ID   ACEA_NEUCR     STANDARD;      PRT;   548 AA.
AC   P28299; Q7RVC2; Q9HEI3;
DT   01-DEC-1992 (Rel. 24, Created)
DT   16-OCT-2001 (Rel. 40, Last sequence update)
DT   05-JUL-2004 (Rel. 44, Last annotation update)
DE   Isocitrate lyase (EC 4.1.3.1) (Isocitrase) (Isocitratase) (ICL).
GN   Name=acu-3; ORFNames=2E4.10, NCU04230.1;
OS   Neurospora crassa.
OC   Eukaryota; Fungi; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Sordariomycetidae; Sordariales; Sordariaceae; Neurospora.
OX   NCBI_TaxID=5141;
RN   [1]
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=74A;
RX   MEDLINE=92136435; PubMed=1531185;
RA   Gainey L.D.S., Connerton I.F., Lewis E.H., Turner G., Ballance D.J.;
RT   "Characterization of the glyoxysomal isocitrate lyase genes of
RT   Aspergillus nidulans (acuD) and Neurospora crassa (acu-3).";
RL   Curr. Genet. 21:43-47(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=74-OR23-1A / FGSC 987;
RX   MEDLINE=22542210; PubMed=12655011; DOI=10.1093/nar/gkg293;
RA   Mannhaupt G., Montrone C., Haase D., Mewes H.-W., Aign V.,
RA   Hoheisel J.D., Fartmann B., Nyakatura G., Kempken F., Maier J.,
RA   Schulte U.;
RT   "What's in the genome of a filamentous fungus? Analysis of the
RT   Neurospora genome sequence.";
RL   Nucleic Acids Res. 31:1944-1954(2003).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=74-OR23-1A / FGSC 987;
RX   PubMed=12712197; DOI=10.1038/nature01554;
RA   Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D.,
RA   Jaffe D., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B.,
RA   Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M.,
RA   Qui D., Ianakiev P., Bell-Pedersen D., Nelson M.A.,
RA   Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L.,
RA   Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C.,
RA   Marcotte E., Greenberg D., Roy A., Foley K., Naylor J.,
RA   Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M.,
RA   Mauceli E., Bielke C., Rudd S., Frishman D., Krystofova S.,
RA   Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C.,
RA   Macino G., Catcheside D., Li W., Pratt R.J., Osmani S.A.,
RA   DeSouza C.P., Glass L., Orbach M.J., Berglund J.A., Voelker R.,
RA   Yarden O., Plamann M., Seiler S., Dunlap J., Radford A., Aramayo R.,
RA   Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M.,
RA   Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.;
RT   "The genome sequence of the filamentous fungus Neurospora crassa.";
RL   Nature 422:859-868(2003).
CC   -!- CATALYTIC ACTIVITY: Isocitrate = succinate + glyoxylate.
CC   -!- PATHWAY: Glyoxylate bypass; first step.
CC   -!- SUBUNIT: Homotetramer (By similarity).
CC   -!- SUBCELLULAR LOCATION: Glyoxysomal.
CC   -!- INDUCTION: By acetate.
CC   -!- SIMILARITY: Belongs to the isocitrate lyase family.
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DR   EMBL; X62697; CAA44573.1; -.
DR   EMBL; AL451022; CAC18302.1; -.
DR   EMBL; AABX01000286; EAA31618.1; -.
DR   PIR; S26858; S26858.
DR   HSSP; P28298; 1DQU.
DR   InterPro; IPR006254; Isocit_lyase.
DR   InterPro; IPR000918; Isocit_lyase_ph.
DR   Pfam; PF00463; ICL; 1.
DR   ProDom; PD001857; Isocit_lyase_ph; 1.
DR   TIGRFAMs; TIGR01346; isocit_lyase; 1.
DR   PROSITE; PS00161; ISOCITRATE_LYASE; 1.
KW   Glyoxylate bypass; Glyoxysome; Lyase; Tricarboxylic acid cycle.
FT   ACT_SITE    215    215       Probable.
FT   CONFLICT    132    132       V -> C (in Ref. 1).
FT   CONFLICT    286    286       D -> H (in Ref. 1).
FT   CONFLICT    422    424       VHA -> PR (in Ref. 1).
SQ   SEQUENCE   548 AA;  61214 MW;  4C3414CE0794D44C CRC64;
     MAANNMVNPA VDPALEDELF AKEVEEVKKW WSDSRWRQTK RPFTAEQIVS KRGNLKIEYA
     SNAQAKKLWK ILEDRFAKRD ASYTYGCLEP TMVTQMAKYL DTVYVSGWQS SSTASSSDEP
     GPDLADYPYT TVPNKVGHLF MAQLFHDRKQ RQERLSVPKD QREKLANIDY LRPIVADADT
     GHGGLTAVMK LTKLFIEKGA AGIHIEDQAP GTKKCGHMAG KVLVPIQEHI NRLVAIRAQA
     DIMGSDLLCI ARTDAEAATL ITTTIDPRDH AFILGCTNPD LEPLADLMMK AEAEGKTGAQ
     LQAIEDDWLA KADLKRFDEA VLDVIAKGKF SNAKDLAAKY QAAVKGKQIS NREARAIARQ
     LLGQEIFFDW ESPRTREGYY RLKGGCDCSI NRAISYAPYC DAIWMESKLP DYAQAEEFAK
     GVHAVWPEQK LAYNLSPSFN WKTAMGRDDQ ETYIRRLAKL GYCWQFITLA GLHTTALISD
     QFAKAYSKIG MRAYGELVQE PEIDNGVDVV KHQKWSGATY VDELQKMVTG GVSSTAAMGK
     GVTEDQFH
//