ID AF200742 standard; genomic DNA; PRO; 6480 BP. XX AC AF200742; XX SV AF200742.1 XX DT 03-DEC-2000 (Rel. 66, Created) DT 09-JUN-2001 (Rel. 68, Last updated, Version 2) XX DE Azoarcus sp. BH72 Mo-nitrogenase operon, complete sequence. XX KW . XX OS Azoarcus sp. BH72 OC Bacteria; Proteobacteria; Betaproteobacteria; Rhodocyclales; OC Rhodocyclaceae; Azoarcus. XX RN [1] RP 1-6480 RX MEDLINE; 21264388. RX PUBMED; 11371540. RA Egener T., Martin D.E., Sarkar A., Reinhold-Hurek B.; RT "Role of a Ferredoxin Gene Cotranscribed with the nifHDK Operon in N(2) RT Fixation and Nitrogenase 'Switch-Off' of Azoarcus sp. Strain BH72"; RL J. Bacteriol. 183(12):3752-3760(2001). XX RN [2] RP 1-6480 RA Egener T., Reinhold-Hurek B.; RT ; RL Submitted (01-NOV-1999) to the EMBL/GenBank/DDBJ databases. RL AG Symbiose, Max-Planck-Institut for Terrestrische Mikrobiologie, RL Karl-von-Frisch-Str., Marburg D-35043, Germany XX FH Key Location/Qualifiers FH FT source 1..6480 FT /db_xref="taxon:62928" FT /mol_type="genomic DNA" FT /organism="Azoarcus sp. BH72" FT /strain="BH72" FT promoter 522..534 FT /note="putative -12/-24 promoter sequence (sigma 54)" FT /gene="Mo-nitrogenase operon" FT CDS 620..1513 FT /codon_start=1 FT /db_xref="GOA:Q9F0V9" FT /db_xref="UniProt/TrEMBL:Q9F0V9" FT /note="NifH; iron protein" FT /transl_table=11 FT /gene="nifH" FT /product="dinitrogenase reductase subunit" FT /protein_id="AAG35586.1" FT /translation="MAKLRQCAIYGKGGIGKSTTTQNLVAALAEAGKKVMIVGCDPKAD FT STRLILHSKAQTTVMHLAAEAGSVEDLELDDVLSVGFGGVKCVESGGPEPGVGCAGRGV FT ITAINFLEEEGAYDDELDFVFYDVLGDVVCGGFAMPIRENKAQEIYIVCSGEMMAMYAA FT NNIAKGIVKYANSGGVRLGGLICNSRNTDREDELIEALAAAMGTQMIHFVPRDNAVQHA FT EIRRMTVIEYDPTHKQADQYRQLAQKVLNNKMLVIPTPIEMEQLEALLMEFGIMEQEDE FT SIVGQTAAELAAGAAA" FT CDS 1803..3110 FT /codon_start=1 FT /db_xref="GOA:Q9F0V8" FT /db_xref="UniProt/TrEMBL:Q9F0V8" FT /note="NifD" FT /transl_table=11 FT /gene="nifD" FT /product="Mo-protein dinitrogenase subunit" FT /protein_id="AAG35587.1" FT /translation="MTIRGCAYAGSRGVVWGPVKDMIHISHGPVGCGQYSRGGRRNYYV FT GTTGVNTFAEMNFTSDFQEKDIVFGGDKKLAQAMAEIEQLFPLNKGISVQSECPIGLIG FT DDIEAVSKKAAAQYNKVVVPVRCEGFRGVSQSLGHHIANDAIRDYVLSNRDGKEFESTP FT YDVTIIGDYNIGGDAWASRIFLEEMGLRVIAQWSGDGTIAEMENTPKAKLNLLHCYRSM FT NYISRHMEEKYGIPWMEYNFFGPTKIEESLRRIAAFFDDSIKAKCEEVIAKYKPMMQAV FT IDKYKPRLQGKRVMLYIGGLRPRHVIGAYEDLGMEVVGTGYEFAHNDDYDRTIQEMGNA FT TLLYDDVTGFELEEFVKRIKPDLVGSGIKEKYIYQKMGIPFRQMHSWDYSGPYHGYDGF FT