ID AF099799 standard; genomic DNA; PRO; 2965 BP. XX AC AF099799; XX SV AF099799.1 XX DT 16-NOV-1998 (Rel. 57, Created) DT 18-JAN-1999 (Rel. 58, Last updated, Version 2) XX DE Azotobacter vinelandii mannuronan C-5-epimerase AlgE6 (algE6) gene, DE complete cds. XX KW . XX OS Azotobacter vinelandii OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; OC Pseudomonadaceae; Azotobacter. XX RN [1] RP 1-2965 RX MEDLINE; 99084941. RX PUBMED; 9864314. RA Svanem B.I.G., Skjaak-Braek G., Ertesvaag H., Valla S.; RT "Cloning and expression of three new Azotobacter vinelandii genes closely RT related to a previously described gene family encoding mannuronan RT C-5-epimerases"; RL J. Bacteriol. 181(1):68-77(1999). XX RN [2] RP 1-2965 RA Svanem B.I.G., Skjaak-Braek G., Ertesvaag H., Valla S.; RT ; RL Submitted (20-OCT-1998) to the EMBL/GenBank/DDBJ databases. RL Unigen, MTS, Norwegian University of Science and Technology, Olav Kyrres RL gt.3, Trondheim 7030, Norway XX FH Key Location/Qualifiers FH FT source 1..2965 FT /db_xref="taxon:354" FT /mol_type="genomic DNA" FT /organism="Azotobacter vinelandii" FT /strain="E" FT CDS 238..2862 FT /codon_start=1 FT /db_xref="GOA:Q9ZFH0" FT /db_xref="UniProt/Swiss-Prot:Q9ZFH0" FT /transl_table=11 FT /gene="algE6" FT /function="introduces blocks of guluronic acid residues FT into its substrate" FT /function="catalyzes Ca+2-dependent polymer-level FT epimerization of mannuronic acid to guluronic acid in the FT commercially important polysaccharide alginate" FT /product="mannuronan C-5-epimerase AlgE6" FT /protein_id="AAD04920.1" FT /translation="MDYNVKDFGALGDGVSDDRVAIQAAIDAAHAAGGGTVYLPPGEYR FT VSAAGEPSDGCLTLRDNVYLAGAGMGQTVIKLVDGSAQKITGIVRSPFGEETSNFGMRD FT LTLDGNRANTVDKVDGWFNGYAPGQPGADRNVTIERVEVREMSGYGFDPHEQTINLVLR FT DSVAHHNGLDGFVADYQIGGTFENNVAYANDRHGFNIVTSTNDFVMRNNVAYGNGGNGL FT VVQRGSENLAHPENILIDGGSYYDNGLEGVLVKMSNNVTVQNADIHGNGSSGVRVYGAQ FT GVQILGNQIHDNAKTAVAPEVLLQSYDDTLGVSGNYYTTLNTRVEGNTITGSANSTYGV FT QERNDGTDFSSLVGNTINGVQEAAHLYGPNSTVSGTVSAPPQGTDGNDVLIGSDVGEQI FT SGGAGDDRLDGGAGDDLLDGGAGRDRLTGGLGADTFRFALREDSHRSPLGTFSDLILDF FT DPSQDKIDVSALGFIGLGNGYAGTLAVSLSADGLRTYLKSYDADAQGRSFELALDGNHA FT ATLSAGNIVFAAATPVDPSAEAQPIVGSDLDDQLHGTLLGEEISGGGGADQLYGYGGGD FT LLDGGAGRDRLTGGEGADTFRFALREDSHRSAAGTFSDLILDFDPTQDKLDVSALGFTG FT LGNGYAGTLAVSVSDDGTRTYLKSYETDAEGRSFEVSLQGNHAAALSADNILFATPVPV FT DPGVEGTPVVGSDLDDELHGTLGSEQILGGGGADQLYGYAGNDLLDGGAGRDKLSGGEG FT ADTFRFALREDSHRSPLGTFGDRILDFDPSQDRIDVSALGFSGLGNGYAGSLAVSVSDD FT GTRTYLKSYEADAQGLSFEVALEGDHAAALSADNIVFAATDAAAAGELGVIGASGQPDD FT PAV" FT misc_feature 238..1389 FT /note="encodes A-module which in active epimerase contains FT alginate binding site" FT /gene="algE6" FT misc_feature 1390..1830 FT /note="encodes R1-module which in active epimerase binds FT Ca+2" FT /gene="algE6" FT misc_feature 1861..2313 FT /note="encodes R2-module which in active epimerase binds FT Ca+2" FT /gene="algE6" FT misc_feature 2344..2796 FT /note="encodes R3-module which in active epimerase binds FT Ca+2" FT /gene="algE6" XX SQ Sequence 2965 BP; 516 A; 1070 C; 983 G; 396 T; 0 other; acggcgtttc ccgcgagcgg cggaccgccg ttcgcgggac gaatcctttc cacccctcat 60 ccacggcaac gcaaggaatg ctttgcccgg gcgtcgtacg cggggcttcg ccaagggccg 120 ccgcgggcgt caggcgcaag gaccgggcaa tcgcgtcaga ggctggagcg gacgttctcg 180 cccgctcctc