ID   CO050526   standard; mRNA; EST; 587 BP.
XX
AC   CO050526;
XX
SV   CO050526.1
XX
DT   15-JUN-2004 (Rel. 80, Created)
DT   16-JUN-2004 (Rel. 80, Last updated, Version 2)
XX
DE   Le_mx0_15b12_SP6 Little Skate Multiple Tissues, Normalized Leucoraja
DE   erinacea cDNA clone Le_mx0_15b12 5' similar to emb|CAF21979.1|
DE   TPR-repeat-containing protei - Azoarcus sp. EbN1. Score = 33.9 bits (76),
DE   Expect = 2.1, mRNA sequence.
XX
KW   EST.
XX
OS   Leucoraja erinacea (little skate)
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Chondrichthyes;
OC   Elasmobranchii; Squalea; Hypnosqualea; Pristiorajea; Batoidea; Rajiformes;
OC   Rajidae; Leucoraja.
XX
RN   [1]
RP   1-587
RA   Towle D.W., Smith C.M.;
RT   "Expressed sequence tags in a normalized cDNA library prepared from
RT   multiple tissues of adult little skate, Leucoraja erinacea";
RL   Unpublished.
XX
DR   UNILIB; 35911; 15890.
XX
CC   Contact: David W. Towle
CC   Marine DNA Sequencing and Analysis Center
CC   Mount Desert Island Biological Laboratory
CC   Old Bar Harbor Road, Salsbury Cove, ME 04672 USA
CC   Tel: 207-288-9880 x474
CC   Fax: 207-288-2130
CC   Email: dtowle@mdibl.org
CC   Plate: 15  row: b  column: 12
CC   Seq primer: SP6
CC   High quality sequence stop: 469.
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..587
FT                   /db_xref="taxon:7782"
FT                   /db_xref="UNILIB:35911"
FT                   /mol_type="mRNA"
FT                   /note="Vector: pCMV Sport 6.1; Total RNA samples were
FT                   prepared individually from each tissue, checked for
FT                   quality, then pooled for construction and normalization of
FT                   cDNA library by Invitrogen. Plasmids were isolated and
FT                   inserts end-sequenced by the Marine DNA Sequencing and
FT                   Analysis Facility at Mount Desert Island Biological
FT                   Laboratory. Traces were processed for submission to dbEST
FT                   by trace2dbest software (Parkinson, Anthony and Blaxter,
FT                   unpublished software)."
FT                   /organism="Leucoraja erinacea"
FT                   /clone="Le_mx0_15b12"
FT                   /tissue_type="Liver, kidney, brain, testis, ovary, gill,
FT                   heart, spleen, rectal gland"
FT                   /dev_stage="Adult"
FT                   /clone_lib="Little Skate Multiple Tissues, Normalized"
XX
SQ   Sequence 587 BP; 179 A; 102 C; 129 G; 177 T; 0 other;
     agccagtgag ggtttgggtt aataaatcca aatgggtgat ggaaggtcca gaagggattg        60
     tgtgagagaa tgactagaaa cctggcccca aaagaacggg attaaactgg gcagattatc       120
     aatgtcattg cacaaatgtt gtttcactag tctggagggt cacccatccc ttctctccag       180
     agatgctgcc tgtcccactg agttactgcg gcattttgtg tccatcttca gtaaaacaat       240
     ggaatcgggg taaatgcttg aaagttcaga tcggcgaata aaataaagta ctctttccca       300
     tttcccaaaa tgaaatcatg tagataaaat gtatttagat aaaagtgctt gcagcgaatg       360
     cctggtctaa aattatagac tcagtacatg aagtaggact tgaactcgga aagagacaaa       420
     aagtgctgga gtaatacatt tgtctatttt gtaaaccagc atctgcattt tcttctgtct       480
     tgaaatcaga aggcagtcac tgggtagaat ctttgcctcc agtaaacaat gatttttgct       540
     tgtttttgaa ttttttaaat agtgtgtatt tattgctcca tgctaat                     587
//