AIFARDMDMTLNNPCWGKQTPPWKKTEATEDVIAAA" FT CDS 3214..4779 FT /codon_start=1 FT /db_xref="GOA:Q9F0V7" FT /db_xref="UniProt/TrEMBL:Q9F0V7" FT /note="NifK" FT /transl_table=11 FT /gene="nifK" FT /product="Mo-protein dinitrogenase reductase subunit" FT /protein_id="AAG35588.1" FT /translation="MQAVDDIKPCYPLFRDDDYKENLKRKQDLYEERVPADKIAETFEW FT TTSKEYQELNFKREALTINPAKACQPLGAVLCSLGFKNTMPYVHGSQGCVAYFRTYFNR FT HFKEPVSCVSDSMTEDAAVFGGQKNMFDGLENAKAIYKPDMIAVSTTCMAEVIGDDLNA FT FINNAKKAGHVPQEYPVPFAHTPSFVGSHTTGWDNMWEGIARFFTLNKMEGKVVGSNGK FT LNFVPGFETYLGNYRVIKRMMGQMGVDATVLSDPSEVLDTPSDGEFRMYSGGTTMDEMS FT DAPNALNTIVLQPWQLEKSKKFVETVWNHDVPKLNIPMGLDWTDEFLMKVSEITGKAIP FT EELALERGRLVDMMQDSHTWLHGKKFALYGDPDFVLGLTKILLELGAEPIHILSNNANK FT RWGKAVQKVLDGYKHGANCKVYVGNDLWHMRSLCFTEKPDFLIGNSYGKYIQRDTVFKG FT KEFEVPLIRLGFPIFDRHHLHRMTTLGYEGAMYVLTTLVNAVLERLDDETRGMGTDYNY FT DLVR" FT CDS 5137..5340 FT /codon_start=1 FT /db_xref="GOA:Q9F0V6" FT /db_xref="UniProt/TrEMBL:Q9F0V6" FT /note="FdxN" FT /transl_table=11 FT /gene="fdxN" FT /product="electron carrier protein ferredoxin N" FT /protein_id="AAG35589.1" FT /translation="MALKIVLDECTSCGDCKPVCPTKSITDKGGIFKINKDTCTECEGD FT FDEPQCLAVCPAGDACIVPLAA" FT CDS 5423..6145 FT /codon_start=1 FT /db_xref="UniProt/TrEMBL:Q9F0V5" FT /note="similar to Azotobacter vinelandii NifY nitrogenase FT maturation protein" FT /transl_table=11 FT /gene="nifY" FT /product="NifY protein" FT /protein_id="AAG35590.1" FT /translation="MNTASPTGSPVTREAALRIAMAARALPGVEVVAFVRALGDRLGLP FT VTDEKLARVTVEDLKALLQGDEIVDPGVESSALKAAVRFVWGEGLDDGQPTPDADVPAA FT EGALKVAVASNTGELLDGHFGSCARFLVYQVSEEGVWLIDVRSTEGTDDADDRNVARAA FT LINDCHLAYMQSIGGPAAAKIVRAGIHPVKFPVGGEARQVLAQLQGTLKRPPPWLAKVL FT GREAPSLAQFTSDEEAEA" FT CDS 6142..6414 FT /codon_start=1 FT /db_xref="UniProt/TrEMBL:Q9F0V4" FT /note="ORF1; similar to Azotobacter vinelandii ORF1" FT /transl_table=11 FT /product="unknown" FT /protein_id="AAG35591.1" FT /translation="MINDELLGTVAGLVKEQGASEQLVSALRVRWPNLRFTYCSDDDMP FT PRLSPALAGEGFNLYLIANGDHCLALTKDPAVAIGVVVAEVMADE" XX SQ Sequence 6480 BP; 1304 A; 2058 C; 1982 G; 1136 T; 0 other; gactctagag gatcgcgccc tcgcgcccct cggagtcgaa cagccagccg cgcagcaggc 60 gcaggtagtc gagcttgctg cggttgatgc gggcggtgtc ggacaaaccg agcgccggcg 120 cgtcgtcgag caggaaatgg gcggtcatgt aggactggaa cagcgctgcc cgctcggcgg 180 cgttctccag ctcgtccagg cgatcgaaca gcggccggtg gagcggcagc actccatcga 240 gttcgagccc cgcggcccca gctggaaggc caggctggcg agtgcatccg gcggcaggtt 300 gcagcggttg atcgcccgcc