ID   CV434425   standard; mRNA; EST; 726 BP.
XX
AC   CV434425;
XX
SV   CV434425.1
XX
DT   30-SEP-2004 (Rel. 81, Created)
DT   30-SEP-2004 (Rel. 81, Last updated, Version 1)
XX
DE   CS_hyp_37b07_M13Reverse Blue crab hypodermis, normalized Callinectes
DE   sapidus cDNA clone CS_hyp_37b07 5' similar to ref|ZP_00089843.1| COG0009:
DE   Putative translation factor (SUA5) [Azotobacter. Score = 35.0 bits (79),
DE   Expect = 1.5, mRNA sequence.
XX
KW   EST.
XX
OS   Callinectes sapidus (blue crab)
OC   Eukaryota; Metazoa; Arthropoda; Crustacea; Malacostraca; Eumalacostraca;
OC   Eucarida; Decapoda; Pleocyemata; Brachyura; Eubrachyura; Portunoidea;
OC   Portunidae; Callinectes.
XX
RN   [1]
RP   1-726
RA   Shafer T.H., Coblentz F.E., Towle D.W.;
RT   "Expressed sequence tags from normalized cDNA libraries prepared from gill
RT   and hypodermis tissues of the blue crab, Callinectes sapidus";
RL   Unpublished.
XX
DR   UNILIB; 38039; 16351.
XX
CC   Contact: Thomas H. Shafer
CC   Department of Biological Sciences
CC   University of North Carolina Wilmington
CC   601 S. College Rd, Wilmington, NC 28403, USA
CC   Tel: 910-962-7275
CC   Fax: 910-962-4066
CC   Email: shafert@uncw.edu
CC   Plate: 37  row: b  column: 07
CC   Seq primer: M13 Reverse
CC   High quality sequence stop: 495.
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..726
FT                   /db_xref="taxon:6763"
FT                   /db_xref="UNILIB:38039"
FT                   /mol_type="mRNA"
FT                   /note="Vector: pCMV Sport 6.1; Total RNA samples were
FT                   prepared individually from each tissue, checked for
FT                   quality, and then pooled for construction and normalization
FT                   of a cDNA library by Invitrogen. Plasmids were isolated and
FT                   inserts sequenced from their 5'-ends by the Blue Crab
FT                   Molecular Genetics Laboratory at the University of North
FT                   Carolina Wilmington. Traces were trimmed, compared (BLASTx)
FT                   to NCBI non-redundant protein database as of 19 July 2004,
FT                   and processed for submission to dbEST by trace2dbEST
FT                   software (Parkinson, Anthony and Blaxter, unpublished
FT                   software)."
FT                   /organism="Callinectes sapidus"
FT                   /clone="CS_hyp_37b07"
FT                   /tissue_type="Pooled hypodermal epithelium from the
FT                   mid-dorsal region and arthrodial membrane of premolt (stage
FT                   D2) and 3-hour postmolt crabs"
FT                   /dev_stage="Adult"
FT                   /clone_lib="Blue crab hypodermis, normalized"
XX
SQ   Sequence 726 BP; 224 A; 147 C; 156 G; 199 T; 0 other;
     gtaaaataaa aaaaaaaaat tgaagttcaa taataaagta aagtcgaagg gatcagtggt        60
     gttggaaacg gtacattcag aaccagttct tgttgccaca cggttaaatt atttgacttt       120
     cgatttgttt ccttcatctc tgaatcacag tcttgaatta agaaccatta ttactgactg       180
     tgtataaaaa gaagaatctt tggaaaagct gttggaaatt aacagtaaaa tgaaatcgac       240
     gaataaatca actgatctct tataaaaaca aatcaaagaa cgttgttaca aaaagacaag       300
     tatgcgtgct ataaaaacta gttcagtatt acaaaaagca caaatactta ataaaatgtt       360
     acaaacaaaa acgcaccgct tcggtgtgct gtgtgtatgt gtgtgtgtgc tctgtgccgg       420
     agtggcgagt gtgcagtagt tagcgagaca cactcgaccc cgtgcccgcg tgtgccgcgt       480
     gccgagtgcc gcgtgccacg tgcctctttc tctacccagg tgctcgcagt cacctggtca       540
     cgtaacctga cgatgtatcg ctccagcacg ctctgttacg ccccgtcacg ccacactgcg       600
     tcacattgcc gtcagtgagg agactcttgg ttgattttct gttgcaccac gacagtagtt       660
     caacaacgga ccgataggtc tggtgtatat ttttgtatag ataaaagttt tctgtaaatg       720
     atgaaa                                                                  726
//