ID   CV434139   standard; mRNA; EST; 728 BP.
XX
AC   CV434139;
XX
SV   CV434139.1
XX
DT   30-SEP-2004 (Rel. 81, Created)
DT   30-SEP-2004 (Rel. 81, Last updated, Version 1)
XX
DE   CS_hyp_33g01_M13Reverse Blue crab hypodermis, normalized Callinectes
DE   sapidus cDNA clone CS_hyp_33g01 5' similar to ref|ZP_00090884.1| COG0500:
DE   SAM-dependent methyltransferases - Azotobacter vinelandii. Score = 35.0
DE   bits (79), Expect = 1.6, mRNA sequence.
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KW   EST.
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OS   Callinectes sapidus (blue crab)
OC   Eukaryota; Metazoa; Arthropoda; Crustacea; Malacostraca; Eumalacostraca;
OC   Eucarida; Decapoda; Pleocyemata; Brachyura; Eubrachyura; Portunoidea;
OC   Portunidae; Callinectes.
XX
RN   [1]
RP   1-728
RA   Shafer T.H., Coblentz F.E., Towle D.W.;
RT   "Expressed sequence tags from normalized cDNA libraries prepared from gill
RT   and hypodermis tissues of the blue crab, Callinectes sapidus";
RL   Unpublished.
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DR   UNILIB; 38039; 16351.
XX
CC   Contact: Thomas H. Shafer
CC   Department of Biological Sciences
CC   University of North Carolina Wilmington
CC   601 S. College Rd, Wilmington, NC 28403, USA
CC   Tel: 910-962-7275
CC   Fax: 910-962-4066
CC   Email: shafert@uncw.edu
CC   Plate: 33  row: g  column: 01
CC   Seq primer: M13 Reverse
CC   High quality sequence stop: 464.
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..728
FT                   /db_xref="taxon:6763"
FT                   /db_xref="UNILIB:38039"
FT                   /mol_type="mRNA"
FT                   /note="Vector: pCMV Sport 6.1; Total RNA samples were
FT                   prepared individually from each tissue, checked for
FT                   quality, and then pooled for construction and normalization
FT                   of a cDNA library by Invitrogen. Plasmids were isolated and
FT                   inserts sequenced from their 5'-ends by the Blue Crab
FT                   Molecular Genetics Laboratory at the University of North
FT                   Carolina Wilmington. Traces were trimmed, compared (BLASTx)
FT                   to NCBI non-redundant protein database as of 19 July 2004,
FT                   and processed for submission to dbEST by trace2dbEST
FT                   software (Parkinson, Anthony and Blaxter, unpublished
FT                   software)."
FT                   /organism="Callinectes sapidus"
FT                   /clone="CS_hyp_33g01"
FT                   /tissue_type="Pooled hypodermal epithelium from the
FT                   mid-dorsal region and arthrodial membrane of premolt (stage
FT                   D2) and 3-hour postmolt crabs"
FT                   /dev_stage="Adult"
FT                   /clone_lib="Blue crab hypodermis, normalized"
XX
SQ   Sequence 728 BP; 187 A; 232 C; 182 G; 127 T; 0 other;
     tgacggagga acacacgagc agaggaacga acacccgctg tgagatgatg cgctacgtgt        60
     ttctgctggc ggcgctggtg gtgggcgatg cgtggggccg cccttcagag actctgcaac       120
     tctccaccac gcctcccacg gctacgatgc caacggtgca gctcatcaag aacctgggaa       180
     acctcaagat ccacgtgaca cagcagcctg aaggggacta cgggctggaa gtgtacgaca       240
     acaaactgga gaagctggtg ccctccacgg tcttctcact gcctggagac tggaaacccc       300
     ccggcctgga gaagtcccgc attgtgcacg ctgagttcct ggacagcggg gatgtggtgg       360
     tggccttctt cagcctcgag aacagcctgg acctcatcct gctgcccaag gaactattcg       420
     tctacaagaa aacaaactca agcgtgattg tcttcccgaa gcttgatgtc ccttggcacc       480
     ccactgtgtc cacgccgacc gagtactgga aatggcctga caccaccaca ccctacacca       540
     acaccaccac caccacagcg gcctacaaca ctacacccta cactaccaca ccctacacca       600
     ccaccacagc ggcctacacc actacaagtt atcgcacgcc ggtagttgag tagaggtagc       660
     gggtgacagc aactcaacac aacacaacac agcaactcag tattgtgata tatagtggaa       720
     ggcttatg                                                                728
//