ID GLTB_AZOBR STANDARD; PRT; 1515 AA. AC Q05755; DT 01-FEB-1995 (Rel. 31, Created) DT 01-FEB-1995 (Rel. 31, Last sequence update) DT 25-OCT-2004 (Rel. 45, Last annotation update) DE Glutamate synthase [NADPH] large chain precursor (EC 1.4.1.13) DE (Glutamate synthase alpha subunit) (NADPH-GOGAT) (GLTS alpha chain). GN Name=gltB; OS Azospirillum brasilense. OC Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales; OC Rhodospirillaceae; Azospirillum. OX NCBI_TaxID=192; RN [1] RP NUCLEOTIDE SEQUENCE, AND PROTEIN SEQUENCE OF 37-56; 778-799 AND RP 1325-1345. RC STRAIN=Sp7 / ATCC 29145; RX MEDLINE=93155143; PubMed=8428988; RA Pelanda R., Vanoni M.A., Perego M., Piubelli L., Galizzi A., Curti B., RA Zanetti G.; RT "Glutamate synthase genes of the diazotroph Azospirillum brasilense. RT Cloning, sequencing, and analysis of functional domains."; RL J. Biol. Chem. 268:3099-3106(1993). RN [2] RP NUCLEOTIDE SEQUENCE OF 834-927. RC STRAIN=RG; RX MEDLINE=94075244; PubMed=7902833; RA Mandal A.K., Ghosh S.; RT "Isolation of a glutamate synthase (GOGAT)-negative, pleiotropically N RT utilization-defective mutant of Azospirillum brasilense: cloning and RT partial characterization of GOGAT structural gene."; RL J. Bacteriol. 175:8024-8029(1993). RN [3] RP PARTIAL PROTEIN SEQUENCE. RX MEDLINE=90335272; PubMed=2198943; DOI=10.1016/0167-4838(90)90273-I; RA Vanoni M.A., Negri A., Zanetti G., Ronchi S., Curti B.; RT "Structural studies on the subunits of glutamate synthase from RT Azospirillum brasilense."; RL Biochim. Biophys. Acta 1039:374-377(1990). CC -!- CATALYTIC ACTIVITY: 2 L-glutamate + NADP(+) = L-glutamine + 2- CC oxoglutarate + NADPH. CC -!- COFACTOR: Binds a 3Fe-4S cluster; FAD and FMN. CC -!- PATHWAY: Nitrogen metabolism, glutamate biosynthesis. The CC catalyzed reaction brings together the nitrogen and carbon CC metabolism. CC -!- SUBUNIT: Aggregate of 4 catalytical active heterodimers, CC consisting of a large and a small subunit. CC -!- MISCELLANEOUS: Glutamine binds to the large subunit and transfers CC the amido group to 2-oxo-glutamate that apparently binds to the CC small subunit. CC -!- SIMILARITY: Belongs to the glutamate synthase family. CC -------------------------------------------------------------------------- CC This Swiss-Prot entry is copyright. It is produced through a collaboration CC between the Swiss Institute of Bioinformatics and the EMBL outstation - CC the European Bioinformatics Institute. There are no restrictions on its CC use as long as its content is in no way modified and this statement is not CC removed. CC -------------------------------------------------------------------------- DR EMBL; AF192408; AAA22179.1; -. DR EMBL; X71632; CAA50639.1; -. DR PIR; B46602; B46602. DR PDB; 1EA0; X-ray; A/B=37-1515. DR InterPro; IPR003009; FMN_enzyme. DR InterPro; IPR006982; Glu_synth_centr. DR InterPro; IPR006981; Glu_synth_NTN. DR InterPro; IPR002932; Glu_synthase. DR InterPro; IPR002489; Glu_synthase_C. DR Pfam; PF04898; Glu_syn_central; 1. DR Pfam; PF04897; Glu_synth_NTN; 1. DR Pfam; PF01645; Glu_synthase; 1. DR