gggcctgcgc gggcacggtc gtacgcaaca gcgcggggtc 360 gatgtcgggt gtctccacag gcgtgtagtt ggccttacaa ggggtggggg cattgtcgca 420 acaacgacga ctaccggcgt cttccgcgtt ccccgaggga ttctgctgct cgttgaatag 480 ttcttttgaa acagtcattt gtgttgattg tgtggtgact ggcacggtga ttgccatgga 540 gtggtcgtgg gcgccgggca atgttcctcc gggcccagga cacccggtag tttttttctt 600 caacgaggag agtttcaaca tggcaaagct gcgtcaatgc gccatttatg gcaagggcgg 660 tatcggcaag tcgaccacca cccagaacct ggttgcggcc ctggccgaag ccggcaagaa 720 ggtcatgatc gtcggttgcg acccgaaggc tgactccacc cgtctgatcc tccacagcaa 780 ggcccagacc accgtgatgc acctggccgc tgaagccggc tcggttgaag acctcgaact 840 cgacgacgtc ctgtcggtcg gcttcggtgg cgtgaagtgc gtcgagtccg gcggtccgga 900 acccggcgtc ggctgcgccg gccgtggcgt tatcaccgcc atcaacttcc tggaagaaga 960 aggcgcctac gacgacgaac tcgacttcgt gttctacgac gtgctgggcg acgtggtgtg 1020 tggtggcttc gcgatgccga ttcgcgaaaa caaggcccag gaaatctaca tcgtctgctc 1080 gggcgaaatg atggccatgt acgccgccaa caacatcgcc aagggcatcg tgaagtacgc 1140 caactccggt ggcgtgcgcc tcggcggcct gatctgcaac agccgcaaca ccgaccgcga 1200 agacgaactg atcgaagcgc tggccgccgc catgggcacc cagatgatcc acttcgtgcc 1260 gcgtgacaac gccgtgcagc acgccgaaat ccgccgcatg accgtcatcg agtacgaccc 1320 gacccacaag caggccgacc agtaccgtca gctcgcccag aaggtgctga acaacaagat 1380 gctcgtcatc ccgaccccga tcgagatgga acagctcgaa gccctgctga tggaattcgg 1440 catcatggaa caggaagacg agtccatcgt cggccagacc gctgccgagc ttgccgccgg 1500 cgccgctgcc taagtcagcc tcgttcgagc aagtctctac cgagtgcatc cggccgcggt 1560 tcgccgtggc cggacttgaa tcccgtcttg agtgtcgtgc acagattggt gccggcatga 1620 aaaggagaag caagatgtcg aacctgaccc gtgaagaaac cgacgccctc atccaggagg 1680 tgctggaggt ttatcccgag aaggccaaga aggatcgcgc caagcacctg gcggtcaacg 1740 atcagagcat cacccagtcg aagaagtgca tcacctccaa ccgcaagtcc ctgccgggcg 1800 tcatgaccat ccgtggttgc gcctacgccg gttcgcgcgg cgtggtgtgg ggtccggtca 1860 aggacatgat ccacatctcg cacggcccgg ttggctgtgg ccaatactcc cgtggcggcc 1920 gccgcaacta ctacgtcggc accaccggcg tgaacacctt cgccgaaatg aacttcacct 1980 cggacttcca ggagaaggac atcgttttcg gcggtgacaa gaagctcgcc caggccatgg 2040 ccgaaatcga gcagctgttc cccctgaaca agggtatctc ggtccagtcc gagtgcccga 2100 tcggcctgat cggcgacgac atcgaagccg tctccaagaa ggctgccgcg cagtacaaca 2160 aggttgtcgt gccggtccgc tgcgaaggct tccgtggcgt gtcccagtcc ctgggccacc 2220 acatcgccaa cgacgcgatc cgtgactacg tcctgtcgaa ccgcgacggc aaggaattcg 2280 aaagcacccc gtacgacgtc accatcatcg gcgactacaa catcggcggc gacgcctggg 2340 cttcccgtat cttcctcgaa gaaatgggcc tgcgcgtgat