ID   CV162199   standard; mRNA; EST; 649 BP.
XX
AC   CV162199;
XX
SV   CV162199.1
XX
DT   09-SEP-2004 (Rel. 81, Created)
DT   09-SEP-2004 (Rel. 81, Last updated, Version 1)
XX
DE   CS_hyp_04f08_M13Reverse Blue crab hypodermis, normalized Callinectes
DE   sapidus cDNA clone CS_hyp_04f08 5' similar to ref|ZP_00090884.1| COG0500:
DE   SAM-dependent methyltransferases - Azotobacter vinelandii. Score = 35.0
DE   bits (79), Expect = 1.2, mRNA sequence.
XX
KW   EST.
XX
OS   Callinectes sapidus (blue crab)
OC   Eukaryota; Metazoa; Arthropoda; Crustacea; Malacostraca; Eumalacostraca;
OC   Eucarida; Decapoda; Pleocyemata; Brachyura; Eubrachyura; Portunoidea;
OC   Portunidae; Callinectes.
XX
RN   [1]
RP   1-649
RA   Shafer T.H., Coblentz F.E., Towle D.W.;
RT   "Expressed sequence tags from normalized cDNA libraries prepared from gill
RT   and hypodermis tissues of the blue crab, Callinectes sapidus";
RL   Unpublished.
XX
DR   UNILIB; 38039; 16351.
XX
CC   Contact: Thomas H. Shafer
CC   Department of Biological Sciences
CC   University of North Carolina Wilmington
CC   601 S. College Rd, Wilmington, NC 28403, USA
CC   Tel: 910-962-7275
CC   Fax: 910-962-4066
CC   Email: shafert@uncw.edu
CC   Plate: 04  row: f  column: 08
CC   Seq primer: M13 Reverse
CC   High quality sequence start: 5
CC   High quality sequence stop: 524.
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..649
FT                   /db_xref="taxon:6763"
FT                   /db_xref="UNILIB:38039"
FT                   /mol_type="mRNA"
FT                   /note="Vector: pCMV Sport 6.1; Total RNA samples were
FT                   prepared individually from each tissue, checked for
FT                   quality, and then pooled for construction and normalization
FT                   of a cDNA library by Invitrogen. Plasmids were isolated and
FT                   inserts sequenced from their 5'-ends by the Blue Crab
FT                   Molecular Genetics Laboratory at the University of North
FT                   Carolina Wilmington. Traces were trimmed, compared (BLASTx)
FT                   to NCBI non-redundant protein database as of 19 July 2004,
FT                   and processed for submission to dbEST by trace2dbEST
FT                   software (Parkinson, Anthony and Blaxter, unpublished
FT                   software)."
FT                   /organism="Callinectes sapidus"
FT                   /clone="CS_hyp_04f08"
FT                   /tissue_type="Pooled hypodermal epithelium from the
FT                   mid-dorsal region and arthrodial membrane of premolt (stage
FT                   D2) and 3-hour postmolt crabs"
FT                   /dev_stage="Adult"
FT                   /clone_lib="Blue crab hypodermis, normalized"
XX
SQ   Sequence 649 BP; 157 A; 223 C; 147 G; 122 T; 0 other;
     agataaatct ttaaacgaaa aaaacattct cagtatcctt cacatcctct cagcaattct        60
     ctcatgccta cagatgcgct acgtgtttct gctggcggcg ctggtggtgg gcgtcgcgtg       120
     gggccgccct tcagagactc tgcagctctc caccacgccc cccacggcta cgatgccaac       180
     ggtgcagctc atcaagaacc tgggaaacct caagatccac gtgacacagc agcctgaagg       240
     ggactacggg ctggaagtgt acgacaacaa actggagaag ctggtgccct ccacggtctt       300
     ctcactgcct ggagactgga aaccccccgg cctggagaag tcccgcattg tgcacgctga       360
     gttcctggac agcggggatg tggtggtggc cttcttcagc ctcgagaaca gcctggacct       420
     catcctgttg cccaaggaac tattcgtcta caagaaaaca aactcaggcg tgattgtctt       480
     cccgaagctt gatgtccctt ggcaccccac tgtgtccacg ccgaccgagt actggaaatg       540
     gcctgacacc accacaccct acaccaccac caccaccacc acagcggcct acaccactac       600
     accctacact accacaccct acaccaccac cacagcggcc tacaccact                   649
//