ID   CF933447   standard; mRNA; EST; 523 BP.
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AC   CF933447;
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SV   CF933447.1
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DT   18-NOV-2003 (Rel. 77, Created)
DT   18-NOV-2003 (Rel. 77, Last updated, Version 1)
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DE   TrEST-B15H10.g TrEST-B Hypocrea jecorina cDNA clone Tr-B15H10.g 5' similar
DE   to COG2175: Probable taurine catabolism dioxygenase [Azotobacter
DE   vinelandii], mRNA sequence.
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KW   EST.
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OS   Hypocrea jecorina
OC   Eukaryota; Fungi; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Hypocreomycetidae; Hypocreales; Hypocreaceae; Hypocrea.
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RN   [1]
RP   1-523
RA   Bonaccorsi E.D., Chambergo F.S., El-Dorry H.;
RT   "TrEST-B";
RL   Unpublished.
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DR   UNILIB; 33913; 14605.
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CC   Contact: El-Dorry, Hamza
CC   Department of Biochemistry
CC   Institute of Chemistry. University of Sao Paulo
CC   Avenida Professor Lineu Prestes, 748, Sao Paulo, SP, 05508-900,
CC   BRASIL
CC   Tel: (55) 11-38183848
CC   Fax: (55) 11-38183848
CC   Email: dorry@iq.usp.br
CC   PCR PRimers
CC   FORWARD: Universal M13 forward primer
CC   BACKWARD: Universal M13 reverse primer
CC   Plate: 15  row: H  column: 10
CC   Seq primer: M13 reverse primer
CC   High quality sequence start: 55
CC   High quality sequence stop: 493
CC   POLYA=No.
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FH   Key             Location/Qualifiers
FH
FT   source          1..523
FT                   /db_xref="taxon:51453"
FT                   /db_xref="UNILIB:33913"
FT                   /mol_type="mRNA"
FT                   /note="Vector: pUC18; cDNA cloned into SmaI site of pUC18.
FT                   Average insert size: 1 kb"
FT                   /sex="Asexual"
FT                   /organism="Hypocrea jecorina"
FT                   /strain="QM9414 (ATCC26921)"
FT                   /clone="Tr-B15H10.g"
FT                   /tissue_type="Mycelia"
FT                   /dev_stage="Mycelial culture grown from 100 mM and
FT                   harvested to 80 mM glicose concentrations."
FT                   /lab_host="E. coli DH10B cells (streptomycin resistant)"
FT                   /clone_lib="TrEST-B"
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SQ   Sequence 523 BP; 93 A; 176 C; 149 G; 92 T; 13 other;
     gggggnaggg ggggnnnnng gnnnnnncat gaatgaactc gctacggacc agcgtggtcg        60
     cggccgagtc tccccagtgg agtcaaggtc actgacctca ctccacacat tggctccgaa       120
     gtgagaggca tccggctgtc ctccctcagc gacgccggca aagacgagct ggctcacttc       180
     gtcgccacgc gcaaggtcgt ggccttccga gaccaggact tcgccgacct gcccattgcc       240
     gacgcgctca agtttggctc ctactttgga cgacaccaca tccatcccac gtctggctct       300
     cccgaaggct accccgagat ccacctcgtt caccgcggcg tcaacgacaa gggacatctg       360
     gacttcttca agacgaggac cagctctgtg gcctggcact cggacgtctc ttacgagcgg       420
     cagcctcccg ggacgacgtt cctttacatc ctggacaagc ccgaggtcgg cggtgacacc       480
     atcttcgtcg atgccgttca ggcgtacctg cccgggcggc cgn                         523
//