ID   CK829321   standard; mRNA; EST; 392 BP.
XX
AC   CK829321;
XX
SV   CK829321.1
XX
DT   04-MAR-2004 (Rel. 79, Created)
DT   21-JUL-2004 (Rel. 80, Last updated, Version 3)
XX
DE   Fr_Fwd_10H08_T3 Forward subtracted cDNA library from fast skeletal muscle
DE   Takifugu rubripes cDNA clone Fr_Fwd_10H08 similar to ref|ZP_00092303.1|
DE   hypothetical protein - Azotobacter vinelandii. Score = 32.7 bits (73),
DE   Expect = 1.4, mRNA sequence.
XX
KW   EST.
XX
OS   Takifugu rubripes
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Euteleostei; Neoteleostei;
OC   Acanthomorpha; Acanthopterygii; Percomorpha; Tetraodontiformes;
OC   Tetradontoidea; Tetraodontidae; Takifugu.
XX
RN   [1]
RP   1-392
RA   Fernandes J., Kinghorn J., Elgar G., Suzuki Y., Watabe S., Johnston I.;
RT   "Differential gene expression in fast skeletal muscle from adult japanese
RT   pufferfish, Fugu rubripes";
RL   Unpublished.
XX
DR   UNILIB; 35439; 15433.
XX
CC   Contact: Jorge Fernandes
CC   Gatty Marine Laboratory
CC   University of St Andrews
CC   East Sands, St Andrews, Fife KY16 8LB, UK
CC   Tel: +44 1334 463449
CC   Fax: +44 1334 463443
CC   Email: jmof1@st-and.ac.uk
CC   PCR PRimers
CC   FORWARD: T3 short
CC   BACKWARD: T7 short
CC   Plate: 10  row: H  column: 08
CC   Seq primer: T3
CC   High quality sequence start: 7
CC   High quality sequence stop: 392
CC   POLYA=Yes.
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..392
FT                   /db_xref="taxon:31033"
FT                   /db_xref="UNILIB:35439"
FT                   /mol_type="mRNA"
FT                   /organism="Takifugu rubripes"
FT                   /clone="Fr_Fwd_10H08"
FT                   /tissue_type="fast skeletal muscle"
FT                   /clone_lib="Forward subtracted cDNA library from fast
FT                   skeletal muscle"
XX
SQ   Sequence 392 BP; 112 A; 85 C; 90 G; 105 T; 0 other;
     ccttagcgtg gtcgcggccg aggtaccaac tgaactggtt ggaaatggtt cagctttagt        60
     cttgaaccac caacagtaaa gtggatttga tcaccatatt cagcagtgtc gacctcagaa       120
     tctttaagat cagcattaga tccgatgaag aagccacccg tcaacatatt ggcctgcaga       180
     taatcaatca tcgattatca gcttcgggtg gatccggttt gtcccgtgac gagacctcgg       240
     atctctgcgc agcgtcattt tcagctacat gctttaaaga gctcttttga gaggtggtct       300
     gatcacttaa ggcagtatct gatattggtg gattccaata aacacgtatt ttggtcggtt       360
     ccaaatgaga ataacaacaa caaaaaaaaa gg                                     392
//