ID   CV527776   standard; mRNA; EST; 660 BP.
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AC   CV527776;
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SV   CV527776.1
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DT   08-OCT-2004 (Rel. 81, Created)
DT   08-OCT-2004 (Rel. 81, Last updated, Version 1)
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DE   CS_GIL_19F04_SP6 Blue crab gill, normalized Callinectes sapidus cDNA clone
DE   CS_GIL_19F04 5' similar to ref|ZP_00092479.1| COG1960: Acyl-CoA
DE   dehydrogenases - Azotobacter vinelandii. Score = 32.7 bits (73), Expect =
DE   6.3, mRNA sequence.
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KW   EST.
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OS   Callinectes sapidus (blue crab)
OC   Eukaryota; Metazoa; Arthropoda; Crustacea; Malacostraca; Eumalacostraca;
OC   Eucarida; Decapoda; Pleocyemata; Brachyura; Eubrachyura; Portunoidea;
OC   Portunidae; Callinectes.
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RN   [1]
RP   1-660
RA   Shafer T.H., Coblentz F.E., Towle D.W.;
RT   "Expressed sequence tags from normalized cDNA libraries prepared from gill
RT   and hypodermis tissues of the blue crab, Callinectes sapidus";
RL   Unpublished.
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DR   UNILIB; 37905; 16215.
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CC   Contact: Thomas H. Shafer
CC   Department of Biological Sciences
CC   University of North Carolina Wilmington
CC   601 S. College Rd, Wilmington, NC 28403, USA
CC   Tel: 910-962-7275
CC   Fax: 910-962-4066
CC   Email: shafert@uncw.edu
CC   Plate: 19  row: F  column: 04
CC   Seq primer: SP6
CC   High quality sequence start: 4
CC   High quality sequence stop: 513.
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FH   Key             Location/Qualifiers
FH
FT   source          1..660
FT                   /db_xref="taxon:6763"
FT                   /db_xref="UNILIB:37905"
FT                   /mol_type="mRNA"
FT                   /note="Vector: pCMV Sport 6.1; Total RNA samples were
FT                   prepared individually from each tissue, checked for
FT                   quality, and then pooled for construction and normalization
FT                   of a cDNA library by Invitrogen. Plasmids were isolated and
FT                   inserts sequenced from their 5'-ends by the Blue Crab
FT                   Molecular Genetics Laboratory at the University of North
FT                   Carolina Wilmington. Traces were trimmed, compared (BLASTx)
FT                   to NCBI non-redundant protein database as of 19 July 2004,
FT                   and processed for submission to dbEST by trace2dbEST
FT                   software (Parkinson, Anthony and Blaxter, unpublished
FT                   software)."
FT                   /organism="Callinectes sapidus"
FT                   /clone="CS_GIL_19F04"
FT                   /tissue_type="Pooled anterior and posterior gills from
FT                   crabs acclimated to salinities of 35 and 5 parts per
FT                   thousand"
FT                   /dev_stage="Adult intermolt"
FT                   /clone_lib="Blue crab gill, normalized"
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SQ   Sequence 660 BP; 167 A; 144 C; 193 G; 156 T; 0 other;
     atgtgcggcg tgtgacggac tgacggtgcg accattccgc ggcgggactg aggcgcgagg        60
     gggtcaggca ctctcactct gctctctgct ctacacggac cgcgctatcg gattgcttta       120
     tttaatgtac gagagttgtg caaaggaagc gaaaactgtc gtgtgaatgt gtctgcgtgt       180
     gtgtttcagt atagatactg tgctgctgcc aggttttggt aaccagcaaa gagttgcctt       240
     aaggtcctca atttgcagat tgaagagatt ctgcgttatt ctttaaacgt gagacgaaac       300
     cacatggaaa aacttttatc atcacgactg cagcttgact tgtgtttggg ggagtaaaag       360
     gagaaataaa gactacagaa tagagaagac gaggaagaaa cgcaacaaca ccattatcaa       420
     caccatcttc accaccacca ccaccgaaaa ccgcacccac aagacccatc tcagcgcggc       480
     aggagcgttg aagtgagtgt tgaatagagg cgggcgaact tttcatgcac gggcgggact       540
     cgctagcatg acgtggacag actttgtgga atggctggat caaaacaagg ttgtgcttat       600
     cgccgtgacc tgttcagtgg tgggtgccgg tgggtgtctg gtgggtttgc gggcatggct       660
//