ID   CV480086   standard; mRNA; EST; 759 BP.
XX
AC   CV480086;
XX
SV   CV480086.1
XX
DT   02-OCT-2004 (Rel. 81, Created)
DT   02-OCT-2004 (Rel. 81, Last updated, Version 1)
XX
DE   CS_hyp_65d05_M13Reverse Blue crab hypodermis, normalized Callinectes
DE   sapidus cDNA clone CS_hyp_65d05 5' similar to ref|ZP_00092405.1| COG1538:
DE   Outer membrane protein - Azotobacter vinelandii. Score = 40.8 bits (94),
DE   Expect = 0.031, mRNA sequence.
XX
KW   EST.
XX
OS   Callinectes sapidus (blue crab)
OC   Eukaryota; Metazoa; Arthropoda; Crustacea; Malacostraca; Eumalacostraca;
OC   Eucarida; Decapoda; Pleocyemata; Brachyura; Eubrachyura; Portunoidea;
OC   Portunidae; Callinectes.
XX
RN   [1]
RP   1-759
RA   Shafer T.H., Coblentz F.E., Towle D.W.;
RT   "Expressed sequence tags from normalized cDNA libraries prepared from gill
RT   and hypodermis tissues of the blue crab, Callinectes sapidus";
RL   Unpublished.
XX
DR   UNILIB; 38039; 16351.
XX
CC   Contact: Thomas H. Shafer
CC   Department of Biological Sciences
CC   University of North Carolina Wilmington
CC   601 S. College Rd, Wilmington, NC 28403, USA
CC   Tel: 910-962-7275
CC   Fax: 910-962-4066
CC   Email: shafert@uncw.edu
CC   Plate: 65  row: d  column: 05
CC   Seq primer: M13 Reverse
CC   High quality sequence stop: 492.
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..759
FT                   /db_xref="taxon:6763"
FT                   /db_xref="UNILIB:38039"
FT                   /mol_type="mRNA"
FT                   /note="Vector: pCMV Sport 6.1; Total RNA samples were
FT                   prepared individually from each tissue, checked for
FT                   quality, and then pooled for construction and normalization
FT                   of a cDNA library by Invitrogen. Plasmids were isolated and
FT                   inserts sequenced from their 5'-ends by the Blue Crab
FT                   Molecular Genetics Laboratory at the University of North
FT                   Carolina Wilmington. Traces were trimmed, compared (BLASTx)
FT                   to NCBI non-redundant protein database as of 19 July 2004,
FT                   and processed for submission to dbEST by trace2dbEST
FT                   software (Parkinson, Anthony and Blaxter, unpublished
FT                   software)."
FT                   /organism="Callinectes sapidus"
FT                   /clone="CS_hyp_65d05"
FT                   /tissue_type="Pooled hypodermal epithelium from the
FT                   mid-dorsal region and arthrodial membrane of premolt (stage
FT                   D2) and 3-hour postmolt crabs"
FT                   /dev_stage="Adult"
FT                   /clone_lib="Blue crab hypodermis, normalized"
XX
SQ   Sequence 759 BP; 141 A; 282 C; 194 G; 142 T; 0 other;
     cgatacgctc ccttgtgatt gtgagtgtag tacttggcgt ggccctcggc caagttttcc        60
     ctcgcgacac cccggaggtg gaggcagccc gcaatgcctt catcgctgag tacaaccgcc       120
     tggccaggct ggcagcgctg gcacctgaca tccacatcta ccaccgcgac cgcgtgatgg       180
     accctgtcac caatgttcct aggcccatca accccccggc cttcaacgcc ttctccttct       240
     ccgccaatct gggcaccaag gagcatttcg tgcccggccc caacatcttc cctggtgcca       300
     cacacatggc aggacacctg gccggacatc atttcgcgcc cacccataag cccgcggctc       360
     gtcacccagt tgtcaccgct gccccagccc cgcacttcgc tcccactccc gtcagtcaga       420
     cgcccctgtt cgatctcgcc gccgaggagc caatgatgcg ctggacgggg cctttttctg       480
     acgaggtgcc cgccggcctg agcggacacg tgagtgagac acccagtgtt gtcgccgcca       540
     aggcagctca cttcagggcc cacgccgatt actggaacca ggcgaatagg aacaccgtca       600
     ccaagcgatg aggaggccag cagtagcagc cccagccgaa cattcaggag ccccggggag       660
     gcctacgcag tccccggccc gtgccaaaga tgacccttgt agccatgtcg catcgcattc       720
     ttatatatct tcgtggcccc gcgtcacatc tttctctcc                              759
//