ID   CN952032   standard; mRNA; EST; 581 BP.
XX
AC   CN952032;
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SV   CN952032.1
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DT   10-JUN-2004 (Rel. 80, Created)
DT   10-JUN-2004 (Rel. 80, Last updated, Version 1)
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DE   Ha_mx0_55a09_SP6 Lobster Multiple Tissues, Normalized Homarus americanus
DE   cDNA clone Ha_mx0_55a09 5' similar to ref|ZP_00092227.1| COG3180: Putative
DE   ammonia monooxygenase - Azotobacter vinelandii. Score = 35.4 bits (80),
DE   Expect = 0.70, mRNA sequence.
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KW   EST.
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OS   Homarus americanus (American lobster)
OC   Eukaryota; Metazoa; Arthropoda; Crustacea; Malacostraca; Eumalacostraca;
OC   Eucarida; Decapoda; Pleocyemata; Astacidea; Nephropoidea; Nephropidae;
OC   Homarus.
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RN   [1]
RP   1-581
RA   Towle D.W., Smith C.M.;
RT   "Expressed sequence tags in a normalized cDNA library prepared from
RT   multiple tissues of adult intermolt American lobster, Homarus americanus";
RL   Unpublished.
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DR   UNILIB; 35851; 15831.
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CC   Contact: David W. Towle
CC   Marine DNA Sequencing and Analysis Center
CC   Mount Desert Island Biological Laboratory
CC   Old Bar Harbor Road, Salsbury Cove, ME 04672 USA
CC   Tel: 207-288-9880 x474
CC   Fax: 207-288-2130
CC   Email: dtowle@mdibl.org
CC   Plate: 55  row: a  column: 09
CC   Seq primer: SP6
CC   High quality sequence stop: 507.
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FH   Key             Location/Qualifiers
FH
FT   source          1..581
FT                   /db_xref="taxon:6706"
FT                   /db_xref="UNILIB:35851"
FT                   /mol_type="mRNA"
FT                   /note="Vector: pCMV Sport 6.1; Total RNA samples were
FT                   prepared individually from each tissue, checked for
FT                   quality, then pooled for construction and normalization of
FT                   cDNA library by Invitrogen. Plasmids were isolated and
FT                   inserts end-sequenced by the Marine DNA Sequencing and
FT                   Analysis Facility at Mount Desert Island Biological
FT                   Laboratory. Traces were processed for submission to dbEST
FT                   by trace2dbest software (Parkinson, Anthony and Blaxter,
FT                   unpublished software)."
FT                   /organism="Homarus americanus"
FT                   /clone="Ha_mx0_55a09"
FT                   /tissue_type="Gill, epipodite, branchiostegite, heart,
FT                   ovary, testis, antennal gland, abdominal muscle,
FT                   hepatopancreas, brain"
FT                   /dev_stage="Adult intermolt"
FT                   /clone_lib="Lobster Multiple Tissues, Normalized"
XX
SQ   Sequence 581 BP; 128 A; 166 C; 151 G; 136 T; 0 other;
     accggtcgct gcaccacttc ctacccctga tgctgttgtc cagtctgctg ccctaccctg        60
     tcacttacct gcctgctgac gcagctgata ttccgccttg gtctgacctt cgcggtccat       120
     cacctagtct gcctgattcg gtggttgatc atcgtgatcc gccacctgac ctggtcgacc       180
     cactacctaa tcaaatcgcc ccaccacttg atttaagcga tccattatcc actcaggact       240
     ctttgttccc tggcacaagc gaatctcccg tagcgtggga gcgtctggac tcgtgtagaa       300
     gtccgtcaca ggtagtggag cgacaggtgg cctgggcgac gagggtcatc gacaaacaga       360
     aggaagtgat caggaacctt cagatgagat tcgtagcact ggagaccaaa attgatagaa       420
     tgttcggatg ctgtaccaag cagccaccta caccctgacc accgctgaag cttggacgtg       480
     gttgtgcagc tggtgaggga tggaaggtag ctgacgtacc ctggtggtga gtggactgaa       540
     ctactgctac tgtcccaggc aagaagaaaa gctctacctt t                           581
//