ID   DUS_AZOBR      STANDARD;      PRT;   328 AA.
AC   P45672;
DT   01-NOV-1995 (Rel. 32, Created)
DT   01-NOV-1995 (Rel. 32, Last sequence update)
DT   05-JUL-2004 (Rel. 44, Last annotation update)
DE   Probable tRNA-dihydrouridine synthase (EC 1.-.-.-).
GN   Name=dus;
OS   Azospirillum brasilense.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales;
OC   Rhodospirillaceae; Azospirillum.
OX   NCBI_TaxID=192;
RN   [1]
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=Sp7 / ATCC 29145;
RX   MEDLINE=96002420; PubMed=7553451;
RA   Machado H.B., Yates M.G., Funayama S., Rigo L.U., Steffens M.B.R.,
RA   Souza E.M., Pedrosa F.O.;
RT   "The ntrBC genes of Azospirillum brasilense are part of a nifR3-like-
RT   ntrB-ntrC operon and are negatively regulated.";
RL   Can. J. Microbiol. 41:674-684(1995).
CC   -!- FUNCTION: Catalyzes the synthesis of dihydrouridine, a modified
CC       base found in the D-loop of most tRNAs (By similarity).
CC   -!- COFACTOR: FAD (By similarity).
CC   -!- SIMILARITY: Belongs to the dus family.
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CC   This Swiss-Prot entry is copyright. It is produced through a collaboration
CC   between  the Swiss Institute of Bioinformatics  and the  EMBL outstation -
CC   the European Bioinformatics Institute.  There are no  restrictions on  its
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DR   EMBL; Z37984; CAA86063.1; -.
DR   PIR; I39492; I39492.
DR   InterPro; IPR001269; Du_synth.
DR   InterPro; IPR004652; Du_synth_NifR3.
DR   InterPro; IPR003009; FMN_enzyme.
DR   Pfam; PF01207; Dus; 1.
DR   TIGRFAMs; TIGR00737; nifR3_yhdG; 1.
DR   PROSITE; PS01136; UPF0034; 1.
KW   FAD; Flavoprotein; Oxidoreductase; tRNA processing.
SQ   SEQUENCE   328 AA;  36098 MW;  700E5E1C61DBFFD1 CRC64;
     MAIQIGTISL EGPVILAPMS GVTDLPFRRL VKQSGCGLVV SEMVASQAMI RENRQTLRMV
     ECEPEQFPMA VQLAGCEPDV MAEAAKLNED RGAAIIDINF GCPVKKVVNG HAGSSLMRDE
     ALAARILEAT AKAVTIPVTL KMRKGWDDSS LNAPRLARIA EECGIKLVTV HGRTREQFYN
     GTADWSFIRS VKEAVSIPVV VNGDITSFDA VDRALAESGA DGVMIGRGAY GRPWFPAQVM
     HYIRTGERLP DPPLHEQLDT LLEHYDAMLT HYGVEGGLRV ARKHISWYST GLRDSAEFRS
     EVNRMSDPER VRGFIRDFYA PAIERMAA
//