Pfam; PF01493; GXGXG; 1. KW 3D-structure; 3Fe-4S; Direct protein sequencing; FAD; Flavoprotein; KW FMN; Glutamate biosynthesis; Iron-sulfur; NADP; Oxidoreductase; KW Zymogen. FT PROPEP 1 36 FT CHAIN 37 1515 Glutamate synthase [NADPH] large chain. FT NP_BIND 1085 1142 FMN (By similarity). FT METAL 1138 1138 Iron-sulfur (3Fe-4S) (By similarity). FT METAL 1144 1144 Iron-sulfur (3Fe-4S) (By similarity). FT METAL 1149 1149 Iron-sulfur (3Fe-4S) (By similarity). FT NP_BIND 1086 1142 FMN (By similarity). FT STRAND 38 39 FT STRAND 41 44 FT TURN 45 46 FT STRAND 50 50 FT HELIX 51 61 FT TURN 62 62 FT HELIX 65 67 FT TURN 72 73 FT STRAND 80 80 FT STRAND 82 85 FT HELIX 88 97 FT TURN 98 99 FT STRAND 107 114 FT HELIX 119 135 FT TURN 136 137 FT STRAND 139 145 FT HELIX 150 152 FT HELIX 155 160 FT STRAND 163 170 FT TURN 172 173 FT HELIX 177 198 FT TURN 199 199 FT STRAND 204 209 FT STRAND 212 214 FT STRAND 217 217 FT HELIX 221 223 FT HELIX 224 227 FT HELIX 229 232 FT TURN 234 235 FT STRAND 238 238 FT STRAND 240 245 FT TURN 256 257 FT STRAND 264 268 FT TURN 271 272 FT HELIX 273 283 FT HELIX 284 286 FT TURN 291 292 FT HELIX 293 295 FT HELIX 296 299 FT TURN 305 306 FT HELIX 309 322 FT TURN 323 324 FT HELIX 327 334 FT HELIX 347 359 FT STRAND 367 371 FT STRAND 376 380 FT TURN 383 384 FT STRAND 390 394 FT TURN 395 396 FT STRAND 398 401 FT TURN 406 407 FT HELIX 412 414 FT STRAND 415 420 FT TURN 423 424 FT STRAND 426 430 FT TURN 431 434 FT STRAND 435 437 FT HELIX 439 448 FT TURN 449 451 FT HELIX 452 456 FT TURN 457 458 FT STRAND 460 460 FT TURN 462 463 FT HELIX 464 471 FT TURN 472 473 FT TURN 480 480 FT HELIX 481 488 FT TURN 489 491 FT HELIX 494 498 FT TURN 499 501 FT HELIX 502 507 FT STRAND 512 513 FT HELIX 522 524 FT HELIX 531 533 FT TURN 534 534 FT STRAND 535 537 FT TURN 549 552 FT HELIX 553 555 FT STRAND 560 562 FT TURN 568 569 FT HELIX 573 575 FT STRAND 579 581 FT STRAND 585 585 FT HELIX 588 598 FT HELIX 599 601 FT STRAND 602 606 FT STRAND 608 610 FT TURN 612 613 FT TURN 615 616 FT HELIX 617 635 FT TURN 636 636 FT STRAND 639 643 FT TURN 645 646 FT STRAND 648 648 FT TURN 649 650 FT STRAND 651 653 FT HELIX 656 668 FT TURN 669 671 FT HELIX 673 675 FT STRAND 677 681 FT HELIX 688 695 FT TURN 696 698 FT STRAND 701 703 FT HELIX 705 715 FT TURN 716 722 FT HELIX 725 745 FT TURN 746 748 FT HELIX 752 755 FT TURN 756 757 FT STRAND 761 763 FT STRAND 766 766 FT HELIX 768 774 FT STRAND 785 785 FT HELIX 787 802 FT HELIX 828 840 FT TURN 841 841 FT HELIX 843 854 FT TURN 855 855 FT HELIX 861 864 FT STRAND 865 867 FT HELIX 876 878 FT HELIX 882 886 FT TURN 887 888 FT STRAND 889 893 FT STRAND 896 896 FT TURN 897 898 FT STRAND 899 899 FT HELIX 901 913 FT TURN 914 915 FT STRAND 917 919 FT TURN 922 923 FT HELIX 927 929 FT STRAND 932 932 FT TURN 934 935 FT STRAND 938 938 FT STRAND 943 946 FT TURN 951 952 FT HELIX 955 958 FT TURN 959 959 FT STRAND 963 967 FT TURN 971 972 FT TURN 974 976 FT STRAND 979 980 FT HELIX 982 984 FT HELIX 987 993 FT TURN 994 994 FT TURN 997 998 FT STRAND 1001 1002 FT TURN 1008 1009 FT HELIX 1013 1026 FT TURN 1028 1029 FT STRAND 1031 1037 FT TURN 1040 1041 