ID AF099800 standard; genomic DNA; PRO; 2992 BP. XX AC AF099800; XX SV AF099800.1 XX DT 16-NOV-1998 (Rel. 57, Created) DT 18-JAN-1999 (Rel. 58, Last updated, Version 2) XX DE Azotobacter vinelandii mannuronan C-5-epimerase AlgE7 (algE7) gene, DE complete cds. XX KW . XX OS Azotobacter vinelandii OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; OC Pseudomonadaceae; Azotobacter. XX RN [1] RP 1-2992 RX MEDLINE; 99084941. RX PUBMED; 9864314. RA Svanem B.I.G., Skjaak-Braek G., Ertesvaag H., Valla S.; RT "Cloning and expression of three new Azotobacter vinelandii genes closely RT related to a previously described gene family encoding mannuronan RT C-5-epimerases"; RL J. Bacteriol. 181(1):68-77(1999). XX RN [2] RP 1-2992 RA Svanem B.I.G., Skjaak-Braek G., Ertesvaag H., Valla S.; RT ; RL Submitted (20-OCT-1998) to the EMBL/GenBank/DDBJ databases. RL Unigen, MTS, Norwegian University of Science and Technology, Olav Kyrres RL gt.3, Trondheim 7030, Norway XX FH Key Location/Qualifiers FH FT source 1..2992 FT /db_xref="taxon:354" FT /mol_type="genomic DNA" FT /organism="Azotobacter vinelandii" FT /strain="E" FT CDS 237..2807 FT /codon_start=1 FT /db_xref="GOA:Q9ZFG9" FT /db_xref="UniProt/Swiss-Prot:Q9ZFG9" FT /transl_table=11 FT /gene="algE7" FT /function="introduces both single residue and short blocks FT of guluronic acid residues into its substrate" FT /function="catalyzes Ca+2-dependent polymer-level FT epimerization of mannuronic acid to guluronic acid in the FT commercially important polysaccharide alginate" FT /product="mannuronan C-5-epimerase AlgE7" FT /protein_id="AAD04921.1" FT /translation="MEYNVKDFGAKGDGKTDDTDAIQAAIDAAHKAGGGTVYLPSGEYR FT VSGGDEASDGALIIKSNVYIVGAGMGETVIKLVDGWDEKLTGIIRSANGEKTHDYGISD FT LTIDGNQDNTEGEVDGFYTGYIPGKNGADYNVTVERVEIREVSRYAFDPHEQTINLTIR FT DSVAHDNGKDGFVADFQIGAVFENNVSYNNGRHGFNIVTSSHDIVFTNNVAYGNGANGL FT VVQRGSEDRDFVYNVEIEGGSFHDNGQEGVLIKMSTDVTLQGAEIYGNGYAGVRVQGVE FT DVRILDNYIHDNAQSKANAEVIVESYDDRDGPSDDYYETQNVTVKGNTIVGSANSTYGI FT QERADGTDYTSIGNNSVSGTQRGIVQLSGTNSTFSGRSGDAYQFIDGSTGNDLLTGTPI FT ADLIVGGSGNDTLSGDAGNDVLEGGAGSDRLTGGEGADIFRFTAVSDSYYTASSSVADQ FT ILDFDASNDRIDLTGLGFTGLGDGYGGTLAVLANSDGSRTYLRSYEKDADGRYFSLTLD FT GNFVGRLDDSNLVFRHKTIAGTEGDDSLTGNAMAEILDGGSGNDSLAGGLGNDVLRGGA FT GDDILNGGLGRDQLSGGEGADIFRFTSVADSYQNSGDNFSDLILDFDPGEDRIDLSGLG FT FSGLGDGHNGTLLLWTSSETNRTYLKNFDTDADGRRFEIALEGVFSDLSEKQLVFERLV FT LEGTRLGDQLSGTELNEELLGGAGRDILNGGAGDDILDGGSERDTLTGGSGADVFRFNA FT TLDSFRNYDNGTSRVDDITDFTVGEDLIDLSALGYSGLGNGYDGTLAVLLNADGTKTYL FT KDRESDADGNHFEIALDGNYADQLSNGDFIFTNLEVIGSSSQAA" FT misc_feature 237..1388 FT /note="encodes A-module which in active epimerase contains FT alginate binding site" FT /gene="algE7" FT misc_feature 1389..1841 FT /note="encodes R1-module which in active epimerase binds FT Ca+2" FT /gene="algE7" FT misc_feature 1842..2318 FT /note="encodes R2-module which in active epimerase binds FT Ca+2" FT /gene="algE7" FT misc_feature 2319..2777 FT /note="encodes R3-module which in active epimerase binds FT Ca+2" FT /gene="algE7" XX SQ Sequence 2992 BP; 620 A; 986 C; 922 G; 464 T; 0 other; ttcatgattc cctggagaac taataaaggc ttccatagtc ggaaaactca cggcaacgcg 60 tcggccagta tttccggaac tcttttcttg cccactcggg aaggggccgg tggtaggaag 120 ccgagtagtc gcgacgatgc cggaggcagg gatcgctgtt cgggcagccg gctttctccg 180 cctcgatttc cttcaagagc