ID   CV161785   standard; mRNA; EST; 666 BP.
XX
AC   CV161785;
XX
SV   CV161785.1
XX
DT   09-SEP-2004 (Rel. 81, Created)
DT   09-SEP-2004 (Rel. 81, Last updated, Version 1)
XX
DE   CS_gil_48D04_M13Reverse Blue crab gill, normalized Callinectes sapidus cDNA
DE   clone CS_gil_48D04 5' similar to ref|ZP_00092593.1| COG0469: Pyruvate
DE   kinase - Azotobacter vinelandii. Score = 34.3 bits (77), Expect = 2.2, mRNA
DE   sequence.
XX
KW   EST.
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OS   Callinectes sapidus (blue crab)
OC   Eukaryota; Metazoa; Arthropoda; Crustacea; Malacostraca; Eumalacostraca;
OC   Eucarida; Decapoda; Pleocyemata; Brachyura; Eubrachyura; Portunoidea;
OC   Portunidae; Callinectes.
XX
RN   [1]
RP   1-666
RA   Shafer T.H., Coblentz F.E., Towle D.W.;
RT   "Expressed sequence tags from normalized cDNA libraries prepared from gill
RT   and hypodermis tissues of the blue crab, Callinectes sapidus";
RL   Unpublished.
XX
DR   UNILIB; 37905; 16215.
XX
CC   Contact: Thomas H. Shafer
CC   Department of Biological Sciences
CC   University of North Carolina Wilmington
CC   601 S. College Rd, Wilmington, NC 28403, USA
CC   Tel: 910-962-7275
CC   Fax: 910-962-4066
CC   Email: shafert@uncw.edu
CC   Plate: 48  row: D  column: 04
CC   Seq primer: M13 Reverse
CC   High quality sequence stop: 504.
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..666
FT                   /db_xref="taxon:6763"
FT                   /db_xref="UNILIB:37905"
FT                   /mol_type="mRNA"
FT                   /note="Vector: pCMV Sport 6.1; Total RNA samples were
FT                   prepared individually from each tissue, checked for
FT                   quality, and then pooled for construction and normalization
FT                   of a cDNA library by Invitrogen. Plasmids were isolated and
FT                   inserts sequenced from their 5'-ends by the Blue Crab
FT                   Molecular Genetics Laboratory at the University of North
FT                   Carolina Wilmington. Traces were trimmed, compared (BLASTx)
FT                   to NCBI non-redundant protein database as of 19 July 2004,
FT                   and processed for submission to dbEST by trace2dbEST
FT                   software (Parkinson, Anthony and Blaxter, unpublished
FT                   software)."
FT                   /organism="Callinectes sapidus"
FT                   /clone="CS_gil_48D04"
FT                   /tissue_type="Pooled anterior and posterior gills from
FT                   crabs acclimated to salinities of 35 and 5 parts per
FT                   thousand"
FT                   /dev_stage="Adult intermolt"
FT                   /clone_lib="Blue crab gill, normalized"
XX
SQ   Sequence 666 BP; 141 A; 147 C; 203 G; 175 T; 0 other;
     acaagccggt gtccttaagt gattcttagc gagcaagtgt ttattacgtg ggtaccttga        60
     tagcgtcgcc atagacccat agagggtgtg taggaaaggt tagggagaga gagagagagc       120
     tagtgagtgg tgatgataat gagtggcaga gatccactcc ctggcccttt gctggatgga       180
     gtacatggca aggcatcagt ggtctttatt tattagcgtg tggtcctgcc tagcctacgg       240
     cagtcttggc acaccaccac caccaccacc gggcaggcag gccggccaga ctagccattt       300
     gtgatgattg ttggtgattc agtagttcta tttagtggga ggtctgcaac tactttgtat       360
     attttatccc taatcatata tatttttgct cctttagtta ggtataggat actgtcggga       420
     ataaaaatga attaccgcag atgaaactta ggaaaattga acattccgtg tgtatgtggc       480
     atgcgcgtgg ccccggagcg tggcacgtcg ggctatgcgt cgcgtcatac ccctggcaag       540
     gcgtggctca gtgccgtgtg ttgccagagg aatggcgtgg cctgcggggg acacgcccgc       600
     accggggcgg cgcgacgtgc agtgtgccgt gctttcatct ccctgtcctg tggatggctc       660
     tggttg                                                                  666
//