ID   CF932767   standard; mRNA; EST; 622 BP.
XX
AC   CF932767;
XX
SV   CF932767.1
XX
DT   18-NOV-2003 (Rel. 77, Created)
DT   18-NOV-2003 (Rel. 77, Last updated, Version 1)
XX
DE   TrEST-B02F07.g2 TrEST-B Hypocrea jecorina cDNA clone Tr-B02F07.g2 5'
DE   similar to COG0625: Glutathione S-transferase [Azotobacter vinelandii],
DE   mRNA sequence.
XX
KW   EST.
XX
OS   Hypocrea jecorina
OC   Eukaryota; Fungi; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Hypocreomycetidae; Hypocreales; Hypocreaceae; Hypocrea.
XX
RN   [1]
RP   1-622
RA   Bonaccorsi E.D., Chambergo F.S., El-Dorry H.;
RT   "TrEST-B";
RL   Unpublished.
XX
DR   UNILIB; 33913; 14605.
XX
CC   Contact: El-Dorry, Hamza
CC   Department of Biochemistry
CC   Institute of Chemistry. University of Sao Paulo
CC   Avenida Professor Lineu Prestes, 748, Sao Paulo, SP, 05508-900,
CC   BRASIL
CC   Tel: (55) 11-38183848
CC   Fax: (55) 11-38183848
CC   Email: dorry@iq.usp.br
CC   PCR PRimers
CC   FORWARD: Universal M13 forward primer
CC   BACKWARD: Universal M13 reverse primer
CC   Plate: 02  row: F  column: 07
CC   Seq primer: M13 reverse primer
CC   High quality sequence stop: 433
CC   POLYA=No.
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..622
FT                   /db_xref="taxon:51453"
FT                   /db_xref="UNILIB:33913"
FT                   /mol_type="mRNA"
FT                   /note="Vector: pUC18; cDNA cloned into SmaI site of pUC18.
FT                   Average insert size: 1 kb"
FT                   /sex="Asexual"
FT                   /organism="Hypocrea jecorina"
FT                   /strain="QM9414 (ATCC26921)"
FT                   /clone="Tr-B02F07.g2"
FT                   /tissue_type="Mycelia"
FT                   /dev_stage="Mycelial culture grown from 100 mM and
FT                   harvested to 80 mM glicose concentrations."
FT                   /lab_host="E. coli DH10B cells (streptomycin resistant)"
FT                   /clone_lib="TrEST-B"
XX
SQ   Sequence 622 BP; 149 A; 229 C; 138 G; 106 T; 0 other;
     ccacgcgtcc gaaccgagac attatctcca cgggtagaat cagcagccat gggctctcag        60
     cagcaaatta ctctctttga cttgcccagc aaggagccca acaggacgtg gtctctgaat       120
     ccatggaaga cacgcctgct cttgaactac aagggcctcg actacaagac agaatggctc       180
     cactatcctg acattgcacc acgcctcaaa ccacacctcc cccccaacga aacaggcacg       240
     gcatacacca tccccgccat cctcttcccc gacggcacat acatgatgga ctctgccaag       300
     atcgcagccg ccatcgaaga gcgctaccct tctccccccg cccgcccctc gtcccccctc       360
     gtccctaaaa tcgaacacct caccctcgaa accatcacgc ccctcttccc catcctcatg       420
     cacaagctct cgcaagtcat cctgcccgac aaggactacg actactgggt cggccgacat       480
     gaacgccagc tgggcatgcc gctcgacgag tacgagcgcc agaacggtgg ccaaaaagtc       540
     ttggccgggg ctccggcggc gtttagccga gtgaccaact tctgaagaaa gacaagctga       600
     ggggccgttc ttcttgggcg ga                                                622
//