ID   CN854424   standard; mRNA; EST; 564 BP.
XX
AC   CN854424;
XX
SV   CN854424.1
XX
DT   04-JUN-2004 (Rel. 80, Created)
DT   04-JUN-2004 (Rel. 80, Last updated, Version 1)
XX
DE   Ha_mx0_25a01_SP6 Lobster Multiple Tissues, Normalized Homarus americanus
DE   cDNA clone Ha_mx0_25a01 5' similar to ref|ZP_00088450.1| COG0183:
DE   Acetyl-CoA acetyltransferase - Azotobacter vinelandii. Score = 41.2 bits
DE   (95), Expect = 0.012, mRNA sequence.
XX
KW   EST.
XX
OS   Homarus americanus (American lobster)
OC   Eukaryota; Metazoa; Arthropoda; Crustacea; Malacostraca; Eumalacostraca;
OC   Eucarida; Decapoda; Pleocyemata; Astacidea; Nephropoidea; Nephropidae;
OC   Homarus.
XX
RN   [1]
RP   1-564
RA   Towle D.W., Smith C.M.;
RT   "Expressed sequence tags in a normalized cDNA library prepared from
RT   multiple tissues of adult intermolt American lobster, Homarus americanus";
RL   Unpublished.
XX
DR   UNILIB; 35851; 15831.
XX
CC   Contact: David W. Towle
CC   Marine DNA Sequencing and Analysis Center
CC   Mount Desert Island Biological Laboratory
CC   Old Bar Harbor Road, Salsbury Cove, ME 04672 USA
CC   Tel: 207-288-9880 x474
CC   Fax: 207-288-2130
CC   Email: dtowle@mdibl.org
CC   Plate: 25  row: a  column: 01
CC   Seq primer: SP6
CC   High quality sequence stop: 500.
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..564
FT                   /db_xref="taxon:6706"
FT                   /db_xref="UNILIB:35851"
FT                   /mol_type="mRNA"
FT                   /note="Vector: pCMV Sport 6.1; Total RNA samples were
FT                   prepared individually from each tissue, checked for
FT                   quality, then pooled for construction and normalization of
FT                   cDNA library by Invitrogen. Plasmids were isolated and
FT                   inserts end-sequenced by the Marine DNA Sequencing and
FT                   Analysis Facility at Mount Desert Island Biological
FT                   Laboratory. Traces were processed for submission to dbEST
FT                   by trace2dbest software (Parkinson, Anthony and Blaxter,
FT                   unpublished software)."
FT                   /organism="Homarus americanus"
FT                   /clone="Ha_mx0_25a01"
FT                   /tissue_type="Gill, epipodite, branchiostegite, heart,
FT                   ovary, testis, antennal gland, abdominal muscle,
FT                   hepatopancreas, brain"
FT                   /dev_stage="Adult intermolt"
FT                   /clone_lib="Lobster Multiple Tissues, Normalized"
XX
SQ   Sequence 564 BP; 138 A; 144 C; 157 G; 125 T; 0 other;
     ccaacaccgt acttatttat tcctcatttc ctctatcctg tgattaatcg tctagcgtta        60
     agtgtttaaa gtggccgtga ttttaaagtg taaatgtaaa gcgtggaggt gtggaggtgt       120
     gtaggtgtgt gccagtgtca gccacggcaa gtgtgaggcc caagaagtag tgtatgtttt       180
     cttcgtgaca aggtgcggca ctatcccacc tagttaagcc acccagtcag cgtcaaccag       240
     ccagtcagcc aacctttcat tcctaataac cgcacagatg cccacagcac acggcagcca       300
     agaggtactg gactggaagg acgtgtggta cctcctggag gaagggcggg gcaggatcga       360
     tctcgacttt ttcgaggaac ggatccccag gacgcccaac aacgcccagg ccatcccaca       420
     agcgctgaag ctactgcagg aggccgacgt ccggggcaat ggctacgtcg agtacaacga       480
     attcagacac ttctacgaca cagtgttgcc agttaactcg tgagagagag gcgtcctgat       540
     gcgggtggcc ttagacgtgc tgga                                              564
//