ID   CV303005   standard; mRNA; EST; 746 BP.
XX
AC   CV303005;
XX
SV   CV303005.1
XX
DT   24-SEP-2004 (Rel. 81, Created)
DT   24-SEP-2004 (Rel. 81, Last updated, Version 1)
XX
DE   CS_hyp_27h02_M13Reverse Blue crab hypodermis, normalized Callinectes
DE   sapidus cDNA clone CS_hyp_27h02 5' similar to ref|ZP_00089843.1| COG0009:
DE   Putative translation factor (SUA5) [Azotobacter. Score = 34.3 bits (77),
DE   Expect = 2.8, mRNA sequence.
XX
KW   EST.
XX
OS   Callinectes sapidus (blue crab)
OC   Eukaryota; Metazoa; Arthropoda; Crustacea; Malacostraca; Eumalacostraca;
OC   Eucarida; Decapoda; Pleocyemata; Brachyura; Eubrachyura; Portunoidea;
OC   Portunidae; Callinectes.
XX
RN   [1]
RP   1-746
RA   Shafer T.H., Coblentz F.E., Towle D.W.;
RT   "Expressed sequence tags from normalized cDNA libraries prepared from gill
RT   and hypodermis tissues of the blue crab, Callinectes sapidus";
RL   Unpublished.
XX
DR   UNILIB; 38039; 16351.
XX
CC   Contact: Thomas H. Shafer
CC   Department of Biological Sciences
CC   University of North Carolina Wilmington
CC   601 S. College Rd, Wilmington, NC 28403, USA
CC   Tel: 910-962-7275
CC   Fax: 910-962-4066
CC   Email: shafert@uncw.edu
CC   Plate: 27  row: h  column: 02
CC   Seq primer: M13 Reverse
CC   High quality sequence stop: 491.
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..746
FT                   /db_xref="taxon:6763"
FT                   /db_xref="UNILIB:38039"
FT                   /mol_type="mRNA"
FT                   /note="Vector: pCMV Sport 6.1; Total RNA samples were
FT                   prepared individually from each tissue, checked for
FT                   quality, and then pooled for construction and normalization
FT                   of a cDNA library by Invitrogen. Plasmids were isolated and
FT                   inserts sequenced from their 5'-ends by the Blue Crab
FT                   Molecular Genetics Laboratory at the University of North
FT                   Carolina Wilmington. Traces were trimmed, compared (BLASTx)
FT                   to NCBI non-redundant protein database as of 19 July 2004,
FT                   and processed for submission to dbEST by trace2dbEST
FT                   software (Parkinson, Anthony and Blaxter, unpublished
FT                   software)."
FT                   /organism="Callinectes sapidus"
FT                   /clone="CS_hyp_27h02"
FT                   /tissue_type="Pooled hypodermal epithelium from the
FT                   mid-dorsal region and arthrodial membrane of premolt (stage
FT                   D2) and 3-hour postmolt crabs"
FT                   /dev_stage="Adult"
FT                   /clone_lib="Blue crab hypodermis, normalized"
XX
SQ   Sequence 746 BP; 229 A; 155 C; 160 G; 202 T; 0 other;
     atcggaatta tcttgtatag tttgatgaga aaatgtcaca taacagaccc tcgagtaaaa        60
     tacaaaaaaa aaattgaagt tcaataataa agtaaagtcg aagggatcag tggtgttgga       120
     aacggtacat tcagaaccag ttcttgttgc cacacggtta aattatttga ctttcgattt       180
     gtttccttca tctctgaatc acagtcatga attaagaacc attattactg actgtgtata       240
     aaaagaagaa tctttggaaa agctgttgga aattaacagt aaaatgaaat cgacgaataa       300
     atcaactgat ctcttataaa aacaaatcaa agaacgttgt tacaaaaaga caagtatgcg       360
     tgctataaaa actagttcag tattacaaaa agcacaaata cttaataaaa tgttacaaac       420
     aaaaacgcac cgcttcggtg tgctgtgtgt atgtgtgtgt gtgctctgtg ccggagtggc       480
     gagtgtgcag tagttagcga gacacactcg tccccgtgcc cgcgtgtgcc gcgtgccgag       540
     tgccgcgtgc cacgtgcctc tttctctacc caggtgctcg cagtcacctg gtcacgtaac       600
     cggacaatgt atcgctccag cacgctctgt tacgccccgt cacgccacac tgcgtcacat       660
     tgccgtcagt gaggagactc ttggttgatt ttctgttgta ccacgacagt agttcaacaa       720
     cggaccgata ggtctggtgt atattt                                            746
//