ID   AF037600   standard; genomic DNA; PRO; 1381 BP.
XX
AC   AF037600;
XX
SV   AF037600.1
XX
DT   21-AUG-1998 (Rel. 56, Created)
DT   21-AUG-1998 (Rel. 56, Last updated, Version 1)
XX
DE   Azotobacter vinelandii alginate lyase (algL) gene, complete cds.
XX
KW   .
XX
OS   Azotobacter vinelandii
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Azotobacter.
XX
RN   [1]
RP   1-1381
RX   MEDLINE; 96427318.
RX   PUBMED; 8830682.
RA   Rehm B.H., Ertesvag H., Valla S.;
RT   "A new Azotobacter vinelandii mannuronan C-5-epimerase gene (algG) is part
RT   of an alg gene cluster physically organized in a manner similar to that in
RT   Pseudomonas aeruginosa";
RL   J. Bacteriol. 178(20):5884-5889(1996).
XX
RN   [2]
RP   1-1381
RX   MEDLINE; 98348436.
RX   PUBMED; 9683471.
RA   Ertesvag H., Erlien F., Skjak-Braek G., Rehm B.H.A., Valla S.;
RT   "Biochemical properties and substrate specificities of a recombinantly
RT   produced Azotobacter vinelandii alginate lyase";
RL   J. Bacteriol. 180(15):3779-3784(1998).
XX
RN   [3]
RP   1-1381
RA   Ertesvaag H., Erlien F., Skjaak-Braek G., Rehm B.H.A., Valla S.;
RT   ;
RL   Submitted (10-DEC-1997) to the EMBL/GenBank/DDBJ databases.
RL   Unigen Center for Molecular Biology, Norwegian University of Technology and
RL   Science, Medisinsk Teknisk Senter, Trondheim N-7005, Norway
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1381
FT                   /db_xref="taxon:354"
FT                   /mol_type="genomic DNA"
FT                   /organism="Azotobacter vinelandii"
FT                   /strain="strain E"
FT   CDS             124..1248
FT                   /codon_start=1
FT                   /db_xref="GOA:O52195"
FT                   /db_xref="UniProt/Swiss-Prot:O52195"
FT                   /note="Active against acetylated substrate"
FT                   /transl_table=11
FT                   /gene="algL"
FT                   /function="splits ManA-ManA and ManA-GulA bonds"
FT                   /product="alginate lyase"
FT                   /protein_id="AAC32313.1"
FT                   /translation="MHKTRLALSCLLGSLLLSGAVHAAEALVPPKGYYAPVDIRKGEAP
FT                   ACPVVPEPFTGELVFRSKYEGSDAARSTLNEEAEKAFRTKTAPITQIERGVSRMVMRYM
FT                   EKGRAGDLECTLAWLDAWAEDGALLTTEYNHTGKSMRKWALGSLAGAYLRLKFSSSQPL
FT                   AAYPEQARRIESWFAKVGDQVIKDWSDLPLKRINNHSYWAAWAVMAAGVATNRRPLFDW
FT                   AVEQFHIAAGQVDSNGFLPNELKRRQRALAYHNYSLPPLMMVAAFALANGVDLRGDNDG
FT                   ALGRLAGNVLAGVEKPEPFAERAGDEDQDMEDLETDAKFSWLEPYCALYSCSPALRERK
FT                   AEMGPFKNFRLGGDVTRIFDPAEKSPRSTVGKRD"
XX
SQ   Sequence 1381 BP; 251 A; 504 C; 440 G; 186 T; 0 other;
     ccatggaaat ccagccgccg gagggctcaa ggagcccgtc gaggtcgagg tgcgcctctg        60
     caagcgccac gactatcacg ctccggccaa cctgaccgcc cgtagcggca actgaggcct       120
     accatgcaca agaccagact cgccctctcc tgcctgctcg gcagcctgct gttgagcggc       180
     gccgtccacg ccgccgaagc cctggttccg cccaagggct actacgcgcc ggtggatatc       240
     cgcaaaggcg aggcgccggc ctgtccggtg gtgcccgaac ccttcaccgg ggaactggtc       300
     tttcgcagca agtacgaagg ctcggacgcg gcccggtcga ccctcaacga ggaagcggaa       360
     aaggccttcc gcaccaagac cgcgccgatc acccagatcg agcgcggcgt cagccggatg       420
     gtcatgcgct acatggagaa aggccgcgcc ggcgacctgg aatgcaccct ggcctggctg       480
     gacgcctggg ccgaagacgg cgccctgctc accaccgagt acaaccacac cggcaaatcc       540
     atgcgcaagt gggccctcgg cagcctggcc ggcgcctacc tgcgcctgaa attctccagc       600
     tcgcagccgc tggcggccta tcccgaacag gcacggcgca tcgagagctg gttcgccaag       660
     gtgggcgacc aggtgatcaa ggactggagc gacctgccgc tcaagcggat caacaaccac       720
     tcctactggg cggcctgggc ggtcatggcc gccggcgtgg cgaccaaccg ccggccgctg       780
     ttcgactggg cggtcgagca gttccacatc gccgccgggc aggtcgactc gaatggcttc       840
     ctgcccaacg aactcaagcg ccgccaacgt gcgctggcct accacaacta cagcttgccg       900
     cccctgatga tggtcgccgc cttcgccctg gccaacggtg tcgacctgcg cggcgacaac       960
     gatggcgccc tgggccgcct ggccggcaac gtgctggccg gcgtcgagaa gcccgaaccg      1020
     ttcgccgaac gggccggcga cgaggatcag gacatggagg atctggagac cgacgccaag      1080
     ttctcctggc tggaacccta ctgcgcgctc tactcctgct cccccgccct gcgggaaagg      1140
     aaggccgaga tgggcccgtt caagaatttc cgcctgggtg gcgacgtgac ccggatcttc      1200
     gatccggccg agaagtcgcc cagatcgacc gtcggcaagc gcgactagcg cggccccgac      1260
     gtcgccccag cagccggcag tcagtccgtc gccggccgcc acgcccttcc gggccaggcg      1320
     gaatgccccg aaatgcgcaa cagacgcagc cgggaacgga cctcttcggg gaccccccgg      1380
     g                                                                      1381
//