FT HELIX 1042 1051 FT TURN 1052 1053 FT STRAND 1056 1060 FT TURN 1062 1063 FT STRAND 1069 1070 FT TURN 1071 1072 FT HELIX 1073 1076 FT HELIX 1081 1093 FT TURN 1094 1096 FT TURN 1098 1100 FT STRAND 1102 1106 FT HELIX 1112 1120 FT TURN 1121 1122 FT STRAND 1125 1127 FT HELIX 1130 1136 FT TURN 1137 1137 FT TURN 1144 1147 FT TURN 1150 1151 FT TURN 1158 1159 FT HELIX 1160 1162 FT HELIX 1168 1189 FT TURN 1190 1190 FT HELIX 1195 1197 FT TURN 1198 1199 FT HELIX 1201 1203 FT STRAND 1204 1206 FT HELIX 1220 1223 FT TURN 1247 1247 FT HELIX 1248 1254 FT TURN 1255 1255 FT HELIX 1256 1261 FT STRAND 1265 1271 FT TURN 1273 1274 FT TURN 1278 1279 FT HELIX 1280 1289 FT TURN 1290 1293 FT TURN 1297 1298 FT STRAND 1300 1308 FT TURN 1312 1315 FT STRAND 1317 1317 FT TURN 1318 1319 FT STRAND 1320 1328 FT TURN 1332 1335 FT STRAND 1337 1344 FT TURN 1347 1348 FT HELIX 1353 1355 FT STRAND 1357 1359 FT TURN 1363 1366 FT STRAND 1369 1374 FT STRAND 1377 1378 FT TURN 1380 1385 FT STRAND 1387 1393 FT STRAND 1396 1396 FT TURN 1400 1403 FT STRAND 1406 1409 FT STRAND 1415 1415 FT STRAND 1419 1419 FT TURN 1420 1422 FT STRAND 1427 1428 FT TURN 1433 1434 FT HELIX 1437 1440 FT STRAND 1441 1441 FT HELIX 1443 1445 FT STRAND 1446 1449 FT TURN 1454 1455 FT HELIX 1456 1471 FT HELIX 1474 1481 FT TURN 1482 1482 FT HELIX 1483 1487 FT TURN 1488 1489 FT STRAND 1492 1495 FT HELIX 1496 1501 SQ SEQUENCE 1515 AA; 166018 MW; ED913218DBFCEE92 CRC64; MTTELNQGEQ FVADFRANAA ALTTANAYNP EDEHDACGVG FIAAIDGKPR RSVVEKGIEA LKAVWHRGAV DADGKTGDGA GIHVAVPQKF FKDHVKVIGH RAPDNKLAVG QVFLPRISLD AQEACRCIVE TEILAFGYYI YGWRQVPINV DIIGEKANAT RPEIEQIIVG NNKGVSDEQF ELDLYIIRRR IEKAVKGEQI NDFYICSLSA RSIIYKGMFL AEQLTTFYPD LLDERFESDF AIYHQRYSTN TFPTWPLAQP FRMLAHNGEI NTVKGNVNWM KAHETRMEHP AFGTHMQDLK PVIGVGLSDS GSLDTVFEVM VRAGRTAPMV KMMLVPQALT SSQTTPDNHK ALIQYCNSVM EPWDGPAALA MTDGRWVVGG MDRNGLRPMR YTITTDGLII GGSETGMVKI DETQVIEKGR LGPGEMIAVD LQSGKLYRDR ELKDHLATLK PWDKWVQNTT HLDELVKTAS LKGEPSDMDK AELRRRQQAF GLTMEDMELI LHPMVEDGKE AIGSMGDDSP IAVLSDKYRG LHHFFRQNFS QVTNPPIDSL RERRVMSLKT RLGNLGNILD EDETQTRLLQ LESPVLTTAE FRAMRDYMGD TAAEIDATFP VDGGPEALRD ALRRIRQETE DAVRGGATHV ILTDEAMGPA RAAIPAILAT GAVHTHLIRS NLRTFTSLNV RTAEGLDTHY FAVLIGVGAT TVNAYLAQEA IAERHRRGLF GSMPLEKGMA NYKKAIDDGL LKIMSKMGIS VISSYRGGGN FEAIGLSRAL VAEHFPAMVS RISGIGLNGI QKKVLEQHAT AYNEEVVALP VGGFYRFRKS GDRHGWEGGV IHTLQQAVTN DSYTTFKKYS EQVNKRPPMQ LRDLLELRST KAPVPVDEVE SITAIRKRFI TPGMSMGALS PEAHGTLNVA MNRIGAKSDS GEGGEDPARF RPDKNGDNWN SAIKQVASGR FGVTAEYLNQ CRELEIKVAQ GAKPGEGGQL PGFKVTEMIA RLRHSTPGVM LISPPPHHDI YSIEDLAQLI YDLKQINPDA KVTVKLVSRS GIGTIAAGVA KANADIILIS GNSGGTGASP QTSIKFAGLP WEMGLSEVHQ VLTLNRLRHR VRLRTDGGLK TGRDIVIAAM LGAEEFGIGT ASLIAMGCIM VRQCHSNTCP VGVCVQDDKL RQKFVGTPEK VVNLFTFLAE EVREILAGLG FRSLNEVIGR TDLLHQVSRG AEHLDDLDLN PRLAQVDPGE NARYCTLQGR NEVPDTLDAR IVADARPLFE EGEKMQLAYN ARNTQRAIGT RLSSMVTRKF GMFGLQPGHI TIRLRGTAGQ SLGAFAVQGI KLEVMGDAND YVGKGLSGGT IVVRPTTSSP LETNKNTIIG NTVLYGATAG KLFAAGQAGE RFAVRNSGAT VVVEGCGSNG CEYMTGGTAV ILGRVGDNFA AGMTGGMAYV YDLDDSLPLY INDESVIFQR IEVGHYESQL KHLIEEHVTE TQSRFAAEIL NDWAREVTKF WQVVPKEMLN RLEVPVHLPK AISAE //