ID   AY501898   standard; genomic DNA; FUN; 1000 BP.
XX
AC   AY501898;
XX
SV   AY501898.1
XX
DT   13-OCT-2004 (Rel. 81, Created)
DT   13-OCT-2004 (Rel. 81, Last updated, Version 1)
XX
DE   Aspergillus flavus isolate Bayside2e truncated polyketide synthase (pksA)
DE   gene, complete cds.
XX
KW   .
XX
OS   Aspergillus flavus
OC   Eukaryota; Fungi; Ascomycota; Pezizomycotina; Eurotiomycetes; Eurotiales;
OC   Trichocomaceae; mitosporic Trichocomaceae; Aspergillus.
XX
RN   [1]
RP   1-1000
RA   Ehrlich K.C., Cotty P.J.;
RT   "An isolate of Aspergillus flavus used to reduce aflatoxin contamination in
RT   cottonseed has a defective polyketide synthase gene";
RL   Appl. Microbiol. Biotechnol. 65(4):473-478(2004).
XX
RN   [2]
RP   1-1000
RA   Ehrlich K.C., Cotty P.J.;
RT   ;
RL   Submitted (16-DEC-2003) to the EMBL/GenBank/DDBJ databases.
RL   Food and Feed Safety, Southern Regional Research Center/USDA, PO Box 19687,
RL   New Orleans, LA 70179, USA
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1000
FT                   /db_xref="taxon:5059"
FT                   /mol_type="genomic DNA"
FT                   /note="VCG BS07"
FT                   /organism="Aspergillus flavus"
FT                   /isolate="Bayside2e"
FT   gene            <190..>1000
FT                   /gene="pksA"
FT   mRNA            join(<190..476,542..836,901..>1000)
FT                   /gene="pksA"
FT                   /product="truncated polyketide synthase"
FT   CDS             join(190..476,542..782)
FT                   /codon_start=1
FT                   /evidence=NOT_EXPERIMENTAL
FT                   /note="PKSA; aflatoxin biosynthesis gene cluster"
FT                   /gene="pksA"
FT                   /product="truncated polyketide synthase"
FT                   /protein_id="AAS89286.1"
FT                   /translation="MAQSRQLFLFGDQTADFVPKLRSLLSVQDSPILAAFLDQSHYVVR
FT                   AQMLQSMNTVDHKLARTADLRQMVQKYVDGKLTPAFRTALVCLCQLGCFIREYEESGNM
FT                   YPQPSDSYVLGFCMGSLAAVAVSCSRSLSELLPIAVQTVLIAFRLGLCALEMRDRVDGC
FT                   SDDRGDPWSTIV"
FT   variation       782
FT                   /note="results in premature stop codon"
FT                   /gene="pksA"
XX
SQ   Sequence 1000 BP; 211 A; 273 C; 250 G; 266 T; 0 other;
     actggtccgc ggctgtctgg cctcttggta acctggacgg cagagctgag ggctccgata        60
     cgactcagtt catcggtcaa cgacgaagta tctcgaagtg tagttttcaa atactgatat       120
     agcttcatat agctcctcgg ggcagacctg cttccatcca gtttcaacaa tctcataccc       180
     tatcccacaa tggctcaatc aaggcaactc tttctcttcg gcgatcagac agcggatttt       240
     gttcccaagc tccgcagttt actatccgtc caggacagcc ctattctagc cgcctttctt       300
     gaccagtccc actatgtcgt gcgagcccag atgctgcaga gcatgaacac ggttgatcac       360
     aagttggctc gaaccgctga cctgcgccaa atggtccaga agtatgtcga cggcaaactg       420
     accccggcat ttcgaaccgc tctagtgtgc ctctgccagt tgggatgctt catccggtac       480
     gttccctctt tttgtgctgc cttagatcgg tccctttact ttcttctaaa aatcgtacta       540
     gggaatatga ggaatctggc aacatgtacc cacagcccag tgacagctac gtgctgggat       600
     tctgcatggg ttccttggcc gctgtggcgg taagctgcag tcgctccttg tcagagctgc       660
     tgcctatcgc tgtacaaact gtgttgattg ccttccgcct cggtctttgc gccctggaga       720
     tgcgggatcg ggtggatggg tgtagcgatg atcgaggtga cccttggtct accattgttt       780
     gaggtctgga tccccagcaa gctcgtgatc agattgaagt gttctgtcag accacagtga       840
     gtgcttctat atctttcggt acccaggaaa agatgttgtc ctaacgatgt aatgcaatag       900
     aacgtacctc agacaaggcg cccgtggatc agctgcatct ctaagaatgc catcacccta       960
     agtggcagtc catccacttt gagggcgttc tgtgcgatgc                            1000
//