ID   CF943940   standard; mRNA; EST; 656 BP.
XX
AC   CF943940;
XX
SV   CF943940.1
XX
DT   20-NOV-2003 (Rel. 77, Created)
DT   20-NOV-2003 (Rel. 77, Last updated, Version 1)
XX
DE   TrEST-A0730 TrEST-A Hypocrea jecorina cDNA clone Tr-A0730 5' similar to
DE   COG0840: Methyl-accepting chemotaxis protein [Azotobacter vinelandii], mRNA
DE   sequence.
XX
KW   EST.
XX
OS   Hypocrea jecorina
OC   Eukaryota; Fungi; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Hypocreomycetidae; Hypocreales; Hypocreaceae; Hypocrea.
XX
RN   [1]
RP   1-656
RX   DOI; 10.1074/jbc.M107651200.
RX   MEDLINE; 21950703.
RX   PUBMED; 11825887.
RA   Chambergo F.S., Bonaccorsi E.D., Ferreira A.J.S., Ramos A.S.P.,
RA   Ferreira J.R.Jr., Abrahao-Neto J., Farah J.P.S., El-Dorry H.;
RT   "Elucidation of the metabolic fate of glucose in the filamentous fungus
RT   Trichoderma reesei using expressed sequence tag (EST) analysis and cDNA
RT   microarrays";
RL   J. Biol. Chem. 277(16):13983-13988(2002).
XX
DR   UNILIB; 24343; 9920.
XX
CC   Contact: El-Dorry, Hamza
CC   Department of Biochemistry
CC   Institute of Chemistry. University of Sao Paulo
CC   Avenida Professor Lineu Prestes, 748, Sao Paulo, SP, 05508-900,
CC   BRASIL
CC   Tel: (55) 11-38183848
CC   Fax: (55) 11-38183848
CC   Email: dorry@iq.usp.br
CC   PCR PRimers
CC   FORWARD: Universal M13 forward primer
CC   BACKWARD: Universal M13 reverse primer
CC   Plate: 8  row: E  column: 10
CC   Seq primer: M13 reverse primer
CC   High quality sequence stop: 466
CC   POLYA=No.
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..656
FT                   /db_xref="taxon:51453"
FT                   /db_xref="UNILIB:24343"
FT                   /mol_type="mRNA"
FT                   /note="Vector: pBluescript SK(+); Site_1: EcoRI; Site_2:
FT                   XhoI; anamorph=Trichoderma reesei; Cloned unidirectionally,
FT                   5' end of the cDNA cloned into EcoRI site of pBluescript.
FT                   Primer: Oligo (dT). Average insert size: 1,2 kb; Uni-ZAP XR
FT                   Vector system -5' adaptor sequence: 5'GAATTCGGCACGAG3' -3'
FT                   adaptor sequence: 5'CTCGAGTTTTTTTTTTTTTTTTTT3'"
FT                   /sex="Asexual"
FT                   /organism="Hypocrea jecorina"
FT                   /strain="QM9414 (ATCC26921)"
FT                   /clone="Tr-A0730"
FT                   /tissue_type="Mycelia"
FT                   /dev_stage="18 hr Glycerol-grown culture"
FT                   /lab_host="E. coli SOLR cells (kanamycin resistant)"
FT                   /clone_lib="TrEST-A"
XX
SQ   Sequence 656 BP; 196 A; 191 C; 132 G; 137 T; 0 other;
     ctgcacgcag ttcaccgagg gcaacaaaag aaagcttttc atacacataa aatcacacca        60
     aaaatcgcca tgaacccaaa cgcaaggccg ataaaacaca tccagcgcga ggatctttac       120
     accaacctcg aagttcgcat tcaatacctt cactcgttcc tcgacttttc aagtcgtgac       180
     attgacgccc tcatcagcga ctccaaatac atcaaagccc tcatccccgc cgtcgtaaat       240
     attgtttaca agaaactcct ccaatacgac attaccgccc gcgcattcac cacacgaagt       300
     acatcctttg aaggccctct cgatgacatt cccgacgaga acagccctca gattctgcac       360
     cgaaagatgt tcttgcgcgc gtatctgaat aaactgtgtt cggacccaag taagatggag       420
     ttttgggagt acctcgacaa agtcgggatg atgcacgtcg ggctcgggag gaaacaccct       480
     ctacacatcg aataccttca cctgggcgcc tgtctaggct tcatccagga catcatgaac       540
     gaagccatcc tggcccaccc gcgactggac attcagagaa aaaatcgcct cgtcaagggt       600
     tttaacaagg gtatctgggt ccaaaaggac cttatgggaa aatggcacgt taaaaa           656
//