ID   CF426680   standard; mRNA; EST; 665 BP.
XX
AC   CF426680;
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SV   CF426680.2
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DT   04-SEP-2003 (Rel. 77, Created)
DT   24-SEP-2003 (Rel. 77, Last updated, Version 2)
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DE   Lr_LC1ED_27B10_SAC Lumbricus rubellus Late Cocoon Library 1 Lumbricus
DE   rubellus cDNA clone Lr_LC1ED_27B10 5' similar to ref|ZP_00092693.1|
DE   hypothetical protein - Azotobacter vinelandii, mRNA sequence.
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KW   EST.
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OS   Lumbricus rubellus (humus earthworm)
OC   Eukaryota; Metazoa; Annelida; Clitellata; Oligochaeta; Haplotaxida;
OC   Lumbricina; Lumbricidae; Lumbricus.
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RN   [1]
RP   1-665
RA   Jones M., Chaseley J., Hedley B.A., Morgan J.C., Sturzenbaum S., Kille P.,
RA   Blaxter M.;
RT   "The Lumbricus rubellus EST program - Sequences from a cocoon library";
RL   Unpublished.
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DR   UNILIB; 33602; 14289.
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CC   On Sep 3, 2003 this sequence version replaced gi:34439381.
CC   Contact: Blaxter ML
CC   Institute of Cell, Animal and Population Biology
CC   University of Edinburgh
CC   Ashworth Labs, King's Buildings, West Mains Road, Edinburgh, EH9
CC   3JT, UK.
CC   Tel: +44 131 650 6760
CC   Fax: +44 131 670 5450
CC   Email: mark.blaxter@ed.ac.uk
CC   The library was prepared using protocols given by the supplier
CC   (Stratagene).
CC   PCR PRimers
CC   FORWARD: M13R(AGCGGATAACAATTTCACACAGGA)
CC   BACKWARD: T7PL(CTCACTATAGGGCGAATTGG)
CC   Plate: 27  row: B  column: 10
CC   Seq primer: SAC(GGGAACAAAAGCTGGAG)
CC   High quality sequence stop: 496.
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FH   Key             Location/Qualifiers
FH
FT   source          1..665
FT                   /db_xref="taxon:35632"
FT                   /db_xref="UNILIB:33602"
FT                   /mol_type="mRNA"
FT                   /note="Vector: pBluescript II SK+; The library was prepared
FT                   using protocols given by the supplier (Stratagene)."
FT                   /organism="Lumbricus rubellus"
FT                   /clone="Lr_LC1ED_27B10"
FT                   /tissue_type="Whole worm"
FT                   /dev_stage="Late Cocoon"
FT                   /clone_lib="Lumbricus rubellus Late Cocoon Library 1"
XX
SQ   Sequence 665 BP; 241 A; 117 C; 124 G; 183 T; 0 other;
     agcaaatcca gatcacacaa ataagaaatc atgccggact caccaaagca cgataagaag        60
     aagaaggatg gtcacaagaa gaaggaaggc agtggctcat cttccgattc tgaccatgac       120
     ggacacaaga aaaagcatgg tgattgccat ggaaaagaac atggaaaaga acatggaaaa       180
     aaggaatgtc atggacacaa gaaagatgat cataaacact agcagcgtct gagttagcga       240
     cacctgggga ctgatttctc ttccctctta aaggctacat gtgtcgtctc agccgagagt       300
     tgatgagagt catcgtcgac atcttcaacg tactgctgct tttgtccaat gaacagttgt       360
     tgttattttt attgctatat aagtaattca ctgcttttct acgctcaacc ttctacgact       420
     aggaaacaat aataataaga ccattttgta agtttttcaa atccaatgta ataaaattcg       480
     taaatttttt atatctatgg ccatgggcct agaagttaaa agaataatgg aacaaaatca       540
     atctccaatt gttttgaatc tgttcacgta ataatgttgg aaaaattctt cgatttctga       600
     acataatgaa tgaaaattaa actttgcaga gaatcctaaa caatttgtaa taaatactag       660
     ttata                                                                   665
//