PS00070; ALDEHYDE_DEHYDR_CYS; 1.
DR   PROSITE; PS00687; ALDEHYDE_DEHYDR_GLU; FALSE_NEG.
KW   NAD; Oxidoreductase; Proline metabolism.
FT   NP_BIND     279    284       NAD (ADP part) (By similarity).
FT   ACT_SITE    297    297       By similarity.
FT   ACT_SITE    331    331       By similarity.
SQ   SEQUENCE   546 AA;  59757 MW;  6B795E09AC242945 CRC64;
     MATAQLATFK IPPVSNEPML SYAPGSPERA GLQAALAEMQ SQLPFEVPCI INGQEVRTNN
     IQKQPMPHDH ARHLCTFHEG SPELVEKATC GALQAKDGWE TMPWNDRAAI FLKAADLASG
     KYRYKLMAAT MLGQGKNTWQ AEIDAAAELA DFFRFGVSYV EELYAQQPPK NAPGCWNRTE
     YRPLEGFVLA VSPFNFTAIG GNLPGSPALV GNVVVWKPAP AATYSNYLVF KILSEAGVPP
     GVIQFIPGGA EIVQAAIQSP NFRSLHFTGS TNVFKSLWKD ISSNLDKYKV YPRIVGETGG
     KNWHVIHKSA EVRNAVLQSV RGAFEYQGQK CSALSRLYVS RSVWENGFKT QYLEEIAKIK
     VGPCLDWNNY MGPVIGRRAY DNITGFIKKA KEEGGEVLIG GSGDDSKGFF IQPTVILTKV
     PRSTTMVGEI FGPVVTAYVF EDSDYEKTLE LIDTTSIYGL TGAIFASERQ ALLTATNRSR
     NAAGNIYYNE KCTGAVVGQQ PFGGARGSGT NDKAGSISIF YRFVSARSIK ENFVGLEDFH
     YPSNLV
//