ID   BM076967   standard; mRNA; EST; 289 BP.
XX
AC   BM076967;
XX
SV   BM076967.2
XX
DT   07-FEB-2002 (Rel. 70, Created)
DT   18-NOV-2003 (Rel. 77, Last updated, Version 2)
XX
DE   TrEST-A3651 TrEST-A Hypocrea jecorina cDNA clone Tr-A3651 5' similar to
DE   COG3199: Uncharacterized conserved protein [Azotobacter vinelandii], mRNA
DE   sequence.
XX
KW   EST.
XX
OS   Hypocrea jecorina
OC   Eukaryota; Fungi; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Hypocreomycetidae; Hypocreales; Hypocreaceae; Hypocrea.
XX
RN   [1]
RP   1-289
RX   DOI; 10.1074/jbc.M107651200.
RX   MEDLINE; 21950703.
RX   PUBMED; 11825887.
RA   Chambergo F.S., Bonaccorsi E.D., Ferreira A.J.S., Ramos A.S.P.,
RA   Ferreira J.R.Jr., Abrahao-Neto J., Farah J.P.S., El-Dorry H.;
RT   "Elucidation of the metabolic fate of glucose in the filamentous fungus
RT   Trichoderma reesei using expressed sequence tag (EST) analysis and cDNA
RT   microarrays";
RL   J. Biol. Chem. 277(16):13983-13988(2002).
XX
DR   UNILIB; 24343; 9920.
XX
CC   On Feb 5, 2002 this sequence version replaced gi:18498149.
CC   Contact: El-Dorry, Hamza
CC   Department of Biochemistry
CC   Institute of Chemistry. University of Sao Paulo
CC   Avenida Professor Lineu Prestes, 748, Sao Paulo, SP, 05508-900,
CC   BRASIL
CC   Tel: (55) 11-38183848
CC   Fax: (55) 11-38183848
CC   Email: dorry@iq.usp.br
CC   PCR PRimers
CC   FORWARD: Universal M13 forward primer
CC   BACKWARD: Universal M13 reverse primer
CC   Plate: 39  row: A  column: 3
CC   Seq primer: M13 reverse primer
CC   High quality sequence stop: 287
CC   POLYA=No.
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..289
FT                   /db_xref="taxon:51453"
FT                   /db_xref="UNILIB:24343"
FT                   /mol_type="mRNA"
FT                   /note="Vector: pBluescript SK(+); Site_1: EcoRI; Site_2:
FT                   XhoI; anamorph=Trichoderma reesei; Cloned unidirectionally,
FT                   5' end of the cDNA cloned into EcoRI site of pBluescript.
FT                   Primer: Oligo (dT). Average insert size: 1,2 kb; Uni-ZAP XR
FT                   Vector system -5' adaptor sequence: 5'GAATTCGGCACGAG3' -3'
FT                   adaptor sequence: 5'CTCGAGTTTTTTTTTTTTTTTTTT3'"
FT                   /sex="Asexual"
FT                   /organism="Hypocrea jecorina"
FT                   /strain="QM9414 (ATCC26921)"
FT                   /clone="Tr-A3651"
FT                   /tissue_type="Mycelia"
FT                   /dev_stage="18 hr Glycerol-grown culture"
FT                   /lab_host="E. coli SOLR cells (kanamycin resistant)"
FT                   /clone_lib="TrEST-A"
XX
SQ   Sequence 289 BP; 68 A; 100 C; 79 G; 42 T; 0 other;
     gcacgagcca aagtcgacca aagccaaaca tgcgcatcac gctcaacgtc ttcaatcctc        60
     atggaggaga ccaggacagc ctggtgaccc tcgaggtctt ccccgacatg actgtcgcaa       120
     ccctgcgcga gtccgtccag gccgaggccg gcatcccgct cacgtcgcaa cacatctacc       180
     acaacggccg actcatctcg gacgatgcca agaccatgga gcagctgcag attggcgatg       240
     gcgacatgct ggccgtccac gtccgggaca tgagaggcag caccgggct                   289
//