ID   ANFA_AZOVI     STANDARD;      PRT;   533 AA.
AC   P12626;
DT   01-OCT-1989 (Rel. 12, Created)
DT   01-OCT-1989 (Rel. 12, Last sequence update)
DT   05-JUL-2004 (Rel. 44, Last annotation update)
DE   Nitrogen fixation protein anfA.
GN   Name=anfA;
OS   Azotobacter vinelandii.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Azotobacter.
OX   NCBI_TaxID=354;
RN   [1]
RP   NUCLEOTIDE SEQUENCE.
RX   MEDLINE=89255091; PubMed=2722750;
RA   Joerger R.D., Jacobson M.R., Bishop P.E.;
RT   "Two nifA-like genes required for expression of alternative
RT   nitrogenases by Azotobacter vinelandii.";
RL   J. Bacteriol. 171:3258-3267(1989).
CC   -!- FUNCTION: AnfA is essential for nitrogen fixation under Mo- and V-
CC       deficient conditions. It is required for the regulation of
CC       nitrogenase 3 transcription. Interacts with sigma-54.
CC   -!- SIMILARITY: Contains 1 sigma-54 factor interaction ATP-binding
CC       domain.
CC   --------------------------------------------------------------------------
CC   This Swiss-Prot entry is copyright. It is produced through a collaboration
CC   between  the Swiss Institute of Bioinformatics  and the  EMBL outstation -
CC   the European Bioinformatics Institute.  There are no  restrictions on  its
CC   use as long as its content is in no way modified and this statement is not
CC   removed.
CC   --------------------------------------------------------------------------
DR   EMBL; M26751; AAA22146.1; -.
DR   PIR; A44514; A44514.
DR   InterPro; IPR003593; AAA_ATPase.
DR   InterPro; IPR003018; GAF.
DR   InterPro; IPR002197; HTH_Fis.
DR   InterPro; IPR002078; Sig54_interact.
DR   Pfam; PF01590; GAF; 1.
DR   Pfam; PF02954; HTH_8; 1.
DR   Pfam; PF00158; Sigma54_activat; 1.
DR   PRINTS; PR01590; HTHFIS.
DR   SMART; SM00382; AAA; 1.
DR   SMART; SM00065; GAF; 1.
DR   TIGRFAMs; TIGR01199; HTH_fis; 1.
DR   PROSITE; PS00675; SIGMA54_INTERACT_1; 1.
DR   PROSITE; PS00676; SIGMA54_INTERACT_2; 1.
DR   PROSITE; PS00688; SIGMA54_INTERACT_3; 1.
DR   PROSITE; PS50045; SIGMA54_INTERACT_4; 1.
KW   Activator; ATP-binding; DNA-binding; Nitrogen fixation;
KW   Transcription regulation; Two-component regulatory system.
FT   DOMAIN       33    193       A domain.
FT   DOMAIN      219    448       Sigma-54 factor interaction (Potential).
FT   NP_BIND     247    254       ATP (Potential).
FT   NP_BIND     310    319       ATP (Potential).
FT   DNA_BIND    501    520       H-T-H motif.
SQ   SEQUENCE   533 AA;  59547 MW;  0DEFBE02A1CF7695 CRC64;
     MSDQATFEFD TDYFVEEFSH CFTGECRVKM LPILYKISQI ITGNADLADA LSIVLGVMQQ
     HLKMQRGIVT LYDMRAETIF IHDSFGLTEE EKKRGIYAVG EGITGKVVET GKAIVARRLQ
     EHPDFLGRTR VSRNGKAKAA FFCVPIMRAQ KVLGTIAAER VYMNPRLLKQ DVELLTMIAT
     MIAPLVELYL IENIERVRLE NENRRLKHAL KERFKPSNII GNSKPMQEVY ELIHKVASTK
     ATVLILGESG VGKELVANAI HYNSPNAEAA LVTSNCAPLP ENLAESELFG HEKGSFTGAL
     TMHKGCFEQA DGGTIFLDEV GELSPTVQAK LVRVLQNRTF ERVGGSKPVK VDVRIIAATN
     RNLVEMVEQG TFREDLYYRL NVFPITVPPL RERGSDVIAL ADHFVSAFSR ENGKNVKRIS
     TPALNMLMSY HWPGNVRELE NVMERAVILS DDDVIHSYNL PPSLQTSKES GTAFGLTLEE
     KIKAVECEMI VEALKNSSGH IGEAAKELGL ARRMLGVRME RYGISYKSFS RYA
//