ID   CV479286   standard; mRNA; EST; 646 BP.
XX
AC   CV479286;
XX
SV   CV479286.1
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DT   02-OCT-2004 (Rel. 81, Created)
DT   02-OCT-2004 (Rel. 81, Last updated, Version 1)
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DE   CS_hyp_42b07_M13Reverse Blue crab hypodermis, normalized Callinectes
DE   sapidus cDNA clone CS_hyp_42b07 5' similar to ref|ZP_00091652.1|
DE   hypothetical protein - Azotobacter vinelandii. Score = 36.2 bits (82),
DE   Expect = 0.55, mRNA sequence.
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KW   EST.
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OS   Callinectes sapidus (blue crab)
OC   Eukaryota; Metazoa; Arthropoda; Crustacea; Malacostraca; Eumalacostraca;
OC   Eucarida; Decapoda; Pleocyemata; Brachyura; Eubrachyura; Portunoidea;
OC   Portunidae; Callinectes.
XX
RN   [1]
RP   1-646
RA   Shafer T.H., Coblentz F.E., Towle D.W.;
RT   "Expressed sequence tags from normalized cDNA libraries prepared from gill
RT   and hypodermis tissues of the blue crab, Callinectes sapidus";
RL   Unpublished.
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DR   UNILIB; 38039; 16351.
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CC   Contact: Thomas H. Shafer
CC   Department of Biological Sciences
CC   University of North Carolina Wilmington
CC   601 S. College Rd, Wilmington, NC 28403, USA
CC   Tel: 910-962-7275
CC   Fax: 910-962-4066
CC   Email: shafert@uncw.edu
CC   Plate: 42  row: b  column: 07
CC   Seq primer: M13 Reverse
CC   High quality sequence stop: 495.
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FH   Key             Location/Qualifiers
FH
FT   source          1..646
FT                   /db_xref="taxon:6763"
FT                   /db_xref="UNILIB:38039"
FT                   /mol_type="mRNA"
FT                   /note="Vector: pCMV Sport 6.1; Total RNA samples were
FT                   prepared individually from each tissue, checked for
FT                   quality, and then pooled for construction and normalization
FT                   of a cDNA library by Invitrogen. Plasmids were isolated and
FT                   inserts sequenced from their 5'-ends by the Blue Crab
FT                   Molecular Genetics Laboratory at the University of North
FT                   Carolina Wilmington. Traces were trimmed, compared (BLASTx)
FT                   to NCBI non-redundant protein database as of 19 July 2004,
FT                   and processed for submission to dbEST by trace2dbEST
FT                   software (Parkinson, Anthony and Blaxter, unpublished
FT                   software)."
FT                   /organism="Callinectes sapidus"
FT                   /clone="CS_hyp_42b07"
FT                   /tissue_type="Pooled hypodermal epithelium from the
FT                   mid-dorsal region and arthrodial membrane of premolt (stage
FT                   D2) and 3-hour postmolt crabs"
FT                   /dev_stage="Adult"
FT                   /clone_lib="Blue crab hypodermis, normalized"
XX
SQ   Sequence 646 BP; 183 A; 216 C; 178 G; 69 T; 0 other;
     cgaaaatgag gactctacta ccaccaccac cacaacggca aaaccacgcc gtcgcggtaa        60
     caggccgagg ggcaacaaca ggcagagaca aggcggccca gggggacgac aagatgacgc       120
     agaggagcgc gcaggaaccg gaggagccag caggcgagga ggtggaggag gacgaggtgg       180
     tggcggagcg acgggaggaa gaaatcgaca gcgccaaagg gaaagtccat ccaataccgc       240
     gggagagccc tccgactccc gcttctcccg cctacaagca ggaggttcac gagaccagga       300
     aggggcggac ggcggcaacc aggatcccca gcccgatgcg tccacaggat ctcgcagacg       360
     gcggccaaac cgtccccgtc ccaagataaa cttcggcagc atttttttta ccaccaccac       420
     caccactaca accactaccg aggccacaga ggactattat tatgatgaat attatgacta       480
     ttaccaggat aatcaagaag aaaccaccac caccaccacc accaccacaa cagaggcttc       540
     ctcccgccgc tcccgcccct cccgcacacg agctcgcggc ggcagtgatg ccaccgccac       600
     cgagcaagac gcacccgcag aagaatcccc tgccactgga gcacgg                      646
//