ID   CV161726   standard; mRNA; EST; 542 BP.
XX
AC   CV161726;
XX
SV   CV161726.1
XX
DT   09-SEP-2004 (Rel. 81, Created)
DT   09-SEP-2004 (Rel. 81, Last updated, Version 1)
XX
DE   CS_gil_51G09_M13Reverse Blue crab gill, normalized Callinectes sapidus cDNA
DE   clone CS_gil_51G09 5' similar to ref|ZP_00091118.1| COG0210: Superfamily I
DE   DNA and RNA helicases [Azotobacter. Score = 39.7 bits (91), Expect = 0.034,
DE   mRNA sequence.
XX
KW   EST.
XX
OS   Callinectes sapidus (blue crab)
OC   Eukaryota; Metazoa; Arthropoda; Crustacea; Malacostraca; Eumalacostraca;
OC   Eucarida; Decapoda; Pleocyemata; Brachyura; Eubrachyura; Portunoidea;
OC   Portunidae; Callinectes.
XX
RN   [1]
RP   1-542
RA   Shafer T.H., Coblentz F.E., Towle D.W.;
RT   "Expressed sequence tags from normalized cDNA libraries prepared from gill
RT   and hypodermis tissues of the blue crab, Callinectes sapidus";
RL   Unpublished.
XX
DR   UNILIB; 37905; 16215.
XX
CC   Contact: Thomas H. Shafer
CC   Department of Biological Sciences
CC   University of North Carolina Wilmington
CC   601 S. College Rd, Wilmington, NC 28403, USA
CC   Tel: 910-962-7275
CC   Fax: 910-962-4066
CC   Email: shafert@uncw.edu
CC   Plate: 51  row: G  column: 09
CC   Seq primer: M13 Reverse
CC   High quality sequence stop: 420.
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..542
FT                   /db_xref="taxon:6763"
FT                   /db_xref="UNILIB:37905"
FT                   /mol_type="mRNA"
FT                   /note="Vector: pCMV Sport 6.1; Total RNA samples were
FT                   prepared individually from each tissue, checked for
FT                   quality, and then pooled for construction and normalization
FT                   of a cDNA library by Invitrogen. Plasmids were isolated and
FT                   inserts sequenced from their 5'-ends by the Blue Crab
FT                   Molecular Genetics Laboratory at the University of North
FT                   Carolina Wilmington. Traces were trimmed, compared (BLASTx)
FT                   to NCBI non-redundant protein database as of 19 July 2004,
FT                   and processed for submission to dbEST by trace2dbEST
FT                   software (Parkinson, Anthony and Blaxter, unpublished
FT                   software)."
FT                   /organism="Callinectes sapidus"
FT                   /clone="CS_gil_51G09"
FT                   /tissue_type="Pooled anterior and posterior gills from
FT                   crabs acclimated to salinities of 35 and 5 parts per
FT                   thousand"
FT                   /dev_stage="Adult intermolt"
FT                   /clone_lib="Blue crab gill, normalized"
XX
SQ   Sequence 542 BP; 75 A; 209 C; 182 G; 76 T; 0 other;
     cccggctcca cctgtgccac cctttgtccc tgctgcaccc ccgcccctct ctgccccctg        60
     gccgccgcct ctcccaccgt gccaccctcg cccgctgccc agcccgtccc tgtcaccgcc       120
     ctgggtcttg gcgagggggc ggatgttgcc accctgagcc gcagcctggg tgacctgagt       180
     cggccacagg agagcagtgc ggcagggcgc agcatgacct tcagcggcag cttggaccgt       240
     cacacgccac gctcaggccc gcagcgtccc tacggccgcc gctgcaagag cacggcgcac       300
     attgtgctgc agaacaacga cactgtgaag gagccgcgcc cccacgaggt gcacgccgcc       360
     tcgggtacac acctgcacgt gccctcgggt gaggcacgag ggcgtgccag gcaccggacc       420
     gcccctgcgg cagggagggg agagaggggc ggctggcacc gggtggaggg tgtagggcga       480
     ggcgcgtggg tgggggccag cccctgctgc tcatgccacc attgggctcc tctgtgccca       540
     tc                                                                      542
//