gttcggctgc gcagtttgca tacggaagcg aagcagatgg 240 aatacaacgt taaggatttt ggtgccaagg gtgatggcaa gacggacgac acggatgcca 300 tacaggcggc gatagatgcc gcccacaagg cggggggcgg gacggtatac ctgccgtccg 360 gcgaatatcg ggtcagcggt ggcgacgagg cctccgacgg cgctctgatc atcaagagca 420 acgtctatat cgtcggtgcc ggcatgggcg agacggtgat caagctggtc gatgggtggg 480 acgaaaagct caccggcatc atccgctcgg ccaacggcga gaaaacccat gattacggta 540 tcagcgacct gaccatcgac ggtaaccagg acaacaccga aggcgaggtc gacggcttct 600 ataccggcta tattcccggc aagaatggcg cggactacaa cgtcacggtc gaacgggtgg 660 agatccgcga ggtatcccgc tacgccttcg atccccacga gcagaccatc aacctgacga 720 tccgcgacag cgtcgcccac gacaacggca aggacgggtt cgtcgccgac ttccagatcg 780 gcgccgtgtt cgagaacaac gtctcgtaca acaacggccg ccacggcttc aacatcgtca 840 ccagcagtca cgacatcgtc ttcaccaaca acgtcgccta cggcaacggc gccaacggcc 900 tggtggtcca gcgcggctcg gaagaccggg acttcgtcta caacgtggag atcgagggcg 960 gctccttcca tgacaacggt caggaaggcg tgctgatcaa gatgagcacc gatgtcaccc 1020 tgcagggcgc cgagatctac ggcaacggct acgcgggcgt gcgcgtgcag ggcgtcgagg 1080 acgtgcggat cctcgacaac tacatccacg acaacgcaca gagcaaggcc aacgcggaag 1140 tcatcgtgga atcctacgac gaccgcgacg gcccgtccga cgactactac gaaacgcaga 1200 acgtcacggt caagggcaat accatcgtcg gttcggccaa ttccacctac ggcatccagg 1260 agcgcgccga cggcaccgac tacaccagca tcggcaacaa cagcgtcagc ggcacccagc 1320 gcgggatcgt gcagctctcg gggacgaact cgacgttctc cggcaggtcg ggcgatgcct 1380 accagttcat cgacggcagc accggcaatg acctgctgac cggtaccccg atcgccgatc 1440 tgatcgtggg cggcagcggc aacgacaccc tgagcggcga cgccggcaac gacgttctcg 1500 aaggcggtgc cggcagcgat cgcctgaccg gcggcgaggg cgccgacatc ttccgcttca 1560 cggcggtcag cgacagctat tacaccgcca gcagcagcgt cgccgaccag atcctcgact 1620 tcgacgccag caatgatcgc atcgacctca ccgggctcgg cttcaccggc ctgggcgacg 1680 gctacggcgg caccctggcc gtgctggcca acagcgacgg cagccgcacc tatctgcgca 1740 gctacgagaa ggacgccgac ggccgctatt tctcgctcac cctggacggc aacttcgtcg 1800 gtcggctcga cgacagcaac ctggtcttca ggcacaagac catcgccggc accgagggcg 1860 acgacagcct gaccggcaac gcgatggcgg aaatcctcga cggcggcagc ggcaacgaca 1920 gcctcgcggg cggtctgggc aacgacgtgc tgagaggcgg tgccggcgac gacatcctga 1980 acggcggcct ggggcgcgac cagctcagcg gcggcgaagg cgcggacata ttccgcttca 2040 ccagcgtggc cgacagctac cagaactcgg gcgacaactt ctccgacctg attctcgatt 2100 tcgacccggg cgaagaccgc atcgatctca gcggcctggg cttcagcggc ctgggcgacg 2160 gccacaacgg taccctgctg ctctggacca gcagcgaaac caaccgcacc tatctcaaga 2220 acttcgacac ggatgccgac ggccggcgct tcgagatcgc cctggagggc gtcttctccg 2280 acctgagcga gaagcaactg gtcttcgaac gcctggtact ggagggcact cgcctcggcg 2340 accagctttc cggcaccgag ctgaacgagg aactgctcgg cggcgcgggg cgcgacatcc 2400 tgaacggcgg cgccggcgac gatattctcg atggcggttc cgaacgcgac accctgaccg 2460 gcggcagcgg cgcggacgtg ttccgcttca acgccacgct ggacagcttc cgcaactacg 2520 acaatgggac gagccgggtc gacgacatca ccgacttcac cgtcggcgag gatctgatcg 2580 acctctccgc cctcggctat agcggcctgg gcaacggcta cgacggcacg ctcgccgtgc 2640 tgctgaatgc cgacggcacc aagacctacc tcaaggaccg cgaaagcgat gcggacggca 2700 accacttcga gatcgccctg gacggcaact atgccgatca gctctccaac ggcgacttca 2760 tcttcaccaa cctcgaagtg atcggcagca gctcgcaggc tgcctgatgc ggctcggcct 2820 ggcgcggtcc ggcccggcct gatatgtcct ggcctggtgt gtccgggctc gacacgcccc 2880 cagtcgtcgg caagcctgcc cgattgccga cgatcgggcg aaccgatctg tttcttcccc 2940 agtctcaccc cttgaaacgt atggatcgtt tcattcgggc gctcttcgga gc 2992 //