ID   CV527622   standard; mRNA; EST; 623 BP.
XX
AC   CV527622;
XX
SV   CV527622.1
XX
DT   08-OCT-2004 (Rel. 81, Created)
DT   08-OCT-2004 (Rel. 81, Last updated, Version 1)
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DE   CS_GIL_06A06_SP6 Blue crab gill, normalized Callinectes sapidus cDNA clone
DE   CS_GIL_06A06 5' similar to ref|ZP_00091118.1| COG0210: Superfamily I DNA
DE   and RNA helicases [Azotobacter. Score = 42.0 bits (97), Expect = 0.009,
DE   mRNA sequence.
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KW   EST.
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OS   Callinectes sapidus (blue crab)
OC   Eukaryota; Metazoa; Arthropoda; Crustacea; Malacostraca; Eumalacostraca;
OC   Eucarida; Decapoda; Pleocyemata; Brachyura; Eubrachyura; Portunoidea;
OC   Portunidae; Callinectes.
XX
RN   [1]
RP   1-623
RA   Shafer T.H., Coblentz F.E., Towle D.W.;
RT   "Expressed sequence tags from normalized cDNA libraries prepared from gill
RT   and hypodermis tissues of the blue crab, Callinectes sapidus";
RL   Unpublished.
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DR   UNILIB; 37905; 16215.
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CC   Contact: Thomas H. Shafer
CC   Department of Biological Sciences
CC   University of North Carolina Wilmington
CC   601 S. College Rd, Wilmington, NC 28403, USA
CC   Tel: 910-962-7275
CC   Fax: 910-962-4066
CC   Email: shafert@uncw.edu
CC   Plate: 06  row: A  column: 06
CC   Seq primer: SP6
CC   High quality sequence start: 29
CC   High quality sequence stop: 537.
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FH   Key             Location/Qualifiers
FH
FT   source          1..623
FT                   /db_xref="taxon:6763"
FT                   /db_xref="UNILIB:37905"
FT                   /mol_type="mRNA"
FT                   /note="Vector: pCMV Sport 6.1; Total RNA samples were
FT                   prepared individually from each tissue, checked for
FT                   quality, and then pooled for construction and normalization
FT                   of a cDNA library by Invitrogen. Plasmids were isolated and
FT                   inserts sequenced from their 5'-ends by the Blue Crab
FT                   Molecular Genetics Laboratory at the University of North
FT                   Carolina Wilmington. Traces were trimmed, compared (BLASTx)
FT                   to NCBI non-redundant protein database as of 19 July 2004,
FT                   and processed for submission to dbEST by trace2dbEST
FT                   software (Parkinson, Anthony and Blaxter, unpublished
FT                   software)."
FT                   /organism="Callinectes sapidus"
FT                   /clone="CS_GIL_06A06"
FT                   /tissue_type="Pooled anterior and posterior gills from
FT                   crabs acclimated to salinities of 35 and 5 parts per
FT                   thousand"
FT                   /dev_stage="Adult intermolt"
FT                   /clone_lib="Blue crab gill, normalized"
XX
SQ   Sequence 623 BP; 95 A; 222 C; 217 G; 89 T; 0 other;
     caaaaagcag ctggtccggt ccgttatccc gggattggag cagtggcggt ggtgcggcgc        60
     ggggggcaga gggcaggggg gcggcgctca ctgctccatc acatgtggcc gtgagggact       120
     cgtgctgact tacaacactc atcccggctc cacctgtgcc accctttgtc cctgctgcac       180
     ccccgcccct ctctgccccc tggccgccgc ctctcccact gtgccaccct cgcccgctgc       240
     ccagcccgtc cctgtcaccg ccctgggtct tggcgagggg gcggatgttg ccaccctgag       300
     ccgcagcctg ggtgacctga gtcggccaca ggagagcagt gcggcagggc gcagcatgac       360
     cttcagcggc atcttggacc gtcacacgcc acgctcaggc ccgcagcgtc cctacggccg       420
     ccgctgcaag agcacggcgc acattgtgct gcagaacaac gacacggtga aggagccgcg       480
     cccccacgag gtgcacgccg cctcgggtac acacctgcac gtgccctcgg gtgaggcacg       540
     agggcgtgcc aggcaccgga ccgcccctgc ggcagggagg ggagagaggg gcggctggca       600
     ccgggtggag ggtgtagggc gag                                               623
//