ID   ALGI_AZOVI     STANDARD;      PRT;   499 AA.
AC   O52196;
DT   10-OCT-2003 (Rel. 42, Created)
DT   10-OCT-2003 (Rel. 42, Last sequence update)
DT   05-JUL-2004 (Rel. 44, Last annotation update)
DE   Probable poly(beta-D-mannuronate) O-acetylase (EC 2.3.1.-) (Alginate
DE   biosynthesis protein algI).
GN   Name=algI;
OS   Azotobacter vinelandii.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Azotobacter.
OX   NCBI_TaxID=354;
RN   [1]
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=ATCC 9046;
RX   MEDLINE=99282527; PubMed=10352233; DOI=10.1016/S0378-1119(99)00119-5;
RA   Vazquez A., Moreno S., Guzman J., Alvarado A., Espin G.;
RT   "Transcriptional organization of the Azotobacter vinelandii algGXLVIFA
RT   genes: characterization of algF mutants.";
RL   Gene 232:217-222(1999).
CC   -!- FUNCTION: Together with algV and algF, forms an inner membrane
CC       complex which probably interacts with the alginate polymerization-
CC       transport complex and adds acetyl groups at the O-2 and O-3
CC       positions of polymannuronic acid. Acetylation of alginate
CC       increases cyst resistance to desiccation.
CC   -!- PATHWAY: Alginate biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Integral membrane protein. Inner membrane
CC       (By similarity).
CC   -!- SIMILARITY: Belongs to the membrane-bound acyltransferase family.
CC   --------------------------------------------------------------------------
CC   This Swiss-Prot entry is copyright. It is produced through a collaboration
CC   between  the Swiss Institute of Bioinformatics  and the  EMBL outstation -
CC   the European Bioinformatics Institute.  There are no  restrictions on  its
CC   use as long as its content is in no way modified and this statement is not
CC   removed.
CC   --------------------------------------------------------------------------
DR   EMBL; AF027499; AAC04568.1; -.
DR   InterPro; IPR004299; MBOAT_fam.
DR   Pfam; PF03062; MBOAT; 1.
KW   Acyltransferase; Alginate biosynthesis; Inner membrane; Transferase;
KW   Transmembrane.
FT   TRANSMEM      7     25       Potential.
FT   TRANSMEM     40     62       Potential.
FT   TRANSMEM     78    100       Potential.
FT   TRANSMEM    115    137       Potential.
FT   TRANSMEM    150    172       Potential.
FT   TRANSMEM    239    261       Potential.
FT   TRANSMEM    312    334       Potential.
FT   TRANSMEM    354    373       Potential.
FT   TRANSMEM    380    397       Potential.
FT   TRANSMEM    407    429       Potential.
FT   TRANSMEM    475    497       Potential.
FT   ACT_SITE    322    322       Potential.
SQ   SEQUENCE   499 AA;  56387 MW;  CBB74AE4DF08F63C CRC64;
     MVFSSNVFLF LFLPLFLGLY YLSPARYRNL LLLTASYVFY AWWRIDFLGL FAAVTVFNYW
     IGLRIGAAGV RTQSAQRWLT LGVVVDLCVL GYFKYANFGV DSLNAIITSF GVEPFVVTHI
     LLPIGISFYV FESISYIIDV YRGDTPATRN LVDFAAFVAI FPHLIAGPVL RFRDLVDQFN
     HRTHTLDKFA EGCTRFMQGF IKKVFIADSL APIADHCFAL SDPTTGDAWL GALAYTAQLY
     FDFSGYSDMA IGLGLMIGFR FMENFNQPYI SQSITEFWRR WHISLSTWLR DYLYISLGGN
     RGTTFQTYRN LILTMLLGGL WHGANWTFII WGAWHGTWLA IERALRIDAA PKTIRPLRWV
     FAFLLVMVGW VIFRAENLDV AWRMYAAMFS FGDWTLSDLN KAQLTSLQIA TLLLAYVVIA
     VYGIRQFYAQ PLTGAPKAKA NDQADAPQGI VHASSGTLAY SQAIDIPALA TRVAVLLLFA
     ASVLKLSAQS FSPFLYFQF
//