cgcccagtgg tccggtgacg 2400 gcaccatcgc cgaaatggag aacaccccga aggcgaagct gaacctcctc cactgctacc 2460 gctcgatgaa ctacatcagc cgtcacatgg aagagaagta cgggattccc tggatggagt 2520 acaacttctt cggcccgacc aagatcgaag aatcgctccg ccgcatcgcc gccttcttcg 2580 acgactcgat caaggccaag tgcgaggaag tcatcgccaa gtacaagccg atgatgcaag 2640 ccgtcatcga caagtacaag ccgcgcctcc agggcaagcg cgtcatgctc tacatcggtg 2700 gcctgcgtcc gcgtcacgtc atcggcgcgt acgaagacct cggcatggaa gtcgtgggca 2760 cgggttatga gttcgcccac aacgacgact acgaccgcac catccaggaa atgggcaatg 2820 cgaccctgct gtacgacgac gtcaccggct tcgagctgga agagttcgtc aagcgcatca 2880 agcccgacct cgtcggttcc ggcatcaagg aaaagtacat ctatcaaaag atgggcattc 2940 ccttccgtca gatgcacagc tgggactact ccggtccgta tcacggttat gacggtttcg 3000 cgatcttcgc gcgcgacatg gacatgaccc tgaacaaccc gtgctggggc aagcagaccc 3060 cgccgtggaa gaagaccgaa gcgaccgagg acgtgatagc tgccgcctga tcgaccttcc 3120 ggatggacct ggtgcgccgg ggtcggcgca ccaggtatcg aatgcactca agcccgtgtc 3180 cacagattgc tggccgggtt aaggagctga aaaatgcaag ccgtagacga tatcaagccg 3240 tgttacccgc tgttccgtga cgacgactac aaggaaaacc tgaagcgcaa gcaggacctg 3300 tacgaagagc gcgttccggc cgacaagatc gccgaaacct tcgaatggac cacctcgaag 3360 gaataccagg aactgaactt caagcgcgaa gcactcacca tcaaccccgc caaggcctgc 3420 cagccgctgg gcgcggtgct gtgctcgctg ggcttcaaga acacgatgcc ctacgtgcat 3480 ggttcgcagg gttgcgtggc ctacttccgc acctacttca accgtcactt caaggagccg 3540 gtgtcctgcg tgtccgactc catgacggaa gatgcggcgg tgttcggcgg ccagaagaac 3600 atgttcgacg gcctggaaaa cgccaaggcg atctacaagc cggacatgat cgcggtctcc 3660 accacctgca tggccgaggt gatcggtgac gacttgaacg ccttcatcaa caacgcgaag 3720 aaggccggtc acgttccgca ggaatacccg gttccgttcg cccacacccc gtccttcgtc 3780 ggcagccaca ccaccggctg ggacaacatg tgggaaggca tcgcgcgctt cttcacgctg 3840 aacaagatgg aaggcaaggt tgtcggcagc aacggcaagc tgaacttcgt gccgggcttc 3900 gaaacctacc tgggcaacta ccgcgtcatc aagcgcatga tgggtcagat gggtgtcgac 3960 gccaccgtgc tgtccgatcc gagcgaagtg ctggataccc cgtccgacgg cgaattccgc 4020 atgtactcgg gcggcaccac gatggacgag atgtccgatg cgccgaacgc gctcaacacc 4080 atcgtgctgc agccgtggca actcgagaag tccaagaagt tcgtggaaac cgtgtggaac 4140 cacgacgtgc ccaagctcaa catcccgatg ggcctggact ggaccgacga gttcctgatg 4200 aaggtctccg agatcaccgg caaggcgatt ccggaagagc tggcgctcga gcgtggccgc 4260 ctggtcgaca tgatgcagga ctcccacacc tggctgcacg gcaagaagtt cgccctgtac 4320 ggcgacccgg acttcgtgct cggcctgacc aagatcctgc tcgaactcgg cgcggaaccg 4380 atccacatcc tgtcgaacaa cgccaacaag cgttggggca aggcggtgca gaaggtgctc 