ID AY501899 standard; genomic DNA; FUN; 1000 BP. XX AC AY501899; XX SV AY501899.1 XX DT 13-OCT-2004 (Rel. 81, Created) DT 13-OCT-2004 (Rel. 81, Last updated, Version 1) XX DE Aspergillus flavus isolate 91060a polyketide synthase (pksA) gene, partial DE cds. XX KW . XX OS Aspergillus flavus OC Eukaryota; Fungi; Ascomycota; Pezizomycotina; Eurotiomycetes; Eurotiales; OC Trichocomaceae; mitosporic Trichocomaceae; Aspergillus. XX RN [1] RP 1-1000 RA Ehrlich K.C., Cotty P.J.; RT "An isolate of Aspergillus flavus used to reduce aflatoxin contamination in RT cottonseed has a defective polyketide synthase gene"; RL Appl. Microbiol. Biotechnol. 65(4):473-478(2004). XX RN [2] RP 1-1000 RA Ehrlich K.C., Cotty P.J.; RT ; RL Submitted (16-DEC-2003) to the EMBL/GenBank/DDBJ databases. RL Food and Feed Safety, Southern Regional Research Center/USDA, PO Box 19687, RL New Orleans, LA 70179, USA XX FH Key Location/Qualifiers FH FT source 1..1000 FT /db_xref="taxon:5059" FT /mol_type="genomic DNA" FT /note="VCG OD02" FT /organism="Aspergillus flavus" FT /isolate="91060a" FT gene <190..>1000 FT /gene="pksA" FT mRNA join(<190..476,542..836,901..>1000) FT /gene="pksA" FT /product="polyketide synthase" FT CDS join(190..476,542..836,901..>1000) FT /codon_start=1 FT /note="PKSA; aflatoxin biosynthesis gene cluster" FT /gene="pksA" FT /product="polyketide synthase" FT /protein_id="AAS89287.1" FT /translation="MAQSRQLFLFGDQTADFVPKLRSLLSVQDSPILAAFLDQSHYVVR FT AQMLQSMNTIDHKLARTADLRQMVQKYVDGKLTPAFRTALVCLCQLGCFIREYEESGNM FT YPQPSDSYVLGFCMGSLAAVAVSCSRSLSELLPIAVQTVLIAFRLGLCALEMRDRVDGC FT SDDRGDPWSTIVWGLDPQQARDQIEVFCQTTNAPQTRRPWISCISKNAITLSGSPSTLR FT AFCEM" XX SQ Sequence 1000 BP; 211 A; 273 C; 251 G; 265 T; 0 other; actggtccgc ggctgtctgg cctcttggta acctggacgg cagagctgag ggctccgata 60 cgactcagtt catcggtcaa cgacgaagta tctcgaagtg tagttttcaa atactgatat 120 agcttcatat agctcctcgg ggcagacctg cttccatcca gtttcaacaa tctcataccc 180 tatcccacaa tggctcaatc aaggcaactc tttctcttcg gcgatcagac agcggatttt 240 gttcccaagc tccgcagttt actatccgtc caggacagcc ctattctagc cgcctttctt 300 gaccagtccc actatgtcgt gcgagcccag atgctgcaga gcatgaacac gattgatcac 360 aagttggctc gaaccgctga cctgcgccaa atggtccaga agtatgtcga cggcaaactg 420 accccggcat ttcgaaccgc tctagtgtgc ctctgccagt tgggatgctt catccggtac 480 gttccctctt tttgtgctgc cttagatcgg tccctttact ttcttctaaa aatcgtacta 540 gggaatatga ggaatctggc aacatgtacc cacagcccag tgacagctac gtgctgggat 600 tctgcatggg ttccttggcc gctgtggcgg taagctgcag tcgctccttg tcagagctgc 660 tgcctatcgc tgtacaaact gtgttgattg ccttccgcct cggtctttgc gccctggaga 720 tgcgggatcg ggtggatggg tgtagcgatg atcgaggtga cccttggtct accattgttt 780 ggggtctgga tccccagcaa gctcgtgatc agattgaagt gttctgtcag accacagtga 840 gtgcttctat atctttcggt acccaggaaa agatgttgtc ctaacgatgt aatgcaatag 900 aacgcacctc agacgaggcg cccgtggatc agctgcatct ctaagaatgc catcacccta 960 agtggcagtc catccacttt gagggcgttc tgtgagatgc 1000 //