ID   FLAV_AZOVI     STANDARD;      PRT;   179 AA.
AC   P00324;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   21-JUL-1986, sequence version 1.
DT   16-MAY-2006, entry version 56.
DE   Flavodoxin-2.
GN   Name=nifF;
OS   Azotobacter vinelandii.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Azotobacter.
OX   NCBI_TaxID=354;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   MEDLINE=89123097; PubMed=2644218;
RA   Jacobson M.R., Brigle K.E., Bennett L.T., Setterquist R.A.,
RA   Wilson M.S., Cash V.L., Beynon J., Newton W.E., Dean D.R.;
RT   "Physical and genetic map of the major nif gene cluster from
RT   Azotobacter vinelandii.";
RL   J. Bacteriol. 171:1017-1027(1989).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   MEDLINE=88087273; PubMed=3121629;
RA   Bennett L., Jacobson M., Dean D.R.;
RT   "Isolation, sequencing, and mutagenesis of the nifF gene encoding
RT   flavodoxin from Azotobacter vinelandii.";
RL   J. Biol. Chem. 263:1364-1369(1988).
RN   [3]
RP   PROTEIN SEQUENCE.
RC   STRAIN=ATCC 13705 / OP1 / DSM 366 / NCIB 11614 / LMG 3878 / UW;
RX   MEDLINE=77242321; PubMed=889809;
RA   Tanaka M., Haniu M., Yasunobu K.T., Yoch D.C.;
RT   "Complete amino acid sequence of azotoflavin, a flavodoxin from
RT   Azotobacter vinelandii.";
RL   Biochemistry 16:3525-3537(1977).
RN   [4]
RP   PROTEIN SEQUENCE OF 1-20, AND MASS SPECTROMETRY.
RC   STRAIN=OP / UW136;
RX   MEDLINE=96276406; PubMed=8694750;
RA   Gangeswaran R., Eady R.R.;
RT   "Flavodoxin 1 of Azotobacter vinelandii: characterization and role in
RT   electron donation to purified assimilatory nitrate reductase.";
RL   Biochem. J. 317:103-108(1996).
RN   [5]
RP   STRUCTURE BY NMR.
RC   STRAIN=ATCC 478 / NRS 16 / DSM 2289 / VKM B-1617;
RX   MEDLINE=98180401; PubMed=9521106;
RA   Steensma E., Nijman M.J.M., Bollen Y.J.M., de Jager P.A.,
RA   van den Berg W.A.M., van Dongen W.M.A.M., van Mierlo C.P.M.;
RT   "Apparent local stability of the secondary structure of Azotobacter
RT   vinelandii holoflavodoxin II as probed by hydrogen exchange:
RT   implications for redox potential regulation and flavodoxin folding.";
RL   Protein Sci. 7:306-317(1998).
CC   -!- FUNCTION: Low-potential electron donor to a number of redox
CC       enzymes. NifF is the electron donor to nitrogenase.
CC   -!- COFACTOR: FMN.
CC   -!- SUBUNIT: Monomer.
CC   -!- MASS SPECTROMETRY: MW=19533; MW_ERR=5; METHOD=Electrospray;
CC       RANGE=1-179; NOTE=Ref.4.
CC   -!- SIMILARITY: Belongs to the flavodoxin family.
CC   -!- SIMILARITY: Contains 1 flavodoxin-like domain.
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DR   EMBL; M20568; AAA64735.1; -; Genomic_DNA.
DR   EMBL; J03519; AAA22154.1; -; Genomic_DNA.
DR   PIR; A29935; FXAVEP.
DR   PDB; 1YOB; X-ray; A/B=1-179.
DR   InterPro; IPR010086; Flav_long.
DR   InterPro; IPR001094; Flavdoxin_like.
DR   InterPro; IPR001226; Flavodoxin.
DR   InterPro; IPR008254; Flavodoxin_1.
DR   Pfam; PF00258; Flavodoxin_1; 1.
DR   PRINTS; PR00369; FLAVODOXIN.
DR   TIGRFAMs; TIGR01752; flav_long; 1.
DR   PROSITE; PS00201; FLAVODOXIN; 1.
DR   PROSITE; PS50902; FLAVODOXIN_LIKE; 1.
KW   3D-structure; Direct protein sequencing; Electron transport;
KW   Flavoprotein; FMN; Nitrogen fixation; Transport.
FT   INIT_MET      0      0
FT   CHAIN         1    179       Flavodoxin-2.
FT                                /FTId=PRO_0000171605.
FT   DOMAIN        3    172       Flavodoxin-like.
SQ   SEQUENCE   179 AA;  19532 MW;  661B0EC667375A7F CRC64;
     AKIGLFFGSN TGKTRKVAKS IKKRFDDETM SDALNVNRVS AEDFAQYQFL ILGTPTLGEG
     ELPGLSSDCE NESWEEFLPK IEGLDFSGKT VALFGLGDQV GYPENYLDAL GELYSFFKDR
     GAKIVGSWST DGYEFESSEA VVDGKFVGLA LDLDNQSGKT DERVAAWLAQ IAPEFGLSL
//