ID   CV221420   standard; mRNA; EST; 571 BP.
XX
AC   CV221420;
XX
SV   CV221420.1
XX
DT   17-SEP-2004 (Rel. 81, Created)
DT   17-SEP-2004 (Rel. 81, Last updated, Version 1)
XX
DE   Le_mx0_48b04_SP6 Little Skate Multiple Tissues, Normalized Leucoraja
DE   erinacea cDNA clone Le_mx0_48b04 5' similar to ref|ZP_00092437.1| COG1981:
DE   Predicted membrane protein - Azotobacter vinelandii. Score = 33.1 bits
DE   (74), Expect = 3.7, mRNA sequence.
XX
KW   EST.
XX
OS   Leucoraja erinacea (little skate)
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Chondrichthyes;
OC   Elasmobranchii; Squalea; Hypnosqualea; Pristiorajea; Batoidea; Rajiformes;
OC   Rajidae; Leucoraja.
XX
RN   [1]
RP   1-571
RA   Towle D.W., Smith C.M.;
RT   "Expressed sequence tags in a normalized cDNA library prepared from
RT   multiple tissues of adult little skate, Leucoraja erinacea";
RL   Unpublished.
XX
DR   UNILIB; 35911; 15890.
XX
CC   Contact: David W. Towle
CC   Marine DNA Sequencing and Analysis Center
CC   Mount Desert Island Biological Laboratory
CC   Old Bar Harbor Road, Salsbury Cove, ME 04672 USA
CC   Tel: 207-288-9880 x474
CC   Fax: 207-288-2130
CC   Email: dtowle@mdibl.org
CC   Plate: 48  row: b  column: 04
CC   Seq primer: SP6
CC   High quality sequence stop: 503.
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..571
FT                   /db_xref="taxon:7782"
FT                   /db_xref="UNILIB:35911"
FT                   /mol_type="mRNA"
FT                   /note="Vector: pCMV Sport 6.1; Total RNA samples were
FT                   prepared individually from each tissue, checked for
FT                   quality, then pooled for construction and normalization of
FT                   cDNA library by Invitrogen. Plasmids were isolated and
FT                   inserts end-sequenced by the Marine DNA Sequencing and
FT                   Analysis Facility at Mount Desert Island Biological
FT                   Laboratory. Traces were processed for submission to dbEST
FT                   by trace2dbest software (Parkinson, Anthony and Blaxter,
FT                   unpublished software)."
FT                   /organism="Leucoraja erinacea"
FT                   /clone="Le_mx0_48b04"
FT                   /tissue_type="Liver, kidney, brain, testis, ovary, gill,
FT                   heart, spleen, rectal gland"
FT                   /dev_stage="Adult"
FT                   /clone_lib="Little Skate Multiple Tissues, Normalized"
XX
SQ   Sequence 571 BP; 157 A; 99 C; 141 G; 174 T; 0 other;
     tagtccatct gcacttgaag cacttgccga gagaactatc gtgagcatga tttgtaagtg        60
     tgttgtagaa tggttaaaga ttgtagagtg ctctactact ggtaaaaagc ccaagactta       120
     atttctagcc tgtgctggtt tggtggggta gtagtttatg gtgaactctg gtttgacgca       180
     tttatgtttg cacaagttgc ccgcaattgg caccgcaggt tttcgcttaa aattgccgct       240
     cctgcatcag cactttcagt acatagtggg aagaaattga aaattctaga ttgggtgatc       300
     acagttatca ttttaaaagt taatgacaca gtttaaccat atccataatt ccagcactgc       360
     aggttggaag tgaagatgag ggagatgtct acaaaatgta gtgtctatgc aattttcttg       420
     tctacgcaca ggagtgcaca gggtgggatt tgaagttcaa gttgccgaga ggcatggatc       480
     tcagtgcatg ctacttgggg tacagctggc atgtcaccca ggaaacatca acaatggtgg       540
     gaattaaata tcatggcact tatatttttt t                                      571
//