ID   NIFA_AZOLI     STANDARD;      PRT;   624 AA.
AC   P54929;
DT   01-OCT-1996 (Rel. 34, Created)
DT   01-OCT-1996 (Rel. 34, Last sequence update)
DT   05-JUL-2004 (Rel. 44, Last annotation update)
DE   Nif-specific regulatory protein.
GN   Name=nifA;
OS   Azospirillum lipoferum.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales;
OC   Rhodospirillaceae; Azospirillum.
OX   NCBI_TaxID=193;
RN   [1]
RP   NUCLEOTIDE SEQUENCE.
RA   Shigematsu T., Hidaka M., Masaki H., Uozumi T.;
RL   Submitted (DEC-1992) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Required for activation of most nif operons, which are
CC       directly involved in nitrogen fixation.
CC   -!- SUBUNIT: Interacts with sigma-54.
CC   -!- SIMILARITY: Contains 1 sigma-54 factor interaction ATP-binding
CC       domain.
CC   --------------------------------------------------------------------------
CC   This Swiss-Prot entry is copyright. It is produced through a collaboration
CC   between  the Swiss Institute of Bioinformatics  and the  EMBL outstation -
CC   the European Bioinformatics Institute.  There are no  restrictions on  its
CC   use as long as its content is in no way modified and this statement is not
CC   removed.
CC   --------------------------------------------------------------------------
DR   EMBL; D13799; BAA02956.1; -.
DR   InterPro; IPR003593; AAA_ATPase.
DR   InterPro; IPR008931; FIS-like.
DR   InterPro; IPR003018; GAF.
DR   InterPro; IPR002197; HTH_Fis.
DR   InterPro; IPR010113; NifA.
DR   InterPro; IPR002078; Sig54_interact.
DR   Pfam; PF01590; GAF; 1.
DR   Pfam; PF02954; HTH_8; 1.
DR   Pfam; PF00158; Sigma54_activat; 1.
DR   PRINTS; PR01590; HTHFIS.
DR   SMART; SM00382; AAA; 1.
DR   SMART; SM00065; GAF; 1.
DR   TIGRFAMs; TIGR01199; HTH_fis; 1.
DR   TIGRFAMs; TIGR01817; nifA; 1.
DR   PROSITE; PS00675; SIGMA54_INTERACT_1; 1.
DR   PROSITE; PS00676; SIGMA54_INTERACT_2; 1.
DR   PROSITE; PS00688; SIGMA54_INTERACT_3; 1.
DR   PROSITE; PS50045; SIGMA54_INTERACT_4; 1.
KW   Activator; ATP-binding; DNA-binding; Metal-binding; Nitrogen fixation;
KW   Transcription regulation; Two-component regulatory system.
FT   DOMAIN      203    431       Sigma-54 factor interaction (Potential).
FT   DOMAIN      432    581       Inter-domain linker.
FT   DOMAIN      582    624       C-terminal DNA-binding domain.
FT   NP_BIND     231    238       ATP (Potential).
FT   NP_BIND     294    303       ATP (Potential).
FT   METAL       445    445       By similarity.
FT   METAL       450    450       By similarity.
FT   DNA_BIND    596    615       H-T-H motif (By similarity).
SQ   SEQUENCE   624 AA;  67119 MW;  223A36AD444E7016 CRC64;
     MPGAMRQSTS NLELLTIYEV SKILGSSLDL QQTLREVLRA LAYQLQMHRG RVYLVGEDNV
     LRLVAANGLS NEAAAQIEFR DGEGITGRIL KTGMPAVVPN LAEEPLFLNR TGGREDLDEQ
     VASLVGVPIK AAGVVVGVLT IDRISDEGPQ GHFGSDVRFL TMVANLIGQT VRLPHVAEEP
     LRDAETFRMQ KELRPIAAPI NDVVCTSPNM LEVMAQVHRV APFKSTVLIR GESGTGKELI
     ARAIHNMSPR KDAPFIRVNC AALPESLLES ELFGHEKGAF TGAQKDHKGR FELASGGTLF
     LDEIGDISPN FQAKLLRVLQ EQEFERVGGS KTIKTDVRLI CATNLNLEEA IGHGKFRADL
     YFRINVVTIH LPPLRERRQD IGPLARHFVA KFAKDNGMTL VMEDEALEVL NRCTWPGNVR
     ELENCIERAA TQSRDGIIRT ESLSCSLNLC NSSVLFQYRT LGASVGGLAP SMGPGSVNRV
     PPGRPGVPAP ANAPKAPAMP APVPEPAGAG SAWPACASGC SAGPSPVCGA AQPAVPVPLI
     PLPLPEPSAP AAAAPAPTSV TNAAPPPAAE VPLDEPESGS LRDRLLWAME RTGWVQAKAA
     RLLGMTTRQV SYALRKYNIE IKRF
//