ID   CV021969   standard; mRNA; EST; 515 BP.
XX
AC   CV021969;
XX
SV   CV021969.1
XX
DT   20-AUG-2004 (Rel. 80, Created)
DT   20-AUG-2004 (Rel. 80, Last updated, Version 1)
XX
DE   CS_gil_21H08_M13Reverse Blue crab gill, normalized Callinectes sapidus cDNA
DE   clone CS_gil_21H08 5' similar to ref|ZP_00090884.1| COG0500: SAM-dependent
DE   methyltransferases - Azotobacter vinelandii. Score = 32.3 bits (72), Expect
DE   = 4.7, mRNA sequence.
XX
KW   EST.
XX
OS   Callinectes sapidus (blue crab)
OC   Eukaryota; Metazoa; Arthropoda; Crustacea; Malacostraca; Eumalacostraca;
OC   Eucarida; Decapoda; Pleocyemata; Brachyura; Eubrachyura; Portunoidea;
OC   Portunidae; Callinectes.
XX
RN   [1]
RP   1-515
RA   Shafer T.H., Coblentz F.E., Towle D.W.;
RT   "Expressed sequence tags from normalized cDNA libraries prepared from gill
RT   and hypodermis tissues of the blue crab, Callinectes sapidus";
RL   Unpublished.
XX
DR   UNILIB; 37905; 16215.
XX
CC   Contact: Thomas H. Shafer
CC   Department of Biological Sciences
CC   University of North Carolina Wilmington
CC   601 S. College Rd, Wilmington, NC 28403, USA
CC   Tel: 910-962-7275
CC   Fax: 910-962-4066
CC   Email: shafert@uncw.edu
CC   Plate: 21  row: H  column: 08
CC   Seq primer: M13 Reverse
CC   High quality sequence stop: 488.
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..515
FT                   /db_xref="taxon:6763"
FT                   /db_xref="UNILIB:37905"
FT                   /mol_type="mRNA"
FT                   /note="Vector: pCMV Sport 6.1; Total RNA samples were
FT                   prepared individually from each tissue, checked for
FT                   quality, and then pooled for construction and normalization
FT                   of a cDNA library by Invitrogen. Plasmids were isolated and
FT                   inserts sequenced from their 5'-ends by the Blue Crab
FT                   Molecular Genetics Laboratory at the University of North
FT                   Carolina Wilmington. Traces were trimmed, compared (BLASTx)
FT                   to NCBI non-redundant protein database as of 19 July 2004,
FT                   and processed for submission to dbEST by trace2dbEST
FT                   software (Parkinson, Anthony and Blaxter, unpublished
FT                   software)."
FT                   /organism="Callinectes sapidus"
FT                   /clone="CS_gil_21H08"
FT                   /tissue_type="Pooled anterior and posterior gills from
FT                   crabs acclimated to salinities of 35 and 5 parts per
FT                   thousand"
FT                   /dev_stage="Adult intermolt"
FT                   /clone_lib="Blue crab gill, normalized"
XX
SQ   Sequence 515 BP; 122 A; 118 C; 155 G; 120 T; 0 other;
     ctggggcgct gacagccttc gtgacagtgg taggacacgg ggggtggctg aggtggcagt        60
     gtcacggctc gctagtggca cacacaccca gtggttgggg gcgtccgggg cgccgtgcct       120
     ggccgtcccc tgcccacagt ctggttttgc tggcttgcca ctcgccctgc atagaaaaat       180
     ggtgtgtagg atataactag ggcacaggaa aattgtgagg atgcaggaaa tagcacttgt       240
     tacgcagtga agggatgatg ctctactgct gtgaatagct cgaggggagt gttgagtgtc       300
     aagttgaaac agaaaccatt ctagaaacca tggagacagg tgggtgcggc acacagtgaa       360
     cccctgctgg ccaccttgtg accagatgat ctcagccgac gtacttaggt cacaaagtca       420
     gtcgctttgt aacatgtcaa ccatcggctg acatgccatc cttgcagata tgtacatatt       480
     gttgtcattt caaatgcaat aaaatagttt cggtg                                  515
//