4440 gacggctaca agcacggcgc caactgcaag gtgtatgtcg gcaacgacct gtggcacatg 4500 cgctcgctgt gcttcaccga gaagccggac ttcctgatcg gcaactcgta cggcaagtac 4560 atccagcgtg acacggtgtt caagggcaag gaattcgagg ttccgctgat ccgcctcggc 4620 ttcccgatct tcgaccgcca ccacctgcac cgcatgacca ccctgggcta cgaaggcgcg 4680 atgtatgtgc tgaccaccct ggtgaacgcc gtcctcgagc gtctggacga cgaaacccgc 4740 ggcatgggta ccgactacaa ctacgacctc gttcgctaat cagcgatcac gggacgggcg 4800 ccttcgcggc acccgtcccg ctttcacgtt tgcgaaccct gcatgcgggc gtcatcgtgt 4860 ccgcgcgcgt aattggagat gagttgtggc caatatcatg atccgcaaga ccgccagtgg 4920 tgggctttcg ttctacctgc ccaagcgcga cctcgaggat gacatcgtct ccatcgagtt 4980 cgaccagccc gacaagtggg gcggtgcgct caagctgctc aacggcggtg agtactacgt 5040 cgagccgctc gaagcgcctc ccaagctgcc gatcacggtg cgcgccaagc gtgtggatgc 5100 gcccgaataa gggcgcgttt tccaggagaa ggaatcatgg ctctgaagat tgtgctggac 5160 gaatgcacgt cctgtggtga ctgcaagccc gtctgcccga ccaagtcgat caccgacaag 5220 ggcggcattt tcaagatcaa caaggacacc tgtaccgagt gcgagggcga tttcgacgag 5280 ccccagtgcc tggcggtctg tcccgctggt gacgcctgta tcgtgccgct cgcggcctga 5340 tgcgaaaaag gagccgcgcg tgcgcggctc cctgtttttt gtagcccgcg tatcccacgc 5400 ccgaattccc atgaggctcg tcatgaatac cgctagcccg accggaagtc ccgtcacccg 5460 ggaagccgcc ctgcgtatcg cgatggcagc acgcgcgctg cccggtgtcg aggtcgtcgc 5520 cttcgtgcgc gcgctcggcg accgcctggg gctgcctgtc accgacgaaa agctcgcccg 5580 cgtcaccgtc gaggatctga aggccctgct gcagggcgac gagatcgtcg atccgggagt 5640 cgagtcgtcg gcgctcaagg ccgccgtgcg ttttgtctgg ggcgaaggcc tcgatgacgg 5700 ccaacccacc cccgatgccg acgtgcctgc cgccgagggc gcgttgaagg tggcggtggc 5760 ctcgaacacc ggcgaactgc tcgatggcca ctttggctcc tgcgcgcgtt tcctggttta 5820 tcaggtcagt gaggaaggcg tctggctgat cgacgtgcgc tccaccgagg gcaccgacga 5880 cgccgacgac cgcaatgtcg cgcgcgccgc cttgatcaac gattgtcatc tcgcctacat 5940 gcagtcgatc ggcggcccgg ccgcggccaa gatcgtgcgc gccggcattc acccggtgaa 6000 gttcccggtt ggcggcgaag cccgccaggt gcttgcacag ctgcagggca cgctgaagcg 6060 ccctccgccg tggctggcca aggtgctggg ccgtgaagcg cccagcctcg cgcagttcac 6120 ttccgacgag gaggccgaag catgatcaac gatgaactgc tgggcaccgt cgccgggctg 6180 gtgaaggagc agggcgccag cgagcagctg gtcagcgcgc tgcgggtgcg ctggcccaac 6240 ctgcgcttca cctattgttc cgacgacgac atgccgccgc gtctatcgcc ggcgctcgcc 6300 ggcgagggct tcaatctcta cctgatcgcc aacggcgacc attgcctcgc actgacaaag 6360 gatcccgcgg ttgcgatcgg cgtcgtggtt gccgaggtta tggccgacga ataagcgcga 6420 tttcgccttt tccagggccg ccgtccttca ggactgcggc ctttttgatg cttgccgttt 6480 //