ID MTA1_AZOBR STANDARD; PRT; 140 AA. AC P58284; DT 16-OCT-2001 (Rel. 40, Created) DT 16-OCT-2001 (Rel. 40, Last sequence update) DT 25-OCT-2004 (Rel. 45, Last annotation update) DE Modification methylase AbrI (EC 2.1.1.72) (Adenine-specific DE methyltransferase AbrI) (M.AbrI) (Fragment). GN Name=abrIM; OS Azospirillum brasilense. OC Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales; OC Rhodospirillaceae; Azospirillum. OX NCBI_TaxID=192; RN [1] RP NUCLEOTIDE SEQUENCE. RC STRAIN=ATCC 29711; RA Schwabe G., Helke A., Klingmueller W.; RT "AbrI restriction modification system from Azospirillium brasilense, RT molecular cloning and characterization of its genes."; RL Submitted (OCT-1991) to the EMBL/GenBank/DDBJ databases. CC -!- FUNCTION: This methylase recognizes the double-stranded sequence CC CTCGAG, causes specific methylation on A-5 on both strands, and CC protects the DNA from cleavage by the AbrI endonuclease CC (Potential). CC -!- CATALYTIC ACTIVITY: S-adenosyl-L-methionine + DNA adenine = S- CC adenosyl-L-homocysteine + DNA 6-methylaminopurine. CC -!- SIMILARITY: Belongs to the N4/N6-methyltransferase family. CC -------------------------------------------------------------------------- CC This Swiss-Prot entry is copyright. It is produced through a collaboration CC between the Swiss Institute of Bioinformatics and the EMBL outstation - CC the European Bioinformatics Institute. There are no restrictions on its CC use as long as its content is in no way modified and this statement is not CC removed. CC -------------------------------------------------------------------------- DR EMBL; X62690; -; NOT_ANNOTATED_CDS. DR REBASE; 4921; M.AbrI. KW Methyltransferase; Restriction system; Transferase. FT NON_TER 1 1 SQ SEQUENCE 140 AA; 15900 MW; 7B5BC7D96697E174 CRC64; YRTIDRIYPA LTREHKLLIP DIKGEAHIVY EEGRLYPHHN LYYITSEIWD LRALQAVLLS GIARLFVSVY STKMHGGFLR FQAQYLRRIR VPNWSQVPAA VRQELITAGA KPDLAACNRA VFALYAMTAE ERAALGGNGD //