LOCUS       AY501890                1000 bp    DNA     linear   PLN 13-OCT-2004
DEFINITION  Aspergillus flavus isolate Chapman2M truncated polyketide synthase
            (pksA) gene, complete cds.
ACCESSION   AY501890
VERSION     AY501890.1  GI:46361463
KEYWORDS    .
SOURCE      Aspergillus flavus
  ORGANISM  Aspergillus flavus
            Eukaryota; Fungi; Ascomycota; Pezizomycotina; Eurotiomycetes;
            Eurotiales; Trichocomaceae; mitosporic Trichocomaceae; Aspergillus.
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Ehrlich,K.C. and Cotty,P.J.
  TITLE     An isolate of Aspergillus flavus used to reduce aflatoxin
            contamination in cottonseed has a defective polyketide synthase
            gene
  JOURNAL   Appl. Microbiol. Biotechnol. 65 (4), 473-478 (2004)
REFERENCE   2  (bases 1 to 1000)
  AUTHORS   Ehrlich,K.C. and Cotty,P.J.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-DEC-2003) Food and Feed Safety, Southern Regional
            Research Center/USDA, PO Box 19687, New Orleans, LA 70179, USA
FEATURES             Location/Qualifiers
     source          1..1000
                     /organism="Aspergillus flavus"
                     /mol_type="genomic DNA"
                     /isolate="Chapman2M"
                     /db_xref="taxon:5059"
                     /note="VCG CH10"
     gene            <190..>1000
                     /gene="pksA"
     mRNA            join(<190..476,542..836,901..>1000)
                     /gene="pksA"
                     /product="truncated polyketide synthase"
     CDS             join(190..476,542..782)
                     /gene="pksA"
                     /inference="non-experimental evidence, no additional
                     details recorded"
                     /note="PKSA; aflatoxin biosynthesis gene cluster"
                     /codon_start=1
                     /product="truncated polyketide synthase"
                     /protein_id="AAS89278.1"
                     /db_xref="GI:46361464"
                     /translation="MAQSRQLFLFGDQTADFVPKLRSLLSVQDSPILAAFLDQSHYVV
                     RAQMLQSMNTVDHKLARTADLRQMVQKYVDGKLTPAFRTALVCLCQLGCFIREYEESG
                     NMYPQPSDSYVLGFCMGSLAAVAVSCSRSLSELLPIAVQTVLIAFRLGLCALEMRDRV
                     DGCSDDRGDPWSTIV"
     variation       782
                     /gene="pksA"
                     /note="results in premature stop codon"
ORIGIN      
        1 actggtccgc ggctgtctgg cctcttggta acctggacgg cagagctgag ggctccgata
       61 cgactcagtt catcggtcaa cgacgaagta tctcgaagtg tagttttcaa atactgatat
      121 agcttcatat agctcctcgg ggcagacctg cttccatcca gtttcaacaa tctcataccc
      181 tatcccacaa tggctcaatc aaggcaactc tttctcttcg gcgatcagac agcggatttt
      241 gttcccaagc tccgcagttt actatccgtc caggacagcc ctattctagc cgcctttctt
      301 gaccagtccc actatgtcgt gcgagcccag atgctgcaga gcatgaacac ggttgatcac
      361 aagttggctc gaaccgctga cctgcgccaa atggtccaga agtatgtcga cggcaaactg
      421 accccggcat ttcgaaccgc tctagtgtgc ctctgccagt tgggatgctt catccggtac
      481 gttccctctt tttgtgctgc cttagatcgg tccctttact ttcttctaaa aatcgtacta
      541 gggaatatga ggaatctggc aacatgtacc cacagcccag tgacagctac gtgctgggat
      601 tctgcatggg ttccttggcc gctgtggcgg taagctgcag tcgctccttg tcagagctgc
      661 tgcctatcgc tgtacaaact gtgttgattg ccttccgcct cggtctttgc gccctggaga
      721 tgcgggatcg ggtggatggg tgtagcgatg atcgaggtga cccttggtct accattgttt
      781 gaggtctgga tccccagcaa gctcgtgatc agattgaagt gttctgtcag accacagtga
      841 gtgcttctat atctttcggt acccaggaaa agatgttgtc ctaacgatgt aatgcaatag
      901 aacgtacctc agacaaggcg cccgtggatc agctgcatct ctaagaatgc catcacccta
      961 agtggcagtc catccacttt gagggcgttc tgtgcgatgc
//