ID   CP53_ASPNG     STANDARD;      PRT;   517 AA.
AC   P17549;
DT   01-AUG-1990 (Rel. 15, Created)
DT   01-AUG-1990 (Rel. 15, Last sequence update)
DT   25-JAN-2005 (Rel. 46, Last annotation update)
DE   Benzoate 4-monooxygenase (EC 1.14.13.12) (Benzoate-para-hydroxylase)
DE   (Cytochrome P450 53) (BpH).
GN   Name=bphA; Synonyms=cyp53, cyp53A1;
OS   Aspergillus niger.
OC   Eukaryota; Fungi; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiales; Trichocomaceae; mitosporic Trichocomaceae; Aspergillus.
OX   NCBI_TaxID=5061;
RN   [1]
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=ATCC 1055 / DSM 2061;
RX   MEDLINE=91066829; PubMed=2250647;
RA   van Gorcom R.F.M., Boschloo J.G., Kuijvenhoven A., Lange J.,
RA   van Vark A.J., Bos C.J., van Balken J.A.M., Pouwels P.H.,
RA   van den Hondel C.A.M.J.J.;
RT   "Isolation and molecular characterisation of the benzoate-para-
RT   hydroxylase gene (bphA) of Aspergillus niger: a member of a new gene
RT   family of the cytochrome P450 superfamily.";
RL   Mol. Gen. Genet. 223:192-197(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE.
RA   Higgins U.M., Darby R.M., Maddison A., Lamb D.C., Draper J.;
RT   "Yeast heterologous expression of a benzoate 4-hydroxylase (CYP53)
RT   from Aspergillus niger.";
RL   Submitted (SEP-2001) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   CHARACTERIZATION.
RC   STRAIN=T18.5;
RX   MEDLINE=21478854; PubMed=11594739; DOI=10.1006/abbi.2001.2534;
RA   Faber B.W., van Gorcom R.F.M., Duine J.A.;
RT   "Purification and characterization of benzoate-para-hydroxylase, a
RT   cytochrome P450 (CYP53A1), from Aspergillus niger.";
RL   Arch. Biochem. Biophys. 394:245-254(2001).
CC   -!- FUNCTION: Responsible for cytochrome P450 dependent benzoate
CC       hydroxylation in microsomes; requires cprA as the mediator in
CC       electron donation from NADPH.
CC   -!- CATALYTIC ACTIVITY: Benzoate + NADPH + O(2) = 4-hydroxybenzoate +
CC       NADP(+) + H(2)O.
CC   -!- SIMILARITY: Belongs to the cytochrome P450 family.
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DR   EMBL; X52521; CAA36753.1; -.
DR   EMBL; AJ347748; CAC69995.1; -.
DR   PIR; S12015; S12015.
DR   HSSP; P14779; 1JPZ.
DR   GO; GO:0005792; C:microsome; IDA.
DR   GO; GO:0018664; F:benzoate 4-monooxygenase activity; IDA.
DR   GO; GO:0006118; P:electron transport; IDA.
DR   InterPro; IPR001128; Cytochrome_P450.
DR   InterPro; IPR002401; EP450I.
DR   Pfam; PF00067; p450; 1.
DR   PIRSF; PIRSF000054; Pisatin_de-mtase; 1.
DR   PRINTS; PR00463; EP450I.
DR   PRINTS; PR00385; P450.
DR   PROSITE; PS00086; CYTOCHROME_P450; 1.
KW   Electron transport; Heme; Monooxygenase; NADP; Oxidoreductase.
FT   METAL       461    461       Iron (heme axial ligand) (By similarity).
SQ   SEQUENCE   517 AA;  58016 MW;  63FFBFDF703EC30B CRC64;
     MLALLLSPYG AYLGLALLVL YYLLPYLKRA HLRDIPAPGL AAFTNFWLLL QTRRGHRFVV
     VDNAHKKYGK LVRIAPRHTS IADDGAIQAV YGHGNGFLKS DFYDAFVSIH RGLFNTRDRA
     EHTRKRKTVS HTFSMKSIGQ FEQYIHGNIE LFVKQWNRMA DTQRNPKTGF ASLDALNWFN
     YLAFDIIGDL AFGAPFGMLD KGKDFAEMRK TPDSPPSYVQ AVEVLNRRGE VSATLGCYPA
     LKPFAKYLPD SFFRDGIQAV EDLAGIAVAR VNERLRPEVM ANNTRVDLLA RLMEGKDSNG
     EKLGRAELTA EALTQLIAGS DTTSNTSCAI LYWCMRTPGV IEKLHKALDE AIPQDVDVPT
     HAMVKDIPYL QWVIWETMRI HSTSAMGLPR EIPAGNPPVT ISGHTFYPGD VVSVPSYTIH
     RSKEIWGPDA EQFVPERWDP ARLTPRQKAA FIPFSTGPRA CVGRNVAEME LLVICGTVFR
     LFEFEMQQEG PMETREGFLR KPLGLQVGMK RRQPGSA
//