LOCUS CK829321 392 bp mRNA linear EST 15-NOV-2005 DEFINITION Fr_Fwd_10H08_T3 Forward subtracted cDNA library from fast skeletal muscle Takifugu rubripes cDNA clone Fr_Fwd_10H08 similar to ref|ZP_00092303.1| hypothetical protein - Azotobacter vinelandii. Score = 32.7 bits (73), Expect = 1.4, mRNA sequence. ACCESSION CK829321 VERSION CK829321.1 GI:44901862 KEYWORDS EST. SOURCE Takifugu rubripes (Fugu rubripes) ORGANISM Takifugu rubripes Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Actinopterygii; Neopterygii; Teleostei; Euteleostei; Neoteleostei; Acanthomorpha; Acanthopterygii; Percomorpha; Tetraodontiformes; Tetradontoidea; Tetraodontidae; Takifugu. REFERENCE 1 (bases 1 to 392) AUTHORS Fernandes,J.M., Mackenzie,M.G., Elgar,G., Suzuki,Y., Watabe,S., Kinghorn,J.R. and Johnston,I.A. TITLE A genomic approach to reveal novel genes associated with myotube formation in the model teleost, Takifugu rubripes JOURNAL Physiol. Genomics 22 (3), 327-338 (2005) PUBMED 15928209 COMMENT Contact: Jorge Fernandes Gatty Marine Laboratory University of St Andrews East Sands, St Andrews, Fife KY16 8LB, UK Tel: +44 1334 463449 Fax: +44 1334 463443 Email: jmof1@st-and.ac.uk PCR PRimers FORWARD: T3 short BACKWARD: T7 short Plate: 10 row: H column: 08 Seq primer: T3 High quality sequence start: 7 High quality sequence stop: 392 POLYA=Yes. FEATURES Location/Qualifiers source 1..392 /organism="Takifugu rubripes" /mol_type="mRNA" /db_xref="taxon:31033" /clone="Fr_Fwd_10H08" /tissue_type="fast skeletal muscle" /clone_lib="Forward subtracted cDNA library from fast skeletal muscle" ORIGIN 1 ccttagcgtg gtcgcggccg aggtaccaac tgaactggtt ggaaatggtt cagctttagt 61 cttgaaccac caacagtaaa gtggatttga tcaccatatt cagcagtgtc gacctcagaa 121 tctttaagat cagcattaga tccgatgaag aagccacccg tcaacatatt ggcctgcaga 181 taatcaatca tcgattatca gcttcgggtg gatccggttt gtcccgtgac gagacctcgg 241 atctctgcgc agcgtcattt tcagctacat gctttaaaga gctcttttga gaggtggtct 301 gatcacttaa ggcagtatct gatattggtg gattccaata aacacgtatt ttggtcggtt 361 ccaaatgaga ataacaacaa caaaaaaaaa gg //