tttccaagcg gcttcggctg cctcacatgg gatcgggaag cggagatatg 240 gattacaacg tcaaggattt cggagcactg ggcgacggcg tcagcgacga ccgggtcgcc 300 atccaggcgg cgatcgacgc cgctcacgcc gcgggcggcg gtaccgtcta cctgccgccc 360 ggcgaatacc gggtcagcgc cgccggggaa ccttccgacg gctgcctgac gctcagggac 420 aacgtctacc tggccggcgc cggcatgggc cagacggtga tcaaactggt cgacggctcg 480 gcccagaaga tcaccggcat agtccgctcg cccttcggcg aggaaaccag caacttcggc 540 atgcgcgacc tgaccctgga cggcaaccgc gccaacaccg tcgacaaggt cgacggctgg 600 ttcaacggct atgcccccgg ccagccgggc gccgatcgca acgtgaccat cgagcgggtg 660 gaggtccgcg agatgtccgg ctacggcttc gacccccacg agcagaccat caacctggtg 720 ctgcgcgaca gcgtggccca tcacaacggc ctcgacggct tcgtcgccga ctaccagatc 780 ggcggaacct tcgagaacaa cgtcgcctac gccaacgacc gccacggctt caacatcgtc 840 accagcacca acgacttcgt catgcgcaac aacgtcgcct acggcaacgg cggcaacggc 900 ctggtggtgc agcggggttc ggaaaacctc gcccatcccg agaatatcct gatcgacggc 960 ggctcctact acgacaacgg cctggaaggc gtgctggtca agatgagcaa caacgtcacc 1020 gtgcagaacg ccgatatcca cggcaacggc tcctccgggg tgcgcgtcta cggcgcccag 1080 ggcgtgcaga tcctcggcaa ccagatccac gacaacgcga agacggccgt cgccccggaa 1140 gtgctgctgc agtcctacga cgataccctc ggcgtgtccg gcaactacta cacgaccctg 1200 aacacccggg tcgagggcaa caccatcacc ggctcggcca actccaccta cggcgtgcag 1260 gagcgcaacg acggcaccga cttcagcagc ctggtcggca acaccatcaa cggcgtgcag 1320 gaggctgccc acctgtacgg tccgaattcg acggtctccg gcactgtcag cgcgccgccg 1380 caagggaccg acggcaacga cgtgctgatc ggcagcgacg tcggcgagca gatcagcggc 1440 ggagccggcg acgatcgcct ggacggcggg gccggcgacg acctgctcga cggcggcgcc 1500 gggcgcgacc ggctgaccgg cggcctgggc gccgacacct tccgcttcgc cctgcgcgag 1560 gacagccatc gctcgccgct gggcaccttc agcgacctga tcctcgactt cgatccgagc 1620 caggacaaga tcgacgtgtc ggcgctcggt ttcatcggcc tgggcaacgg ctacgccggc 1680 accctggcgg tgagcctcag cgccgacggc ctgcgcacct acctgaagag ctacgacgcg 1740 gacgcccagg gccgctcctt cgaactggcg ctggacggca accatgccgc gaccctgtcg 1800 gcgggcaaca tcgtcttcgc cgcggccacc ccggtcgacc ccagcgccga ggcgcaaccg 1860 atcgtcggca gcgacctcga cgaccagttg cacggcaccc tgctcggcga ggagatcagc 1920 ggcggcggcg gcgccgacca attgtacggc tatggcggcg gcgacctgct cgacggcggc 1980 gccgggcgcg accggctgac cggcggcgag ggcgccgaca ccttccgctt cgccctgcgc 2040 gaggacagcc accgctcggc ggcgggcacc ttcagcgacc tgatcctcga cttcgatccg 2100 acccaggaca agctcgacgt atcggcgctt ggcttcaccg gcctgggcaa cggctacgcc 2160 ggcaccctgg cggtgagcgt cagcgacgac ggcacgcgca cctacctgaa gagctacgag 2220 acggatgccg agggccgctc cttcgaggtc agcctgcagg gcaaccatgc cgccgcgctg 2280 tcggccgaca acatcctgtt cgccacgccc gtgccggtcg atcccggcgt cgagggaacg 2340 ccggtggtcg gcagcgacct cgacgacgag ttgcacggca ccctgggcag cgagcagatc 2400 ctcggcggcg gcggcgccga ccagttgtac ggctacgccg gcaacgacct gctcgacggc 2460 ggcgccggtc gcgacaagct gagcggcggc gagggcgccg acaccttccg cttcgccctg 2520 cgcgaggaca gccaccgctc gccgttgggg accttcggcg accgcatcct cgacttcgat 2580 ccgagccagg acaggatcga cgtctcggcg ctgggctttt ccggcctggg caacggctat 2640 gccggtagcc tggcggtgag cgtcagcgac gacggcacgc gcacctacct gaagagctac 2700 gaggcggacg cccagggtct gagcttcgag gtggccctgg agggcgacca tgccgccgcg 2760 ctgtcggccg acaacatcgt cttcgccgcg accgacgctg cggcggccgg cgaactcggg 2820 gtgataggcg ccagcggcca gccggacgat ccggccgtct gagacggcgc ctccggccgt 2880 cggcgagtgc gccgttcgcc gacggccggg cgaacggatg aggactgctc cactctcacc 2940 cagataagcg cgtgggccgt ttcat 2965 //