ID   CN949948   standard; mRNA; EST; 624 BP.
XX
AC   CN949948;
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SV   CN949948.1
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DT   10-JUN-2004 (Rel. 80, Created)
DT   10-JUN-2004 (Rel. 80, Last updated, Version 1)
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DE   Ha_mx0_28h04_SP6 Lobster Multiple Tissues, Normalized Homarus americanus
DE   cDNA clone Ha_mx0_28h04 5' similar to ref|ZP_00092199.1| COG0500:
DE   SAM-dependent methyltransferases - Azotobacter vinelandii. Score = 84.3
DE   bits (207), Expect = 2e-15, mRNA sequence.
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KW   EST.
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OS   Homarus americanus (American lobster)
OC   Eukaryota; Metazoa; Arthropoda; Crustacea; Malacostraca; Eumalacostraca;
OC   Eucarida; Decapoda; Pleocyemata; Astacidea; Nephropoidea; Nephropidae;
OC   Homarus.
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RN   [1]
RP   1-624
RA   Towle D.W., Smith C.M.;
RT   "Expressed sequence tags in a normalized cDNA library prepared from
RT   multiple tissues of adult intermolt American lobster, Homarus americanus";
RL   Unpublished.
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DR   UNILIB; 35851; 15831.
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CC   Contact: David W. Towle
CC   Marine DNA Sequencing and Analysis Center
CC   Mount Desert Island Biological Laboratory
CC   Old Bar Harbor Road, Salsbury Cove, ME 04672 USA
CC   Tel: 207-288-9880 x474
CC   Fax: 207-288-2130
CC   Email: dtowle@mdibl.org
CC   Plate: 28  row: h  column: 04
CC   Seq primer: SP6
CC   High quality sequence stop: 487.
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FH   Key             Location/Qualifiers
FH
FT   source          1..624
FT                   /db_xref="taxon:6706"
FT                   /db_xref="UNILIB:35851"
FT                   /mol_type="mRNA"
FT                   /note="Vector: pCMV Sport 6.1; Total RNA samples were
FT                   prepared individually from each tissue, checked for
FT                   quality, then pooled for construction and normalization of
FT                   cDNA library by Invitrogen. Plasmids were isolated and
FT                   inserts end-sequenced by the Marine DNA Sequencing and
FT                   Analysis Facility at Mount Desert Island Biological
FT                   Laboratory. Traces were processed for submission to dbEST
FT                   by trace2dbest software (Parkinson, Anthony and Blaxter,
FT                   unpublished software)."
FT                   /organism="Homarus americanus"
FT                   /clone="Ha_mx0_28h04"
FT                   /tissue_type="Gill, epipodite, branchiostegite, heart,
FT                   ovary, testis, antennal gland, abdominal muscle,
FT                   hepatopancreas, brain"
FT                   /dev_stage="Adult intermolt"
FT                   /clone_lib="Lobster Multiple Tissues, Normalized"
XX
SQ   Sequence 624 BP; 168 A; 121 C; 167 G; 168 T; 0 other;
     cttgagagtg gaaggatgtc tcaatttgtc agtggtggat accttttcaa ataaggttta        60
     ggtacggggt cactaaacat ggataaacca gtggacatgg tgtttaataa tgctggatta       120
     gtgaagaaat acgccgagtt tcgccctgtt cctccacgct ctctcgtcaa tacagttgtc       180
     gactatgtga aggaaaaagt ggatcccctg caggtgtgtg tggacattgg gtgtgggtca       240
     ggacaaaaca ccaggctgta cagtgaacat ttcaagcaag tcatcggtct ggatgtcagt       300
     ctggagcagc tcagagtagc tgaacaagac catctacatc agaatgttgt ttacaaggag       360
     gggttagctg agcacatgcc tgttgagaat gcatcggtgg atctggtgac gagttgtgca       420
     gcggtccatt ggttcaactt agataccttc tataaagagg tagacagaga gttgcgaccg       480
     gggggagtac tggcttgtta cagttacctg gcttgtacac ctctttatgc tggcaaaaac       540
     ttaaggtcta caatattaga gatgcattca agcttggacc ccaactggtc aatgggacat       600
     caccatttaa actctgatta tgcc                                              624
//